Guoshi Qian

Publications (2)4.88 Total impact

• Article: Effect of cutinase on the degradation of cotton seed coat in bio-scouring

  Hejing Yan, Zhaozhe Hua, Guoshi Qian, Miao Wang, Guocheng Du, Jian Chen
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  ABSTRACT: In this paper the effect of cutinase on the degradation of cotton seed coat is analyzed. Fourier transform infrared (FT-IR) microspectroscopy was applied to study the changes of chemical compositions in cotton seed coat epidermal layer and gas chromatography/mass spectrometry (GC/MS) was used to analyse cutinase depolymerization of cotton seed coat. Based on these arguments the ability of cutinase to degrade aliphatic components in cotton seed coat was verified. Positive effect of cutinase on degradation of cotton seed coat was observed with the combination of alkaline pectinase or xylanase. The removal of aliphatic components by cutinase enables other enzymes to penetrate into the inner of cotton seed coat. Cutinase can potentially improve the degradation of cotton seed coat during cotton fabric bio-scouring process.
  Biotechnology and Bioprocess Engineering 04/2012; 14(3):354-360. · 1.28 Impact Factor
• Article: Analysis of the chemical composition of cotton seed coat by Fourier-transform infrared (FT-IR) microspectroscopy

  Hejing Yan, Zhaozhe Hua, Guoshi Qian, Miao Wang, Guocheng Du, Jian Chen
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  ABSTRACT: To improve knowledge on the biodegradation of cotton seed coat fragments in bioscouring, in this study Fourier-transform infrared (FT-IR) microspectroscopy was performed to analyze the composition of cotton seed coat. Microscope observation showed that cotton seed coat has a five-layer structure: epidermal layer, outer pigment layer, colorless layer, palisade layer, and inner pigment layer. Analysis of the FT-IR spectra from each layer shows that cutin, wax, cellulose, and pectin are the main components of the epidermal layer, while pectin and hemicellulose are the main constituents of the palisade layer, as well as aromatic and polyphenol, which are commonly considered as lignins. The main component of the outer and inner pigment layers is lignin. The results suggest that cellulase, pectinase, xylanase, and lignin oxidase are suitable for degradation of cotton seed coat. In addition, cutinase might be very promising in improving the enzymatic degradation of cotton seed coat.
  Cellulose 04/2012; 16(6):1099-1107. · 3.60 Impact Factor