Long Li

Jiangsu University of Science and Technology, Chenkiang, Jiangsu Sheng, China

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Publications (2)5.82 Total impact

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    ABSTRACT: A full-length cDNA sequence encoding polygalacturonase-inhibiting protein (PGIP) from mulberry, which we designated MPGIP (GenBank accession no.: HM044383), was cloned based on mulberry expressed sequence tags (ESTs). Sequence analysis showed that the MPGIP is 1,274 base pairs (bp) in length, encoding 333 amino acids with a predicted molecular weight of 37.29 kDa and an isoelectric point of 7.25. The expression levels of MPGIP at different developmental stages in mulberry leaves and flowers and in different tissues were investigated. The results showed that MPGIP transcripts were most abundantly expressed in the young leaf, and the expression level of the mRNA could be increased significantly under salicylic acid (SA), abscissic acid (ABA), and NaCl stress conditions compared to the normal growth environment. And we also expressed the recombinant protein. This is to help understand a molecular basis for signal transduction mechanism underlying the stress response in mulberry.
    Plant Molecular Biology Reporter 01/2012; · 5.32 Impact Factor
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    ABSTRACT: A full-length cDNA sequence encoding CaM from mulberry, which we designated MCaM-3 (GenBank accession No: GQ303247), was cloned based on mulberry expressed sequence tags(ESTs). Sequence analysis showed that MCaM-3 is 951 base pairs in length, encoding 149 amino acids with a predicted molecular weight of 16.85 kD and an isoelectric point of 3.95. Online SMART analysis showed that the MCaM-3 protein has four EFh functional domains, which can bind calcium. The expression level of MCaM-3at different developmental stages in mulberry leaves and flowers and in different tissues was investigated. The results showed that MCaM-3 transcripts are most abundantly expressed in mature tissues, such as mature female flowers and climax leaves, and the expression level of the mRNA could be increased significantly under low temperature, drought, and salt stress conditions compared to the normal growth environment. This research will help us understand the resistance mechanism of functional genes in mulberry. Keywords Morus L. MCaM-3 gene–Molecular cloning–Gene expression
    Genes & genomics 01/2011; 33(2):97-103. · 0.50 Impact Factor

Publication Stats

5 Citations
5.82 Total Impact Points

Institutions

  • 2012
    • Jiangsu University of Science and Technology
      Chenkiang, Jiangsu Sheng, China
  • 2011
    • Chinese Academy of Agricultural Sciences
      • Institute of Sericultural Research (ISR)
      Peping, Beijing, China