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Publications (10)21.2 Total impact

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    I M Sebetan, H A Hajar
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    ABSTRACT: Polymerase chain reaction (PCR), amplified DNA fragments and denaturing polyacrylamide gel electrophoresis were used to investigate the STR locus HumVWA in a Qatari population sample (n = 200). Seven alleles were identified with frequencies ranging from 0.0125 to 0.3175. The distribution of genotypes fits the Hardy-Weinberg equilibrium. The HumVWA system shows a power of discrimination of 0.9290 and a chance of paternity exclusion of 60.1% in the population studied.
    Forensic Science International 08/1998; 95(2):169-71. · 2.31 Impact Factor
  • Forensic Science International 07/1998; 95(2). · 2.31 Impact Factor
  • I M Sebetan, H A Hajar, E Isobe
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    ABSTRACT: The genotype and allele frequencies at the D1S80 locus were determined in a Qatari population sample (n = 300) using the polymerase chain reaction with subsequent electrophoretic separation of the amplified DNA fragments. Twenty-two different alleles containing 15-39 repeats of the basic 16-bp unit and 78 genotypes were distinguished; the alleles with 18 and 24 repeat units were the most common, with frequencies of 0.2117 and 0.4233, respectively. The observed and expected genotype values show no significant deviation from Hardy-Weinberg equilibrium. The power of discrimination for the D1S80 locus in the Qatari population is 0.9137, and the chance of exclusion is 59.01%.
    Human Biology 03/1998; 70(1):129-35. · 1.52 Impact Factor
  • I M Sebetan, H A Hajar
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    ABSTRACT: Genotype and allele frequencies of HLA DQ alpha locus were determined in a Qatari population sample using polymerase chain reaction (PCR) amplified DNA fragment and hybridization to allele specific oligonucleoid probes in a reversed dot blot format. Among the 200 individuals studied 21 genotypes representing products of 6 HLA DQ alpha alleles have been differentiated, and the frequencies ranged from 0.0775 to 0.2825. The observed and expected genotypes values provide a complete fit to the Hardy-Weinberg equilibrium. The power of discrimination is 0.9321 and chance of paternity exclusion is 60.9% in the Qatari population.
    Forensic Science International 12/1997; 90(1-2):11-5. · 2.31 Impact Factor
  • I M Sebetan, S Oshida, I Yuasa, J Tie
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    ABSTRACT: Isoelectric focusing was used to investigate the genetic variants of the human plasma orosomucoid ORM1 and ORM2 gene loci in samples of Egyptians, Sudanese, and Qataris. The study populations were classified into 28 ORM phenotypes determined by 10 ORM1 and 9 ORM2 alleles that included 2 new alleles, designated ORM1*B13 and ORM2*H21. Family studies of these new alleles are in accordance with codominant autosomal inheritance. A new interpretation for two previously reported alleles, ORM1*C6 and ORM2*H17, is also presented.
    Human Biology 03/1997; 69(1):121-9. · 1.52 Impact Factor
  • Source
    I M Sebetan, S Oshida, J Tie
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    ABSTRACT: An improved high resolution single method for orosomucoid ORM1 and ORM2 phenotyping using isoelectric focusing (IEF) in wide-scales ultrathin layer polyacrylamide gels of pH range 4.2 to 4.9 is presented. The method is reliable, simple, and provides an alternative for the three currently required ones for typing this genetic system.
    Journal of Forensic Sciences 02/1997; 42(1):115-7. · 1.24 Impact Factor
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    ABSTRACT: DNA typing of the D1S80 locus was carried out using capillary polyacrylamide gel zone electrophoresis (CZE). The D1S80 allelic ladder was isolated within 40 min in polyacrylamide gel columns of 50 μm inner diameter and 30 cm length. The PCR products and ladder marker in the injection port were electrophoresed and detected by UV absorption (254 nm). The typing was determined according to migration of DNA fragments on the electropherograms, which permits simultaneous analysis of both the test sample polymerase chain reaction products and the ladder marker. The two-step sample injection method applied in this study allows us to use minute amounts of the ladder maker. This procedure provides a reliable, economical and rapid method for D1S80 typing.
    Forensic Science International 01/1997; 87(3):193-199. · 2.31 Impact Factor
  • Ismail M Sebetan, Hajar A Hajar
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    ABSTRACT: Genotype and allele frequencies of HLA DQα locus were determined in a Qatari population sample using polymerase chain reaction (PCR) amplified DNA fragment and hybridization to allele specific oligonucleoid probes in a reversed dot blot format. Among the 200 individuals studied 21 genotypes representing products of 6 HLA DQα alleles have been differentiated, and the frequencies ranged from 0.0775 to 0.2825. The observed and expected genotypes values provide a complete fit to the Hardy–Weinberg equilibrium. The power of discrimination is 0.9321 and chance of paternity exclusion is 60.9% in the Qatari population.
    Forensic Science International 01/1997; 90(1):11-15. · 2.31 Impact Factor
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    ABSTRACT: Allele frequency distributions for the D1S80 (MCT118) and HLA DQ loci were determined in a Chinese population sample using the polymerase chain reaction (PCR). A total of 25 alleles and 100 phenotypes were observed for D 1 S80. The frequency of allele 18 was higher than allele 24 only in this Chinese population when compared to other reported populations. A total of 6 alleles and 21 possible phenotypes were observed for HLA DQ. The power of discrimination was 0.97 and 0.93 for D1S80 and HLA DQ, respectively.
    Deutsche Zeitschrift für die Gesamte Gerichtliche Medizin 04/1995; 108(3):170-171. · 2.69 Impact Factor
  • J Tie, S Oshida, S Chiba, S Tsukamoto, I M Sebetan
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    ABSTRACT: Allele frequency distributions for the D1S80 (MCT118) and HLA DQ alpha loci were determined in a Chinese population sample using the polymerase chain reaction (PCR). A total of 25 alleles and 100 phenotypes were observed for D1S80. The frequency of allele 18 was higher than allele 24 only in this Chinese population when compared to other reported populations. A total of 6 alleles and 21 possible phenotypes were observed for HLA DQ alpha. The power of discrimination was 0.97 and 0.93 for D1S80 and HLA DQ alpha, respectively.
    Deutsche Zeitschrift für die Gesamte Gerichtliche Medizin 02/1995; 108(3):170-1. · 2.69 Impact Factor