[Show abstract][Hide abstract] ABSTRACT: Avian blood samples collected from remotely located farms may not always reach the laboratory for analysis immediately upon collection. This study investigated the changes that occur in the packed cell volume (PCV), haemoglobin concentration (HbC), red blood cell (RBC) counts, mean corpuscular values and white blood cell (WBC) counts of avian blood samples stored at refrigerator (4°C), room (average of 29°C) and incubator (37°C) temperatures across a storage period of 72 h. Blood samples for the study were collected from 12 adult chickens. All haematological determinations were carried out on the blood samples individually immediately upon collection to obtain the baseline value (BV) and thereafter at specific time intervals across the 72-h duration of storage (DOS). Results showed that for the samples stored at 4°C, there were no significant changes (p > 0.05) from the BV in the PCV, HbC, RBC counts, mean corpuscular haemoglobin (MCH) and mean corpuscular HbC (MCHC) all through the 72-h DOS, but the mean corpuscular volume (MCV) significantly increased (p < 0.01), while WBC counts significantly decreased (p < 0.01) from their BV after 30-h DOS. For the blood samples kept at 29°C, there were no significant changes (p > 0.05) from the BV in the HbC, RBC counts and MCH all through the 72-h DOS, but PCV and MCV significantly increased (p < 0.01) from their BV after 18 and 12-h DOS, respectively, while MCHC and WBC counts significantly decreased (p < 0.01) from their BV after 12 and 18-h DOS, respectively. For the samples kept at 37°C, there were significant changes (p < 0.01) from the BV in the MCHC after 9-h DOS, MCV after 12-h DOS, PCV and WBC counts after 18-h DOS, HbC and RBC counts after 48-h DOS and MCH after 60-h DOS. All changes in PCV, MCV and MCH were directly correlated with DOS, while the changes in HbC, RBC counts, MCHC and WBC counts were inversely correlated with the DOS. It was concluded that for avian blood samples stored at 4°C, reliable results (results not significantly different from the BV) can be obtained for the PCV, HbC, RBC counts, MCH and MCHC for up to 72-h DOS and for MCV and WBC counts for up to 30-h DOS; while for samples stored at 29°C, reliable results for HbC, RBC counts and MCH can be obtained for up to 72-h DOS and for MCV and MCHC for up to 12-h DOS but for PCV and WBC counts for up to 18-h DOS. Samples kept at 37°C can give reliable MCHC for up to 9-h DOS, MCV for up to 12-h DOS, PCV and WBC counts for up to 18-h DOS, HbC and RBC counts for up to 48-h DOS and MCH for up to 60-h DOS.
[Show abstract][Hide abstract] ABSTRACT: Reference values for the erythrocytic indices are vital for the diagnosis of anaemia and polycythemia and also for the assessment of efficacy of therapy instituted to correct these abnormalities. This study determined the reference values for the packed cell volume (PCV), haemoglobin concentration (HbC), red blood cell (RBC) counts, mean corpuscular volume (MCV), mean corpuscular haemoglobin (MCH) and mean corpuscular haemoglobin concentration (MCHC) of Anak 2000 broilers (A2B) at 14-day intervals for 84 days and Lohmann brown pullets (LBP) at 21-day intervals for 147 days. A total of 120 chickens were used for the study (60 A2B and 60 LBP), and all haematological determinations followed standard procedures. Results of the determinations on the A2B showed that PCV and HbC did not significantly vary (p > 0.05) with age and had a low correlation with age (r = −0.22 and r = 0.15, respectively), but RBC counts increased with and positively correlated with age (r = 0.78), while MCV and MCH decreased with and were strongly inversely correlated with age (r = −0.80 and r = −0.84, respectively), and MCHC did not follow a definite pattern. For the LBP, there were no significant variations (p > 0.05) associated with age in the PCV, RBC counts, MCV, MCH and MCHC up to 105 days of age; the significant variations (p < 0.05) obtained after day 105 were associated with pre-lay and egg-laying. The HbC of the LBP did not significantly vary (p > 0.05) all through the study. When compared with findings in similar studies as reported by other investigators, some of the values obtained in this study and the patterns of change in relation to age were in agreement while some others contrasted, probably because of differences between the strains and the climatic/environmental conditions under which the chickens were raised.