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ABSTRACT: This article describes a procedure for the quantitation of the isoprostane 15-F2t-IsoP (9a,11a,15S-trihydroxy-(8b)-prosta-5Z,13E-dien-1-oic acid [CAS#27415-26-5] formerly known as 8-epi-PGF2a or 8-iso-PGF2a, and also as iPF2a-III). We have combined features from several earlier methods for 15-F2t-IsoP and prostaglandins, and identified and modified those steps that may lead to poor recoveries. The resulting protocol
is precise and reliable, and was validated by a blind time-course study of plasma levels in rats treated with 120 and 1200
mg CCl4/kg body weight.
Plasma levels of 15-F2t-IsoP, as measured according to the procedure described above, are good indicators of acute oxidative stress as induced by
CCl4. The precision of the measurements allows detection of elevated plasma 15-F2t-IsoP levels as long as 16 h after an acute exposure of 120 mg CCl4/kg body weight, and 2 h after an exposure of 1 mg CCl4/kg body weight.
The results of this low-dose, pilot study suggest that this method has sufficient analytical precision to allow the detection
of the small changes in plasma isoprostane levels, which result from chronic and/or lower-level exposures to agents causing
Molecular Biotechnology 05/2001; 18(2):105-118. · 2.26 Impact Factor