Seung Cheol Oh

Publications (1)2.18 Total impact

• Article: Interaction of PRK1 Receptor-like Kinase with a Putative elF2B β-Subunit in Tobacco

  Seong-Whan Park, Sung Hoon Yu, Moon Il Kim, Seung Cheol Oh, Teh-hui Kao, Hyun-sook Pai
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  ABSTRACT: PRK1, a receptor-like kinase that is expressed in pollen, pollen tubes, and ovaries, has been shown to play important roles in pollen development and embryo sac development in Petunia inflata. We have used the kinase domain of PRK1 as a bait in the yeast two-hybrid system to identify PRK1-interacting proteins. The screening resulted in isolation of a cDNA encoding a protein highly homologous to the human and yeast β-subunit of translation initiation factor 2B (eIF2B-β), which was designated NeIF2Bβ. eIF2B is a guanine nucleotide exchange protein that functions in the regulation of translation in eukaryotic cells. Deletion mutants of NeIF2Bβ were analyzed for their interaction with PRK1, and the results suggested that the N-terminal half of NeIF2Bβ, especially the region between residue 103 and 235, is important for the interaction. This protein association was confirmed by in vitro binding assay of the recombinant NeIF2Bβ and PRK1 proteins. Despite high sequence homology between NeIF2Bβ and its yeast counterpart, the NeIF2Bβ cDNA could not rescue the phenotype of the yeast mutant strain lacking the GCD7 gene encoding eIF2B-β, when transferred into the mutant strain. Keywordsβ-Subunit of Eukaryotic Translation Initiation Factor 2B- In vitro Protein Binding Assay-PRK1 Receptor-like Kinase-Yeast Two Hybrid Screening
  Molecules and Cells 04/2012; 10(6):626-632. · 2.18 Impact Factor