M. Caccia

Publications (3)2.14 Total impact

• Article: Natural killer cells fate at the draining lymph nodes: a physical portrait of the biological contest

  L Sironi, M Caccia, N Gritti, T Gorletta, I Zanoni, C Salvetti, S Pozzi, S Freddi, S Daglio, C E Villa, M Collini, L D'Alfonso, F Granucci, G Chirico
  European Biophysics Journal 08/2011; 40(1):129. · 2.14 Impact Factor
• Chapter: In Vitro–In Vivo Fluctuation Spectroscopies

  M. Collini, L. D’Alfonso, M. Caccia, L. Sironi, M. Panzica, G. Chirico, I. Rivolta, B. Lettiero, G. Miserocchi
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  ABSTRACT: Fluorescence correlation spectroscopy (FCS) was first developed for biophysical studies in analogy with photon scattering correlation spectroscopy. Although it is mainly devoted to the study of freely diffusing particles, FCS is actually able to discern between different kinds of motions, such as diffusion, anomalous diffusion, or drift motions. The frontier application of FCS nowadays is in medical studies both within cells and on the cell membranes, and in the investigation of single molecules in solid matrices. In this field, FCS originated also image correlation spectroscopy methods. The whole field can be unified under the name of fluorescence fluctuation spectroscopy (FFS). We present here a short review of the theoretical bases of FFS under a unified vision and discuss some applications to the study of dynamics of nanoparticles in cells and to the investigation of the photodynamics of immobilized dyes.
  11/2010: pages 165-181;
• Source

  Available from: Maddalena Collini

  Article: Excited-State Lifetime Assay for Protein Detection on Gold Colloids−Fluorophore Complexes

  S. Freddi, L. D’Alfonso, M. Collini, M. Caccia, L. Sironi, G. Tallarida, S. Caprioli, G. Chirico
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  ABSTRACT: The interaction of the surface plasmons of gold nanoparticles (NP) a few nanometers in size with fluorophores can be used to engineer their fluorescence properties. This possibility can be exploited in principle to obtain nanodevices for protein−protein recognition. We studied different types of constructs based on gold NPs on which derivatives of fluorescein were bound. The interaction of this fluorophore with the gold surface plasmon resonances, mainly occurring through quenching, affects its excited-state lifetime that is measured by fluorescence burst analysis in standard solutions. The binding of proteins to the gold NPs through antigen−antibody recognition further modifies the dye excited-state lifetime. This change can therefore be used to measure the protein concentration. The data reported here indicate that one can measure the concentration of bovine serum albumine in solution with an apparent limit of detection of 5 ± 2 pM.
  02/2009;