[Show abstract][Hide abstract] ABSTRACT: Glioblastoma is the most frequent, the most malignant and the best vascularised primary brain tumor. Substantial evidence
suggests that IGFBP-2 (insulin-like growth factor binding protein) may play a significant role in the development and progression
of various types of cancer, including the anaplastic progression of astroglial tumors. The correlation of IGFBP-2 and VEGF
over-expression in diffuse gliomas and strong IGFBP-2 immunostaining and co-expression with VEGF in the cells of pseudopalisading
necrosis suggests the induction of IGFBP-2 expression by hypoxia and/or possible involvement of its protein product in angiogenesis.
The aim of this study was to analyze the expression of IGFBP-2 and the relationship between the IGFBP-2 expression, extent
of bizarre angiogenesis and the presence of pseudopalisades. The prognostic impact of these variables was estimated, as well.
Our results revealed that most glioblastoma (75%) express IGFBP2 and that IGFBP2 expression was associated with the presence
of pseudopalisading necrosis as well as extensive bizarre angiogenesis. IGFBP-2 immunoreactivity in more than 5% of tumor
cells and the presence of pseudopalisading necrosis were prognostically significant in univariate analysis (p=0.034) while
in multivariate analysis only a patient’s age and the presence of pseudopalisades remained statistically significant (p=0.004).
The results of our study showed the association of IGFBP-2 expression in glioblastoma with pseudopalisades and bizarre angiogenesis
thus confirming on morphological grounds presumed induction of this gene under hypoxic conditions and its possible role in
[Show abstract][Hide abstract] ABSTRACT: Lipidized tumours of the central nervous system are very rare. Lipidization of tumour cells is a histological hallmark of
pleomorphic xanthoastrocytoma and cerebellar neurolipocytoma and has been described in some other primary neuroepithelial
tumours such as glioblastoma, cerebral primitive neuroectodermal tumour, central neurocytoma and ependymoma. However, a few
cases of lipidized low-grade glial tumours that could not be classified to the fore mentioned categories have been reported,
as well. We report a new case of such a tumour occupying the right temporal lobe in a 23-year old woman. Histologically, the
tumour was composed of GFAP positive glial cells with areas of complete cell lipidization. More than a two years after the
surgery, the patient is well and asymptomatic supporting presumed favourable clinical course of these rare tumours. The recommended
treatment plan for these presumably benignant tumours should be continued with radiographic surveillance after the gross total
[Show abstract][Hide abstract] ABSTRACT: Čupic H, KruČlin B, Belicza M. Epithelial hyperplastic lesions of the larynx in biopsy specimens. Acta Otolaryngol (Stockh) 1997; Suppl 527: 103-104.
Surgical biopsy files for the period 1991–92 were reviewed to determine the relative proportion and types of epithelial hyperplastic lesions of the larynx as well as sex and age distribution and correlation between clinical and histopathological diagnoses. In this period 203 laryngeal biopsies from 187 patients were analyzed. Epithelial hyperplastic lesions were found in 42 males and 9 females. There were 39 cases with keratosis and 12 cases showing keratosis with atypia. Correlation between clinical diagnoses and pathohistological findings was established in only 5 cases (9.8%), clinically diagnosed as leukoplakia; an additional 5 cases (9.8%) were clinically diagnosed as chronic laryngitis. The majority of clinical diagnoses in cases with epithelial hyperplastic lesions were laryngeal neoplasm (29, 56.9%) followed by laryngeal polyp in 9 cases (17.6%). We conclude that for correct diagnosis the biopsy should be performed in all patients with clinical symptoms showing laryngoscopic alterations that suggest a potentially malignant lesion.
[Show abstract][Hide abstract] ABSTRACT: The molecular mechanisms and candidate genes involved in development of meningiomas still need investigation and elucidation.
In the present study 33 meningiomas were analyzed regarding genetic changes of tumor suppressor gene Adenomatous polyposis
coli (APC), a component of the wnt signaling. Gene instability was tested by polymerase chain reaction/loss of heterozygosity
(LOH) using Restriction Fragment Length Polymorphism (RFLP) method. RFLP was performed by two genetic markers, Rsa I in APC’s
exon 11 and Msp I in its exon 15. The results of our analysis showed altogether 15 samples with LOH of the APC gene out of
32 heterozygous patients (47%). Seven patients had LOHs at both exons, while four LOHs were exclusive for exon 11 and four
for exon 15. The changes were distributed according to pathohistological grade as follows: 46% of meningothelial meningioma
showed LOH; 33% of fibrous; 75% of mixed (transitional); 75% of angiomatous, and one LOH was found in a single case of psammomatous
meningioma. None of the LOHs were found in atypical and anaplastic cases. Immunostaining showed that samples with LOHs were
accompanied with the absence of APC protein expression or presence of mutant APC proteins (χ2=13.81, df=2, P<0.001). We also showed that nuclear localization of β-catenin correlates to APC genetic changes (χ2=21.96, df=2, P<0.0001). The results of this investigation suggest that genetic changes of APC gene play a role in meningioma formation.
Journal of Neuro-Oncology 01/2008; 87(1):63-70. · 3.12 Impact Factor