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ABSTRACT: On-line monitoring of mammalian cells cultures used in the production of various biologicals is important for the optimisation
and control of these processes. Besides traditional sensors such as pH, temperature and dissolved oxygen the viable biomass
in cell cultures could be monitored on-line. Dielectric spectroscopy or capacitance, offers an attractive tool for the estimation
of this parameter; it has the advantage of only measuring the viable cell density.
In this work we characterized Vero cells culture process for the production of rabies virus with dielectric spectroscopy technique
using multi-frequency scanning and raw spectral data of cultures obtained as raw signatures.
Cells were grown on Cytodex 1 microcarriers in stirred bioreactor. Cell permitivity under different growth/infection conditions
were investigated. On line permitivity data were compared with off line cell density level determined by the crystal violet
method, during the cell growth and virus replication phase.
Interpretation of raw signature obtained to track on-line cellular physiology changes and any specific information will be
demonstrated to improve process itself.
Signatures obtained will be used for better real-time control of critical aspects, detect and diagnose abnormal culture conditions
and as a tool for PAT implementation.
KeywordsBioreactor-Dielectric spectroscopy-Multifrequency scanning-Rabies virus-Vero cells
12/2009: pages 829-832;
