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R Pal,
M Yang,
R Lin,
B N Johnson,
N Srivastava,
S Z Razzacki,
K J Chomistek,
D C Heldsinger,
R M Haque,
V M Ugaz,
P K Thwar,
Z Chen,
K Alfano,
M B Yim, M Krishnan,
A O Fuller,
R G Larson,
D T Burke,
M A Burns
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ABSTRACT: An integrated microfluidic device capable of performing a variety of genetic assays has been developed as a step towards building systems for widespread dissemination. The device integrates fluidic and thermal components such as heaters, temperature sensors, and addressable valves to control two nanoliter reactors in series followed by an electrophoretic separation. This combination of components is suitable for a variety of genetic analyses. As an example, we have successfully identified sequence-specific hemagglutinin A subtype for the A/LA/1/87 strain of influenza virus. The device uses a compact design and mass production technologies, making it an attractive platform for a variety of widely disseminated applications.
Lab on a Chip 11/2005; 5(10):1024-32. · 5.67 Impact Factor
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[show abstract]
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ABSTRACT: Over the past year there have been a number of recent advances in the fields of miniaturized reaction and separation systems, including the construction of fully integrated 'lab-on-a-chip' systems. Microreactors, which initially targeted DNA-based reactions such as the polymerase chain reaction, are now used in several other chemical and biochemical assays. Miniaturized separation columns are currently employed for analyzing a wide variety of samples including DNA, RNA, proteins and cells. Although significant advances have been made at the component level, the realization of an integrated analysis system still remains at the early stages of development.
Current Opinion in Biotechnology 03/2001; 12(1):92-8. · 7.71 Impact Factor
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ABSTRACT: Heat-transfer considerations significantly constrain design of
multiple reaction microdevices. We present a new methodology for design
and fabrication of multiple reaction systems using the concept of heat
integration. Heat integration is a design concept that strikes a balance
between complete thermal isolation of individual thermal operations and
power consumption in a multiple reaction device. It relies on the use of
steady-state temperature gradients developed in the substrate, by the
actuation of a single reaction chamber, to initiate several reactions at
progressively lower temperatures. The use of thermal gradients in this
manner eliminates power requirements to heat individual reactions,
active temperature control of “passive” reaction chambers
and power requirement for cooling. Complete thermal isolation on the
other hand, requires higher power for device cooling but, unlike the
case of heat integration, geometry of component placement is relatively
unconstrained
Micro Electro Mechanical Systems, 2001. MEMS 2001. The 14th IEEE International Conference on; 02/2001
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M A Burns,
B N Johnson,
S N Brahmasandra,
K Handique,
J R Webster, M Krishnan,
T S Sammarco,
P M Man,
D Jones,
D Heldsinger,
C H Mastrangelo,
D T Burke
[show abstract]
[hide abstract]
ABSTRACT: A device was developed that uses microfabricated fluidic channels, heaters, temperature sensors, and fluorescence detectors to analyze nanoliter-size DNA samples. The device is capable of measuring aqueous reagent and DNA-containing solutions, mixing the solutions together, amplifying or digesting the DNA to form discrete products, and separating and detecting those products. No external lenses, heaters, or mechanical pumps are necessary for complete sample processing and analysis. Because all of the components are made using conventional photolithographic production techniques, they operate as a single closed system. The components have the potential for assembly into complex, low-power, integrated analysis systems at low unit cost. The availability of portable, reliable instruments may facilitate the use of DNA analysis in applications such as rapid medical diagnostics and point-of-use agricultural testing.
Science 11/1998; 282(5388):484-7. · 31.20 Impact Factor
