L. Shan

Publications (2)1.42 Total impact

• Article: Heterologous expression of two Glycine max ω-3 fatty acid desaturases in Saccharomyces cerevisiae

  H. T. Zhang, Y. P. Bi, Z. J. Liu, L. Shan
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  ABSTRACT: α-Linolenic acid (ALA, C 18:3Δ9,12,15) has many important biological functions. ω-3 Fatty acid desaturase (FAD3), existing in cytosolic and plastidic compartments of higher plants, catalyzes linoleic acid (LA) desaturation to produce ALA. GmFAD3A-2 and GmFAD3C genes encoding cytosolic FAD3 from Qihuang 29 soybean were cloned and inserted into p416 vector and expressed in K601 yeast strain. Gas chromatography showed that the transformed yeast strains could produce ALA. The ALA accumulation levels for the strains transformed with GmFAD3A-2 or GmFAD3C genes were 0.77 ± 0.1 and 4.13 ± 0.4% of total fatty acids, respectively, while, as compared with that of the control, the contents of LA decreased from 14.34 ± 0.8 to 10.93 ± 0.0 and 7.85 ± 0.1%, respectively, implying that the GmFAD3C enzyme is more vigorous or stable, than GmFAD3A-2.
  Russian Journal of Plant Physiology 04/2012; 56(4):569-574. · 0.71 Impact Factor
• Article: Cloning of metallothionein genes from Arachis hypogaea and characterization of AhMT2a

  X. Q. Quan, L. Shan, Y. P. Bi
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  ABSTRACT: Seven cDNA sequences putatively encoding metallothionein (MT) proteins were isolated from peanut (Arachis hypogaea L.). We analyzed the sequences of the deduced amino acids and characterized one of these genes, AhMT2a. Northern blot analysis indicates that the accumulation of AhMT2a mRNA was found in stems, young roots, and young leaves, and AhMT2a mRNA levels were up-regulated by cadmium, copper, ABA, H2O2, and heat shock. The metal-binding properties of the recombinant protein expressed in E. coli was investigated, and the results show that bacteria harboring AhMT2a exhibited increased tolerance to cadmium, copper, and lead, and the metal accumulation was also higher than the controls. Taken together, our results suggest that the AhMT2a protein might function in both detoxification of heavy metals and scavenging of reactive oxygen species in peanut.
  Russian Journal of Plant Physiology 04/2012; 54(5):669-675. · 0.71 Impact Factor