[show abstract][hide abstract] ABSTRACT: An efficient method for the simultaneous extraction and quantification of both jasmonic acid (JA) and methyl jasmonate (MeJA)
from a single plant sample was developed. Plant tissues were firstly extracted by using 100% cold methanol, then the Sep-pak
C18 solid-phase extraction (SPE) cartridges were adopted for the purification of sample extract, and finally JA and MeJA were
determined by the liquid chromatography tandem mass spectrometry (LC-MS-MS) system. It was found that the accuracy was improved
by using the extra standard for the estimation and correction of JA and MeJA losses in the extraction and purification process.
The detection limits for JA and MeJA were 0.03 and 0.075 ng mL−1, respectively, and the average recovery rate of JA and MeJA was 92.48% and 94.30%. The method was found to be reproducible
and selective, yielding well isolated and easily detectable peaks for both JA and MeJA and simplifying the time-consuming
extraction and purification. High-performance liquid chromatography (HPLC) system was employed as the control detection method
for the LC-MS-MS system. The two systems were compared in their specificity and accuracy.
Keywordsjasmonic acid-methyl jasmonate-extraction-purification-LC-MS-MS
Chinese Science Bulletin 04/2012; 55(21):2231-2235. · 1.32 Impact Factor