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ABSTRACT: The production of recombinant glycoproteins in Dictyostelium discoideum by conventional cell culture methods was limited by low cell density as well as low growth rate. In order to achieve high
cell density cultivation, polyurethane foam (PUF) with high porosity was introduced as new matrix for the immobilization of D. discoideum. The results showed that about 88–93% cells of D. discoideum were adsorbed onto the PUF particles after 100min equilibrium between adsorbed and free cells, and the highest immobilization
rate was achieved by adding the same quantity of PUF matrix with the thin cylinder style. Furthermore, polyurethane foam was
used as the immobilization matrix in a rotating PUF-bed bioreactor system. With batch cultures in the rotating bed bioreactor,
the concentration of immobilized cells in the PUF carrier increased to 4.2×107cellsml−1 after 167h cultivation, which was about fourfold higher than the maximal cell density in the conventional free-cell culture.
Further studies showed that the cells of D. discoideum were not just simply adsorbed on the surfaces, but actively attached to the surfaces through their network of pseudopodia
or filopodia. The present work is very promising to improve the productivity of recombinant proteins in D. discoideum with high cell density in this novel rotating bed bioreactor.
-Immobilization-Polyurethane foam-PUF-bed bioreactor-High density cultivation
World Journal of Microbiology and Biotechnology 01/2010; 26(6):1117-1123. · 1.35 Impact Factor