G. S. Vengzhen

Publications (2)0.49 Total impact

• Article: Wheat plant DNA methylation pattern changes at chronic seed γ irradiation

  A. P. Kravets, T. A. Mousseau, A. V. Litvinchuk, Sh. Ostermiller, G. S. Vengzhen, D. M. Grodzinskiy
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  ABSTRACT: Alterations of DNA methylation patterns of two wheat sorts Al’batros odesskii and Donetskaya-48 whose seeds were irradiated with a low dose rate (3 × 10−7 Gy/s) for 4 months have been studied. Six restriction endonucleases were used in the experiments. Primary distinction in DNA methylation patterns of the studied sorts has been demonstrated. Chronic irradiation resulted in an increase of the methylation level on the recognion site for GluI and Sou3AI and in a decrease of this index in recognion sites for endonucleases GlaI and HpaII. The alterations of the methylation level in recognition sites for restrictases MboI and MspI were not found. The considerable increase of chromosome aberration level at the same dose of chronic irradiation has been shown. The role of DNA methylation pattern changes in development of irradiation damage and organism protective reactions is discussed.
  Cytology and Genetics 04/2012; 44(5):276-279. · 0.25 Impact Factor
• Article: Dynamics of hybrid dysgenesis frequency in Drosophila melanogaster in controlled terms of protracted radiation exposure

  A. P. Kravets, T. A. Musse, Z. A. Omel’chenko, G. S. Vengzhen
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  ABSTRACT: The dynamics of hybrid dysgenesis frequency in F 1 descendants of two lines of Drosophila melanogaster (lines Santon-s and radius incompletus (ri) brining P-mobile element), which was maintained for 20 generations were under the conditions of chronic radiation with three dose rate (1.2 × 10−8; 0.3 × 10−8; 0.12 × 10−8 Gy/s), have been studied. Gradual changes have been shown in the dose dependences of the hybrid dysgenesis frequency for F 1 descendants of every 20 generations from parents exposed to radiation. The complicated dynamics of the transitional process of the appearance of gonadal dysgenesis depending on the duration and dose rate of irradiation in ancestors, has been found. The cumulative effect of the prolonged irradiation appears in the form of adaptation at the lowest dose rate of irradiation and at exhaustion at the highest used dose rate. Problem of the transitional process peculiarities, including the hierarchy of protective and adaptive reactions under the conditions of the chronic irradiation has been discussed.
  Cytology and Genetics 04/2012; 44(3):144-148. · 0.25 Impact Factor