ABSTRACT: ObjectiveTo investigate the available parameters in gynecological screening for cervical lesions by liquid-based cytology technology
(ThinPrep Cytology Test, TCT) and The Bethesda System (TBS), also with computer image analysis.
MethodsWith application of the image analysis system, all grades of cervical lesion cells were detected quantitatively and sorted
in atypical squamous cells of undetermined significance (ASCUS), atypical squamous cells-cannot exclude HSIL (ASC-H), low-grade
squamous intraepithelial lesion (LSIL), high-grade squamous intraepithelial lesion (HSIL) and cervical squamous cell carcinoma
(SCC) with the mean optical density (MOD), average grey (AG), positive units (PU), and nucleus to cytoplasmic ratio (N: C).
Differences between each group of cells were compared and analyzed statistically.
ResultsApart from four stereologic parameters in LSIL and HSIL groups there were no differences among them, in the other groups,
there was statistically significant in differences between MOD, AG and PU values. Differences between them in the ratio of
nucleus to cytoplasm were highly statistically significant.
ConclusionStereological indexes may serve as a screening tool for cervical lesions. The image analysis system is expected to become
a new means of cytological assisted diagnosis.
Key Wordscell biology-uterine cervical neoplasms-TBS-image analysis-computer-assisted
Clinical Oncology and Cancer Research 04/2012; 7(5):299-302.