Yang He

Publications (1)1.53 Total impact

• Article: Simultaneous and rapid detection of enteric pathogens from raw milk by multiplex PCR

  Yang He, Juan Dai, Yu-Feng Li
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  ABSTRACT: Enteroinvasive Escherichia coli (EIEC), heat-labile enterotoxin (LT) E. coli, Shigella spp., and Salmonella spp. are common enteric pathogens, which cause food-borne diseases if consumed in contaminated milk products. The rapid and reliable methods for detecting are imperative for reduction in hazard of infection. In this study, we selected primers, optimized the polymerase chain reaction (PCR) conditions, and analyzed the sensitivity and specificity of the multiplex PCR assay to screen raw milk from these enteric bacteria. Furthermore, EIEC, LT-E. coli, Shigella spp., Salmonella spp., and 11 non-targeted pathogenic strains were performed for the specificity of the multiplex PCR. Specific bands showed in EIEC, LT-E. coli, Shigella spp., and Salmonella spp. but no bands showed in other 11 pathogenic strains. The sensitivity of multiplex PCR was relatively high, was rounded to 200CFU/ml (Shigella spp. and EIEC), 320CFU/ml (Salmonella spp.), and 100CFU/ml (LT-E. coli). This method for simultaneous and rapid detection of enteric pathogens (EIEC, LT-E. coli, Shigella spp., and Salmonella spp.) in raw milk showed high sensitivity and specificity, and led to faster track to report results. KeywordsMultiplex PCR–Enteric pathogens–EIEC–LT-E. coli – Salmonella spp.– Shigella spp.–Raw milk
  World Journal of Microbiology and Biotechnology 04/2012; 27(11):2597-2602. · 1.53 Impact Factor