ABSTRACT: ObjectiveWe studied the effects of Wortmannin (WM) on the proliferation and apoptosis of leukemia cells, and explore the possible mechanisms.
MethodsThe human myeloid leukemia cell line K562 was treated with different concentrations of WM, and then detected the activity
of the cell proliferation by MTT assay, comet tail formation of cell DNA damage phenomenon by single cell gel electrophoresis,
cell apoptosis by Annexin V-FITC/PI double staining and the expression levels of total Akt, phoshorylated Akt, NF-κB and protein
in K562 cell by Western blotting, RT-PCR test before and after WM.
ResultsWM inhibited cell proliferation of K562 in a concentration-dependent manner, with IC50 value for 24 h being 25 nmol/L. WM induced apoptosis of K562 cells in a concentration-dependent manner, and could induce
the breakage of DNA strand of K562 cell. The rate of DNA tail and the tail length of experimental groups were significantly
higher than that of control group. WM may inhibit the expression of phosphorylated Akt and NF-KB protein in a dose-dependent
manner in both the protein and gene levels, but no significant effect on total Akt protein.
ConclusionWM inhibited cell proliferation and induced apoptosis in K562 and concentration-dependent manner. The possible mechanism may
be involved in the regulation of survival signaling pathway, such as PI3K/Akt/Nκ-KB.
Key wordsWortmannin-K562 cell-p-AKT-NF-KB
The Chinese-German Journal of Clinical Oncology 04/2012; 9(12):734-738.