Hyun-Suk Lee

Kyungpook National University, Daikyū, Daegu, South Korea

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Publications (5)0.99 Total impact

  • [show abstract] [hide abstract]
    ABSTRACT: Two genetic linkage maps, constructed by DH and RILs populations derived from the same parents, were carried out for the identification and comparison of QTLs controlling yield traits across different years in rice (Oryza sativa L.). A total of 194 SSR and STS markers were used in two maps, of which 114 markers were same. The distribution of Samgang allele was higher in RILs population than it in DH population. Comparing with DH population, RILs population has more lines with higher yield and wider phenotypic transgressive segression for yield traits. Although most of QTLs for the same trait were different in two populations across different years, 8 QTLs (including gwp11.1, spp5.1, spp10.1, spp11.2, ssr1.1, ssr11.1, tgw9.1 and tgw11.1) were detected over 2 yr. It is important to note that ppp10.1, spp10.1 and tgw9.1 were identified in two populations, while spp10.1 and tgw9.1 were simultaneity observed across different years. Epistatic effects were more important than additive effects for PPP, SPP, yield in DH population and TGW, yield in RILs population. Epistatic effects of DH and RILs populations were different on the same genetic background in the present study, which illuminated the QE interaction played an important role on epistatic effect. Identification and comparison of QTLs for yield traits in DH and RILs populations should provide various and more precise information. The QTLs identified in present study would be valuable in marker-assisted selection program for improving rice yield.
    Journal of Integrative Agriculture. 02/2013; 12(2):198–208.
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    ABSTRACT: This study was carried out to facilitate the functional analysis of rice genes. Some 297 insertion plants (1.7%) of the entire lines with the endogenous retrotransposon Tos17 were produced. Phenotypes of these plants in the S 2 generation were observed in the field according to different leaf types. Rolling leaf mutants showed thinner sclerenchymatous cells, defective arrangement of vascular bundles, and well-formed bulliform cells as compared to the parental cultivar. Two new copies of Tos17 were detected in the rolling leaf type. In the new leaf type, the copy number and activation of Tos12, 15 did not appear as 'Ilpum'. Flanking sequence tag (FST) analysis of Tos17 in the rolling leaf mutant indicated that new copies of Tos17 were transposed on chromosomes 11 and 12. Annotated homologues of the tagging genes on chromosome 11 were arabinoxylan rabinofuranohydrolase isoenzyme AXAH-I and II. The tagging gene in chromosome 12 was highly correlated with 6 kinds of genes including a transcript regulated factor and a rough sheath 2-like protein. This rolling leaf and flanking sequence data will stimulate the functional analysis of rice genes.
    American Journal of Plant Sciences. 01/2011; 2:56-62.
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    ABSTRACT: Rice lipid content as one of important ingredients of functional food and industrial products has become an entirely new target in the rice breeding programs worldwide. A genetic linkage map spanning 12 rice chromosomes with an average interval of 10.51 cM between markers was created using 172 DNA markers, which intended to elucidate genetic basis of lipid content in brown rice by QTL detection. Eight QTLs related to lipid content with LOD from 2.52 to 7.86 were mapped on chromosome1, 2, 3, 5, 6, 7 and 9 using a doubled haploid (DH) population from a cross of ‘Samgang/Nagdong’ with field experiments for five years. Two QTLs of qLC5.1 and qLC6.1 in the intervals 5014-5024 and 6011-RM19696 were repeatedly detected over four years at average LOD scores of 4.85 and 4.21, respectively. Five of eight QTLs tend to increase the lipid content from ‘Samgang’ alleles. Epistatic and environmental effects played important roles and explained 42.20% of phenotype variations. Three QTLs of qLC6.1, qLC7.1 and qLC9.1 collectively explained much than 27% of phenotype variations and increased 0.25% of lipid content and, showed much than 85% of selection efficiency for the lines with high lipid contents in the F7 population from a cross of ‘Samgang/Nagdong’. Thus it provides the sufficient possibility to realize QTLs pyramiding and to promote process of rice breeding. KeywordsLipid content–QTLs–MAS–Brown rice
    Genes & genomics 01/2010; 32(6):506-512. · 0.50 Impact Factor
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    ABSTRACT: Two genetic linkage maps based on doubled haploid (DH) and recombinant inbred lines (RILs) populations, derived from the same indica-japonica cross ‘Samgang × Nagdong’, were constructed to analyze the quantitative trait loci (QTLs) affecting agronomic traits in rice. The segregations of agronomic traits in RILs population showed larger variations than those in DH population. A total of 10 and 12 QTLs were identified on six chromosomes using DH population and seven chromosomes using RILs population, respectively. Three stable QTLs including pl9.1, ph1.1, and gwp11.1 were detected through different years. The percentages of phenotypic variation explained by individual QTLs ranged from 8 to 18% in the DH population and 9 to 33% in the RILs population. Twenty-three epistatic QTLs were identified in the DH population, while 21 epistatic QTLs were detected in the RILs population. Epistatic interactions played an important role in controlling the agronomic traits genetically. Four significant main-effect QTLs were involved in the digenic interactions. Significant interactions between QTLs and environments (QE) were identified in two populations. The QTLs affecting grain weight per panicle (GWP) were more sensitive to the environmental changes. The comparison and QTLs analysis between two populations across different years should help rice breeders to comprehend the genetic mechanisms of quantitative traits and improve breeding programs in marker-assisted selection (MAS). Key wordsagronomic traits–epistatic QTLs–QE interactions–QTLs
    13(4):235-241.
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    ABSTRACT: Seed shattering is an important factor causing loss of grain yield before and during rice harvest. In the present study, the quantitative trait loci regarding shattering scale, breaking tensile strength (BTS) and abscission layer (AL), the parameters evaluating seed shattering habit by hand gripping, a digital force gauge and observation on AL, respectively, were identified by using an doubled haploid line (DHL) population from a cross between a loose-shattering type Tongil variety, ‘Samgang’, and a moderately difficult shattering japonica variety, ‘Nagdong’. Eight QTLs consisted in four QTLs for shattering scale, two QTLs for AL, each one QTL for pulling and bending strength were detected on six chromosomes, respectively. Among them, Qss1 with flanking markers RM6696 and RM476 explained 31% of phenotype variation in shattering scale. Furthermore, two new QTLs controlling shattering habit, Qss5-2 and Qal5-1, were located on chromosome 5 at the interval 5028–5037 and 5021-RM289. They explained 10% and 12% of phenotype variations, respectively. A total of eleven digenic epistatic loci were identified for four parameters. The identification of QTLs affecting seed shattering habits is favorable to thoroughly dissect the genetic mechanism of the shattering habit and to apply for marker-assisted selection in rice breeding system of specific regions. KeywordsRice-Shattering scale-Pulling and bending strength-Abscission layer-QTLs
    Genes & genomics 32(2):173-180. · 0.50 Impact Factor

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0.99 Total Impact Points

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Institutions

  • 2010–2013
    • Kyungpook National University
      • School of Applied Biosciences
      Daikyū, Daegu, South Korea