Yogesh Shouche

Publications (1)1.53 Total impact

• Article: Biodegradation of Carbofuran phenol by free and immobilized cells of Klebsiella pneumoniae ATCC13883T

  Jagannath C. Kadakol, Chandrappa M. Kamanavalli, Yogesh Shouche
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  ABSTRACT: The Klebsiella sp. strain ATCC13883T capable of degrading carbofuran phenol (2,3-dihydro-2,2-dimethylbenzofuran-7-ol) has been separated from the soil by enrichment culture technique and immobilized in various, namely polyurethane foam (PUF), polyacrylamide, alginate, agar and alginate-bentonite clay-powdered activated charcoal (PAC). The degradation rates of 20 and 30mM carbofuran phenol by free and immobilized cells in batch and semi-continuous shaken cultures were compared. The PUF-immobilized cells achieved higher degradation rates in a shorter time than freely suspended cells and the cells immobilized in polyacrylamide, alginate and agar. The PUF- and alginate-bentonite clay-PAC-immobilized cells could be reused for more than 36 cycles, polyacrylamide-entrapped cells for 20 cycles and alginate-bentonite-PAC 28 cycles, without losing any degradation capacity and showed better tolerance to pH, temperature and concentration changes than free cells. These results showed that cells immobilized in modified alginate-bentonite-PAC immobilizers tolerated and completely degraded carbofuran phenol at initial concentrations of 20 and 30mM and also higher. Such a bacterial strain could be used for bioremediation of environments contaminated with phenolic compounds. KeywordsCarbofuran phenol– Klebsiella sp. strain ATCC13883T–Immobilized cells–Polyurethane foam
  World Journal of Microbiology and Biotechnology 04/2012; 27(1):25-29. · 1.53 Impact Factor