Vijaya Koche

Publications (2)4.59 Total impact

• Article: Somatic embryogenesis in Buchanania lanzan spreng

  P. Sharma, Vijaya Koche, Afaque Quraishi, S. K. Mishra
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  ABSTRACT: We report a protocol for somatic embryogenesis and plantlet regeneration of Buchanania lanzan Spreng (Family—Anacardiaceae), which is a tropical fruit tree widely distributed in the dry forests of India. Calluses were initiated from immature zygotic embryos cultured on Murashige and Skoog (MS) medium supplemented with various combinations of 2,4-dichlorophenoxyacetic acid (2,4-D), 6-benzyladenine (BA) and/or 1-naphthaleneacetic acid (NAA). The highest frequency (60%) of somatic embryo induction was obtained in cultures grown on MS medium fortified with 4.53 μM 2,4-D, 5.32 μM NAA and 4.48 μM BA. The medium supplemented with 15 μM abscisic acid (ABA) was most effective for maturation and germination of somatic embryos. This is the first report on somatic embryogenesis in B. lanzan, which may be helpful for in vitro propagation, ex situ conservation and genetic manipulation of this species.
  In Vitro Cellular & Developmental Biology - Plant 08/2005; 41(5):645-647. · 1.50 Impact Factor
• Article: In vitro micropropagation from nodal segments of Cleistanthus collinus

  Afaque Quraishi, Vijaya Koche, S. K. Mishra
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  ABSTRACT: Cleistanthus collinus Benth. was micropropagated using nodal explants on MS medium supplemented with 2.2 M benzyladenine (BA). April to June was the best time for initiating shoot cultures. Shoot proliferation was enhanced when the BA concentration was lowered to 1.1 M. Rooting was achieved on half-strenth MS medium with 22.8 M indole-3-acetic acid for 7 days and continuous darkness for the first 72 h of the 7 days.
  Plant Cell Tissue and Organ Culture 03/1996; 45(1):87-91. · 3.09 Impact Factor