[Show abstract][Hide abstract] ABSTRACT: species level. Observed number of alleles was 1.9093 ± 0.2875 and effective number of alleles was 1.4048 ± 0.3342. Nei's gene diversity and Shannon's Information index were 0.2494 ± 0.1657 and 0.3903 ± 0.2186, respectively. Nei's genetic distance among 22 populations ranged from 0.0996 to 0.6952. Genetic identity among populations ranged from 0.4990 to 0.9052, averaging 0.7387. Unweighted pair group method with arithmetic mean (UPGMA) cluster analysis based on Nei's genetic distance indicated that most populations were positioned into the relevant area. The above-mentioned results suggested the level of genetic variation of P. paniceum paniceum (Lam.) Hack. was low. The high score of PPB might be caused by low frequent polymorphism distributed in different populations. A significant correlation between genetic and geographic distances of populations was revealed based on Mantel test.
[Show abstract][Hide abstract] ABSTRACT: Using degenerate primers and RT-PCR, RACE techniques, a 1491 bp cDNA segment of stearoyl-acyl carrier protein desaturase (SAD)
is cloned from developing seeds of Jatropha curcas L. The segment contains a 1191 bp of complete open reading frame (ORF). Analysis in the BLAST on NCBl shows that Jatropha curcas SAD (JSAD) gene encodes a protein precursor composed of a signal peptide of 33 amino acids and a mature peptide of 363 amino
acids. The homological analysis shows that JSAD has high level of homology both in nucleotide sequence and in amino acid sequence
to other plants SADs. The nucleotide and peptide identity of JSAD to Ricinus communis SAD (RSAD) is up to 89% and 96.2% respectively. Molecular modeling of JSAD indicates that its three-dimensional structure
strongly resembled the crystal structure of RSAD.
Journal of Shanghai University (English Edition) 04/2007; 11(2):182-188. DOI:10.1007/s11741-007-0218-7