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ABSTRACT: A simple high-performance liquid chromatographic method was developed for determining five major components of teicoplanin,
designated A2–1, A2–2, A2–3, A2–4 and A2–5, in human plasma. Using piperacillin sodium as internal standard, teicoplanin in
plasma samples was extracted by coextractive cleanup procedure. The extracts were injected into a Nova-Pak C18 column maintained at ambient temperature. The mobile phase consisted of acetonitrile–0.1% trifluoroacetic acid (27:73, pH=2.2),
at a flow rate of 1.0mLmin−1. The analytes were detected at the UV wavelength of 218nm. The method was found to be linear over the concentration range
of 2.5–50mgL−1 for teicoplanin (r=0.9993±0.0038), which covered the clinically expected trough plasma levels. The percentage error of the analytical method
was below 9%. The intra- and inter-day reproducibility was adequate with coefficients of variation less than 7%. The chromatographic
running time was 11min. Thus, the method can be effectively applied to measure teicoplanin concentrations in clinical samples.
Chromatographia 12/2006; 65(1):9-12. · 1.20 Impact Factor
