Yonghua Wang

Tongji Hospital, Wu-han-shih, Hubei, China

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Publications (5)5.87 Total impact

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    ABSTRACT: Bacillus Calmette-Guérin (BCG) has been used in the intravesical treatment of urothelial bladder cancer (UBC) for three decades. Despite its efficacy, intravesical BCG therapy is associated with some limitations such as side effects and BCG failure, which have inspired multiple ways to improve it. Recent advances have focused on recombinant BCG (rBCG) which provides a novel tactic for modification of BCG. To date, a number of rBCG strains have been developed and demonstrated to encourage efficacy and safety in preclinical and clinical studies. This review summarizes current rBCG strategies, concerns and future directions in UBC immunotherapy with an intention to encourage further research and eventually to inform clinical decisions.
    Expert Review of Anticancer Therapy 09/2014; · 3.22 Impact Factor
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    ABSTRACT: The aim of this study was to examine whether short hairpin RNA (shRNA) expressing lentiviral particles targeting B7-H1 infection could result in B7-H1 knockdown on dendritic cells (DCs) and to investigate whether B7-H1 silencing could augment the immune function of DCs and further elicit a more potent anti-tumor immune effect against bladder cancer cells in vitro.
    OncoTargets and Therapy 01/2014; 7:1389-96. · 2.07 Impact Factor
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    ABSTRACT: B7-H1, a recently described member of the B7 family of costimulatory molecules, is thought to be involved in tumor immune escape by inducing T-cell apoptosis. In order to investigate the relationship between B7-H1 and immune escape of bladder cancer, B7-H1 expression in 50 cases of bladder cancer was detected by using immunohistochemical method. Survival curves were constructed using the Kaplan-Meier method and independent prognostic factors were evaluated using the Cox regression model. Our results showed that the positive rate of B7-H1 immunostaining in normal bladder tissue and bladder cancer was 0 and 72% respectively. The expression of B7-H1 was strongly associated with the pathological grade, clinical stage and recurrence (P<0.05). The survival rate was significantly lower in patients with B7-H1 positive group than in those with B7-H1 negative group and multi-variable analysis revealed that B7-H1 could be regarded as an independent factor in evaluating the prognosis of bladder cancer. It is concluded that the expression of B7-H1 is strongly associated with neoplastic progression and prognosis of bladder cancer. The manipulation of B7-H1 may become a beneficial target for immunotherapy in human bladder cancer.
    Journal of Huazhong University of Science and Technology 02/2009; 29(1):77-9. · 0.58 Impact Factor
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    ABSTRACT: Objective The aim of this study was to study the expression and the clinical significance of B7-H1 on dendritic cells (DCs) in peripheral blood from patients with bladder cancer. Methods Peripheral blood mononuclear cell were disparted from 30 bladder cancer patients and 7 healthy controls by density gradient centrifugation and then co-cultured. The expression of B7-H1 on DCs were analyzed by flow cytometry. Results Expression of B7-H1 on DCs in bladder cancer was higher than healthy controls (P < 0.01). And the expression were strongly associated with the pathological grade and clinical stage of bladder cancer (P < 0.05). Conclusion The up-regulation of B7-H1 on DCs was strongly associated with neoplastic progression of bladder cancer. B7-H1/programmed death (PD)-1 signal pathway may also play an important role in immune escape of bladder cancer during initial phase of T cell immune response.
    The Chinese-German Journal of Clinical Oncology 12(6).
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    ABSTRACT: ObjectsThe aim of this study was to research the effect of grape seed procyanidin extract (GSPE) on cell apoptosis in human bladder cancer BIU87 cells and investigate its molecular mechanism. MethodsBIU87 cells were treated with different concentrations of GSPE and cultured for 24 h in vitro while untreated group as control, MTT[3-(4,5-dimethylthiazole-2-yl) -2, 5-diphenyltetrazolium bromide] assay, Hoechst 33258 stainning, flow cytometry, RT-PCR and Western blot were used to detect the apoptotic induction effect of GSPE on BIU87 cells. ResultsWe found that GSPE induced cell apoptosis in BIU87 cells by a dose-dependent manner. Semiquantitated RT-PCR and Western blot analyses indicated that GSPE increased the expression of caspase-3 and decreased the expression of survivin (P < 0.01). ConclusionGSPE induces apoptosis in BIU87 cells in vitro, and the effect maybe related with its down-regulation of survivin.
    The Chinese-German Journal of Clinical Oncology 8(7):420-425.