Andrzej Denys

University of Lodz, Łódź, Łódź Voivodeship, Poland

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Publications (6)5 Total impact

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    ABSTRACT: Surface proteins play an important role in the pathogenesis of enterococcal infections. Some of them are candidates for a vaccine, e.g., the frequency of endocarditis in rats vaccinated with Ace protein was 75 % as 12 opposed to 100 % in those who weren’t. However, there are other components of enterococcal cells, such as Epa antigens or internalin-like proteins, which may be used in the prophylaxis of infections caused by them. However, also other virulence factors and resistance to antibiotics are important during enterococcal infection. Therefore, the relevance of ace, epa, elrA, other virulence genes, as well as resistance to antibiotics was investigated. 161 Enterococcus faecalis strains isolated from teaching hospitals in Lodz, cultured according to standard microbiological methods, were investigated for the presence of genes encoding surface proteins by PCR. Results were analyzed with χ2 test. The elrA gene was found in all clinical and environmental strains, the ace gene was also widespread among E. faecalis (96.9 %). Both tested epa genes were found in the majority of isolates (83.25 %). There was correlation between the presence of esp and ace genes (p = 0.046) as well as between epa and agg genes (p = 0.0094; χ2 test). The presence of the genes encoding surface proteins investigated in our study in the great majority of isolates implies that they would appear to be required during E. faecalis infection. Therefore, they could be excellent targets in therapy of enterococcal infections or, as some studies show, candidates for vaccines.
    Indian Journal of Microbiology 12/2012; 52(4). DOI:10.1007/s12088-012-0285-8 · 0.90 Impact Factor
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    ABSTRACT: Aims: The spread of bacterial antibiotic resistance among clinical strains is a growing problem nowadays. Resistance in Enterococcus spp. strains has merited special concern of late, as they have become significant nosocomial pathogens. Hence, the use of compounds such as silver nanoparticles (AgNPs), displaying various mechanisms of action, is a growing new approach against virulent, drug-resistant infections. Therefore, the activity of AgNPs obtained by a physical method (microexplosion method) was tested against 30 clinical and environmental Enterococcus spp. strains presenting diverse virulence and resistance patterns. Results: The minimal inhibitory concentration for all the species ranged from 0.39 to 3.125 μg/ml. No statistical differences (p=0.26) were found between resistance to AgNPs and resistance to antibiotics, suggesting high activity of AgNPs even against multidrug-resistant strains. Conclusions: AgNPs obtained by this method seem to be a promising alternative to chemotherapeutics in the treatment or prevention of enterococcal infections.
    Microbial drug resistance (Larchmont, N.Y.) 10/2012; 19(1). DOI:10.1089/mdr.2012.0052 · 2.49 Impact Factor
  • Indian Journal of Microbiology 06/2012; · 0.90 Impact Factor
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    ABSTRACT: The aim of this work was to investigate the antimicrobial properties of thyme essential oil against clinical multiresistant strains of Acinetobacter spp. The antibacterial activity of oil was tested against standard and clinical bacterial strains of Acinetobacter genus. The agar diffusion method was used to check the inhibition of microbial growth at various concentrations of the oil from Thymus vulgaris. Susceptibility testing to antibiotics and chemotherapeutics was prepared using the disc-diffusion method. Identification of bacterial strains was carried out with the Vitek system and confirmed by PCR for Acinetobacter baumanii gyrB gene. The results of experiments showed that the oil from T. vulgaris exhibited an extremely strong activity against all of the clinical strains of Acinetobacter. Thyme oil demonstrated a very good efficacy against multiresistant strains of tested bacteria. Essential oils seems to be an excellent alternative for synthetic preparations and that is reason for an extensive assessment of their antimicrobial activity. KeywordsAntibacterial activity–Thyme oil–Minimal inhibitory concentration–Multiresistant strains
    Central European Journal of Biology 06/2011; 6(3):405-413. DOI:10.2478/s11535-011-0008-x · 0.71 Impact Factor
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    ABSTRACT: The aim of this study was to evaluate occurrence of virulence genes among Enterococcus faecalis strains isolated from two hospitals in Łódź, during 2005-2006. The second goal was to determine possible relationship between presence of those genes, resistance to ampicillin and sources of isolation. Enterococcal strains were identified to the species by PCR with ddl primers. All 161 isolates were tested for the presence of aggregation substance gene (agg), cytolisine genes (cyl-L, cyl-S), esp protein gene, gelatinase gene (gelE), serine protease gene (sprE). Susceptibility to ampicillin was tested by microdillution method. Both cyl-L and cyl-S genes were found in 52,2% of strains, agg gene was present in 62,73% isolates, esp gene--in 71.2%. Most frequently found genes were gelE (85.1%) and sprE (82.6%). The presence of esp gene in isolates resistant to ampicillin was statistically higher than in susceptible strains, what might suggest appearance of epidemic strains. Besides, strains possessing both, cyl-L/S and esp genes, were found to be statistically more often isolated strains than those possessing only single genes.
    Medycyna doświadczalna i mikrobiologia 01/2009; 61(2):125-32.
  • Monika Eliza łysakowska · Andrzej Denys
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    ABSTRACT: The aim of this study was to evaluate antibiotic susceptibility of Enterococcus sp. strains isolated from two hospitals in łódź, during 2005-2006. The second goal was to determine possible transmission of these strains within hospital wards by using melting profile PCR. Enterococcal strains were identified to species according standard microbiological methods. There was isolated 159 strains of E. faecalis, 51 strains of E. faecium and two E. avium, 1 E. durans. Susceptibility to antimicrobial agents was tested by disc diffusion method. None of these strains was resistant to vancomycin, teicoplanin or linezolid. There was high percentage of strains resistant to aminoglicosides, 22% of E. faecalis strains, and 54.9% of E. faecium strains, respectively. Additionally it was shown that 11.7% of E. faecium is resistant to chinuprisin-dalfopristin. The strains with similar pattern of resistance to antibiotics and fenotypic characteristics were genotyped by mpPCR. This technique was useful to confirm relatedness of bacterial strains suspected of being spread within hospital wards.
    Medycyna doświadczalna i mikrobiologia 01/2008; 60(1):19-26.