Publications (4)3.48 Total impact
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ABSTRACT: Objective: To establish a fluorescent multiplex amplification of 16srRNA and ND4 genes in mitochondrial DNA in order to provide a method for forensic species identification. Methods: The mitochondrial DNA sequences of human and different kinds of animals, including monkey, pig, dog, fish, rat, guinea pig, rabbit, horse, eel, frog, chicken, duck, goat and cow, were obtained from GenBank. The difference of mitochondrial DNA sequences between human and animals were analyzed with software. With software Primer 5,two pairs of primers were designed for 16srRNA and ND4 genes chosen from mtDNA sequence. One primer of each pair was labeled with 6-FAM in 5′ end. Multiplex amplification was carried out with the primers. The amplified products were analyzed by ABIPRISM 310 Genetic Analyzer. DNA samples from Human and 14 kinds of different animals were analyzed. Results: The fluorescent multiplex amplification of 16srRNA and ND4 genes was successfully carried out. The amplified products of human DNA had two peaks, one of which was human-specific 110-bp-length fragment and another was 149-bp-length fragment. The products of animals DNA had only one peak, the size of which was 149bp. Therefore, the 149-bp-length fragment belonged to both Human and animals. Conclusion: A system of fluorescent multiplex amplification of mitochondrial DNA for forensic species identification was successfully established, and it could distinguish Human samples from familiar animal samples.Chinese Journal of Forensic Medicine 01/2006; 21(5):272-274.
- Journal of Forensic Sciences 08/2005; 50(4):958. DOI:10.1520/JFS2005007 · 1.16 Impact Factor
- Journal of Forensic Sciences 06/2005; 50(3):713. DOI:10.1520/JFS2004432 · 1.16 Impact Factor
- Journal of Forensic Sciences 04/2005; 50(2):476. DOI:10.1520/JFS2004414 · 1.16 Impact Factor