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ABSTRACT: ObjectiveHepatocellular carcinoma (HCC) is a complex disease which associates with both environmental and genetic factors. The purpose
of this study was to investigate whether the genetic polymorphisms of UDP-glucuronosyltransferase (UGT1A7), an important phase
II biotransformation enzyme, and X-ray repair cross-complementing group 1(XRCC1), a pivotal DNA-repair gene, were related
to the risk of HCC in Northeast China.
MethodsOne hundred and thirty six HCC patients and one hundred and thirty six frequency-matched controls were included in this hospital-based
case-control study. Genotypes of UGT1A7 and XRCC1 were determined using allele-specific polymerase chain reaction (AS-PCR)
and PCR-restriction fragment length polymorphism (RFLP), and for which the odds ratio (OR) with 95% confidence interval (95%
CI) were calculated.
ResultsThe proportion of UGT1A7 low enzymatic allele (*2 or *3) was higher in HCC patients than those in controls. The UGT1A7*1/*2
and *3/*3 genotypes were associated with higher HCC risk (OR=2.09, 95%CI: 1.10–3.97; OR=5.67, 95%CI: 1.76–18.30, respectively).
The XRCC1 codon 399 Arg/Gln genotype could also elevate HCC risk (OR=2.16, 95% CI 1.29–3.61). In addition to polymorphisms
of UGT1A7 and XRCC1, multivariate logistic regression analysis demonstrated that other significant independent factors associated
with HCC were HBV infection (OR=68.07, 95%CI: 28.03–165.26), HCV infection (OR=30.97, 95%CI: 8.06–118.94) and family history
of HCC (OR=10.62, 95%CI: 2.22–50.77).
ConclusionThe study shows that the polymorphisms of UGT1A7 and XRCC1 are associated with HCC risk. Determination of the polymorphisms
of UGT1A7 and XRCC1 may provide an important clue to preventive measure against HCC.
Key wordsHepatocellular carcinoma-(UDP)-glucuronosyltransferase 1A7(UGT1A7)-X-ray repair cross-complementing group 1(XRCC1)-Risk factors-Genetic polymorphism
Chinese Journal of Cancer Research 12/2010; 22(4):260-266. DOI:10.1007/s11670-010-0260-z