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Publications (3)1.01 Total impact

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    ABSTRACT: The monoclonal dominant malignant T cells in mycosis fungoides (MF) carry identical TCR gamma rearrangements. Their detection is a useful diagnostic tool. Thus, in routine diagnosis we investigated the occurrence of monoclonal T cells in skin biopsy samples of MF-patients by TCR gamma-PCR, followed by temperature gradient gel electrophoresis (TGGE). In 188 out of 208 MF patients, at least one skin sample with sufficient DNA quality for PCR analysis was obtained. Applying a consensus PCR for the TCR gamma genes V gamma I and J gamma 1/2, we detected monoclonal T cells in 122 cases (65%). In the remaining 66 cases, we performed two multiplex-PCRs, covering rearrangements of the other TCR gamma genes. Here we found in 11 cases (6%) predominant clonal rearrangements of V gamma II-IV and J gamma 1/2 and in 2 (1%) those of V gamma I-IV and J gamma P 1/2. In patients with MF, detecting rearrangements of only V gamma I and J gamma 1/2 is sufficient for PCR screening analysis. In 53 of the patients (28%) the applied methods revealed no monoclonal T cells. This may be due to a low cell number, oligoclonal nature, chromosomal abberations or remaining of TCR in germline configuration.
    Der Hautarzt 09/1998; 49(8):641-5. · 0.50 Impact Factor
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    ABSTRACT: Maligne, klonal expandierte T-Zellen bei der Mykosis fungoides (MF) besitzen identische T-Zellrezeptor- (TCR-)Rearrangements, deren Nachweis für die Diagnosestellung sehr hilfreich ist. Wir untersuchten routinemäßig Hautbioptate von Patienten mit verschiedenen Verdachtsdiagnosen mittel TCRγ-PCR und Temperaturgradienten-Gelelektrophorese (TGGE). In 188 von 208 MF-Patienten konnte amplifizierbare DNA aus mindestens einer Hautprobe gewonnen werden. Mittels Konsensus-PCR für die Gensegmente VγI und Jγ 1/2 ließen sich klonale T-Zellen in 122 dieser 188 Fälle (65%) nachweisen. Für die Proben der verbleibenden 66 Patienten erfolgten jeweils 2 Multiplex-PCR für Rearrangements anderer TCRγ-Gene. Klonale Kombinationen von VγII,III,IV mit Jγ1,2 ergaben sich bei 11 Patienten, von VγI,II,III,IV mit JγP1,P2 nur bei 2 Patienten. Als Klonalitätsscreening genügt folglich eine PCR für Rearrangements von VγI und Jγ 1/2. Bei 53 Patienten (28%) konnten wir mit unseren Methoden keine klonal dominierenden T-Zellen finden. Dies läßt sich mit zu geringen Zellzahlen, Oligoklonalität, chromosomalen Abberationen oder TCR in Keimbahnkonfiguration erklären. The monoclonal dominant malignant T cells in mycosis fungoides (MF) carry identical TCRγ rearrangements. Their detection is a useful diagnostic tool. Thus, in routine diagnosis we investigated the occurrence of monoclonal T cells in skin biopsy samples of MF-patients by TCRγ-PCR, followed by temperature gradient gel electrophoresis (TGGE). In 188 out of 208 MF patients, at least one skin sample with sufficient DNA quality for PCR analysis was obtained. Applying a consensus PCR for the TCRγ genes VγI and Jγ 1/2, we detected monoclonal T cells in 122 cases (65%). In the remaining 66 cases, we performed two multiplex-PCRs, covering rearrangements of the other TCRγ genes. Here we found in 11 cases (6%) predominant clonal rearrangements of VγII–IV and Jγ 1/2 and in 2 (1%) those of VγI–IV and Jγ P1/2. In patients with MF, detecting rearrangements of only VγI and Jγ 1/2 is sufficient for PCR screening analysis. In 53 of the patients (28%) the applied methods revealed no monoclonal T cells. This may be due to a low cell number, oligoclonal nature, chromosomal abberations or remaining of TCR in germline configuration.
    Der Hautarzt 08/1998; 49(8):641-645. · 0.50 Impact Factor
  • Journal of Dermatological Science - J DERMATOLOGICAL SCI. 01/1998; 16.