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ABSTRACT: Plant aquaporins are water-selected-channels in plants and are involved in seed germination, cell elongation, stoma movement,
fertilization and so on. Some plant aquaporins also play an important role in drought stress response. In this paper, the
gene encoding the Tamarix albiflonum Aquaporin (AQP) was amplified by 5′rapid amplification of cDNA end (RACE) on the basis of the sequence information obtained
from the expressed sequence tag of the subtractive hybridization library constructed under PEG6000 stress. The cDNA of the
T. albiflonum AQP gene is 1,043 bp long, encoding a protein of 287 amino acids with a predicted molecular mass of 30.9 kDa, has 6 transmembrane
regions, and possessing the major intrinsic protein (MIP) family signal consensus sequence SGXHXNPAVT and the higher plant
plasma membrane intrinsic protein (PIP) highly conservative sequence GGGANXXXXGY and TGI/TNPARSL /FGAA I/VI/VF/YN. A comparative
molecular analysis of the nucleotide sequence in National Center for Biotechnology Information (NCBI) databases showed that
it shared 95% homology with the gene of Arabidopsis thaliana (MIP-C), with a theoretical isoelectric point 8.84.
Frontiers of Forestry in China 03/2007; 2(2):217-221.
