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ABSTRACT: The gynogenic response of a range of onion genotypes to flower bud culture was compared using a two-step culture system. Embryogenic
cultures and plantlets were produced from unpollinated ovules in whole flower bud explants 6 to 19 weeks after culture initiation.
Preconditioning stock plants significantly influenced gynogenic embryogenesis. A ten-fold increase in embryogenesis was obtained
when flower buds were cultured from stock plants maintained at 15 °C compared to 10 °C or the ambient temperature conditions
of a glasshouse (maximum-minimum of 25–12.7 °C). A total of 49 embryos was obtained from 2660 cultured flower buds and 45%
of plantlets were successfully acclimatised to glasshouse conditions. The majority of acclimatised plantlets were haploid
(68%) but spontaneous double haploid plants (23%) were obtained from three genotypes.
Plant Cell Tissue and Organ Culture 01/1999; 57(2):145-148. DOI:10.1023/A:1006312614874 · 2.13 Impact Factor