Publications (2)1.2 Total impact
Article: Separation of Delphinidin-3-O-sambubioside, Cyanidin-3-O-sambubioside and p-Coumaric Acid from Cranberry by CCC Followed by prep-HPLC Using Robotic CCC Solvent System Selection[show abstract] [hide abstract]
ABSTRACT: High-performance counter-current chromatography has been used for the separation of delphinidin-3-O-sambubioside, cyanidin-3-O-sambubioside and p-coumaric acid from crude extract of cranberry. The separation was performed with a two-phase solvent system composed of butanol/0.05% aqueous trifluoroacetic acid/methanol at a volume ratio of 4:5:1. The two-phase solvent system was selected following the determination of partition coefficients (K) in a range of solvent systems using a robotic solvent system selection method. Analytical scale CCC confirmed that this phase system separated the components from a crude cranberry extract (40mg scale) with acceptable purities. Preparative CCC of 400mg of crude yielded 4.2mg of p-coumaric acid at a purity of over 98%, 3.6mg of delphinidin-3-O-sambubioside at a purity of over 97% and 4.5mg of cyanidin-3-O-sambubioside at a purity of 73%, which was further purified by preparative high-performance liquid chromatography to yield 3mg cyanidin-3-O-sambubioside at 95% purity. The identification of delphinidin-3-O-sambubioside, cyanidin-3-O-sambubioside and p-coumaric acid was performed by ESI-MS, 1H-NMR and 13C-NMR spectra. KeywordsHigh-performance counter-current chromatography–Cranberry–Delphinidin-3-O-sambubioside–Cyanidin-3-O-sambubioside– p-Coumaric acid–Preparative high-performance liquid chromatographyChromatographia 04/2012; 74(5):367-373. · 1.20 Impact Factor
Article: Origin of long chain alkylbenzenes in environmental and geological samples and their analysis with different chromatographic techniques[show abstract] [hide abstract]
ABSTRACT: Linear long chain alkylbenzenes (LCABs) are present as an impurity in linear alkylbenzene sulfonates (LAS), widely used surfactants. These hydrophobic linear LCABs deposit in sediments and are a constant source of pollution in the environment. They have also been identified from geological samples. It is thought that they have no geological origin but present in geological samples as an impurity during washing and cleaning the laboratory equipments. The range of LCABs in geological samples is wider than that used in surfactants, which indicates that both have different origins. Long chain fatty acids and alcohols are commonly found in bacteria and algae. They can be converted into LCABs. Sulfur, lignin and karogen can play an important role for such kind of conversions. Such reactions involve cyclization, aromatization and decarboxylation. The reactions of these alcohols and fatty acids with benzene in the presence of clay are another source of LCABs. In this paper, the origins of long chain alkylbenzenes in geological and environmental samples have been discussed. Their methods of analysis have also been described. GC-MS is widely used for the analysis of LCABs in geological and environmental samples. Multidimensional gas chromatography has also been used successfully. Keywordslong chain alkylbenzenes–environmental samples–geological samples–origin–review04/2012; 1(3):288-307.