RongNa Ma

Publications (2)2.4 Total impact

• Article: Fabrication of the DNA/poly(3-methylthiophene) composite film modified electrode and its application for the study on the voltammetric behavior and determination of 8-hydroxy-2′-deoxyguanosine

  YanHuai Wang, Jing Li, Yan Liu, RongNa Ma, WenLi Jia, Hui Cui, HuaiSheng Wang
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  ABSTRACT: A composite film of DNA/poly(3-methylthiophene) (P3MT) modified glassy carbon electrode (GCE) has been fabricated by electro-deposition method. P3MT film was first electropolymerized at the GCE and the DNA layer was then immobilized on the P3MT layer by electrochemical method. The voltammetric behavior of 8-hydroxy-2′-deoxyguanosine (8-OH-dG) at the composite film modified electrode was studied. The effects of scan rates, pH and the interference of uric acid (UA) on the voltammetric behavior and detection of 8-OH-dG were also discussed. The experimental results suggest that the electrochemical behavior of 8-OH-dG at the composite film modified electrode was greatly improved due to the combination of the advantages of P3MT and DNA. In 0.1 M pH 7.0 phosphate buffer solution (PBS), the anodic peak currents of 8-OH-dG were linear with the 8-OH-dG concentration in two intervals, viz. 0.28–4.2 µM and 4.2–19.6 µM. The detection limit of 56 nM 8-OH-dG could be estimated (S/N = 3). This proposed composite film modified electrode shows excellent reproducibility and stability. It may have the potential application for the detection of 8-OH-dG in human urine.
  Science in China Series B Chemistry 04/2012; 52(11):2006-2012. · 1.20 Impact Factor
• Article: Direct electrochemistry of glucose oxidase on the hydroxyapatite/Nafion composite film modified electrode and its application for glucose biosensing

  RongNa Ma, Bin Wang, Yan Liu, Jing Li, Qian Zhao, GuoTao Wang, WenLi Jia, HuaiSheng Wang
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  ABSTRACT: A novel glucose biosensor was constructed by immobilizing the glucose oxidase (GOD) on a hydroxyapatite (HAp)/Nafion composite film modified glassy carbon electrode (GCE) and applied to the highly selective and sensitive determination of glucose. With the cooperation of HAp and Nafion, the composite film played an important role in enhancing the stability and sensitivity of the biosensor. The results demonstrate that the GOD adsorbed onto the HAp/Nafion composite film exhibits a pair of well-defined nearly reversible redox peaks and fine catalysis to the oxidation of glucose companied with the consumption of dissolved oxygen. On the basis of the decrease of the reduction current of dissolved oxygen at the applied potential of −0.80 V (vs. SCE) upon the addition of glucose, the concentration of glucose could be detected sensitively and selectively. The decreased reduction current was linear with the concentration of glucose in the range of 0.12–2.16 mM. The detection limit and sensitivity were 0.02 mM (S/N = 3) and 6.75 mA·M−1, respectively. All the results demonstrate that HAp/Nafion composite film provides a novel and efficient platform for the immobilization of enzymes and realizes the direct electrochemistry. The composite materials should have potential applications in the fabrication of third-generation biosensors.
  Science in China Series B Chemistry 04/2012; 52(11):2013-2019. · 1.20 Impact Factor