Chang-you Gao

Publications (2)1.33 Total impact

• Article: A hybrid scaffold of poly(lactide-co-glycolide) sponge filled with fibrin gel for cartilage tissue engineering

  Wei Wang, Dan Li, Mei-cong Wang, Yang-lin Li, Chang-you Gao
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  ABSTRACT: The poly(lactide-co-glycolide) (PLGA) sponge fabricated by a gelatin porogen leaching method was filled with fibrin gel to obtain a hybrid scaffold for chondrocytes culture in vitro. The fibrin gel evenly distributed in the hybrid scaffold with visible fibrinogen fibers after drying. In vitro culture it was found that in the hybrid scaffold the chondrocytes distributed more evenly and kept a round morphology as that in the normal cartilage. Although the chondrocytes seeded in the control PLGA sponges showed similar proliferation behavior with that in the hybrid scaffolds, they were remarkably elongated, forming a fibroblast-like morphology. Moreover, a larger amount of glycosaminoglycans was secreted in the hybrid scaffolds than that in the PLGA sponges after in vitro culture of chondrocytes for 4 weeks. The results suggest that the fibrin/PLGA hybrid scaffold may be favorably applied for cartilage tissue engineering. KeywordsCartilage tissue engineering–PLGA sponge–Fibrin gel–Chondrocytes
  Chinese Journal of Polymer Science 05/2012; 29(2):233-240. · 0.92 Impact Factor
• Source

  Available from: zju.edu.cn

  Article: Fabrication of microcapsule arrays on chemically patterned surfaces via covalent linking

  Jie Yang, Chang-you Gao
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  ABSTRACT: A method for fabricating arrays of microcapsules covalently immobilized onto chemically patterned substrates was developed. The core-shell microparticles with poly(allylamine hydrochloride) (PAH) as the outermost layer were obtained by layer-by-layer (LbL) assembly, which were further treated with glutaraldehyde to endow the particles with abundant aldehyde groups on their surfaces. The particles were then covalently coupled to the chemically patterned regions with amino groups created by microcontact printing (μCP). After dissolution of the core particles, arrays of the hollow microcapsules with unchanged structures were obtained. These arrays could stand rigorous environmental conditions of higher ionic strength, and lower and higher pH values. Thus, the technique could be possibly applied to exploiting chips of microcontainers or microreactors in sensing technology.
  Journal of Zhejiang University - Science A: Applied Physics & Engineering 12/2008; 10(1):114-120. · 0.41 Impact Factor