ABSTRACT: A simple, sensitive and rapid method for the analysis of jasminoidin in rabbit plasma by liquid chromatography coupled to
tandem mass spectrometry was developed. Detection was by positive ion electrospray ionization in multiple reactions monitoring
mode. The method included a chromatographic run of 5.0min using a C18 analytical column and the calibration curve was linear over the concentration range of 0.5–2,000ngmL−1 with a correlation coefficient R of 0.998 or better. The intra- and inter-day precision ranged from 3.4 to 5.6% and 4.3 to 8.2%. The intra- and inter-day
assay accuracy was between −7.4 and 8.6%. The method was successfully applied for the pharmacokinetic study on jasminoidin
in rabbit after a single dose oral administration of Gardenia jasminoides Ellis (Gardenia) and Gardenia coupling Coptis chinensis Franch (Coptidis) extracts.
KeywordsColumn liquid chromatography-mass spectrometry-Pharmacokinetic study-
Gardenia jasminoides Ellis
Chromatographia 05/2012; 71(11):1031-1037. · 1.20 Impact Factor