Publications (19)34.7 Total impact

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    Ismail M Sebetan, Hajar A Hajar
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    ABSTRACT: Polymerase chain reaction (PCR), amplified DNA fragments and denaturing polyacrylamide gel electrophoresis were used to investigate the STR locus HumVWA in a Qatari population sample (n = 200). Seven alleles were identified with frequencies ranging from 0.0125 to 0.3175. The distribution of genotypes fits the Hardy-Weinberg equilibrium. The HumVWA system shows a power of discrimination of 0.9290 and a chance of paternity exclusion of 60.1% in the population studied.
    Forensic Science International 08/1998; 95(2):169-71. DOI:10.1016/S0379-0738(98)00095-4 · 2.12 Impact Factor
  • I M Sebetan, H A Hajar, E Isobe
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    ABSTRACT: The genotype and allele frequencies at the D1S80 locus were determined in a Qatari population sample (n = 300) using the polymerase chain reaction with subsequent electrophoretic separation of the amplified DNA fragments. Twenty-two different alleles containing 15-39 repeats of the basic 16-bp unit and 78 genotypes were distinguished; the alleles with 18 and 24 repeat units were the most common, with frequencies of 0.2117 and 0.4233, respectively. The observed and expected genotype values show no significant deviation from Hardy-Weinberg equilibrium. The power of discrimination for the D1S80 locus in the Qatari population is 0.9137, and the chance of exclusion is 59.01%.
    Human Biology 03/1998; 70(1):129-35. · 1.52 Impact Factor
  • I M Sebetan, H A Hajar
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    ABSTRACT: Genotype and allele frequencies of HLA DQ alpha locus were determined in a Qatari population sample using polymerase chain reaction (PCR) amplified DNA fragment and hybridization to allele specific oligonucleoid probes in a reversed dot blot format. Among the 200 individuals studied 21 genotypes representing products of 6 HLA DQ alpha alleles have been differentiated, and the frequencies ranged from 0.0775 to 0.2825. The observed and expected genotypes values provide a complete fit to the Hardy-Weinberg equilibrium. The power of discrimination is 0.9321 and chance of paternity exclusion is 60.9% in the Qatari population.
    Forensic Science International 12/1997; 90(1-2):11-5. · 2.12 Impact Factor
  • Ismail M Sebetan, Hajar A Hajar
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    ABSTRACT: Genotype and allele frequencies of HLA DQα locus were determined in a Qatari population sample using polymerase chain reaction (PCR) amplified DNA fragment and hybridization to allele specific oligonucleoid probes in a reversed dot blot format. Among the 200 individuals studied 21 genotypes representing products of 6 HLA DQα alleles have been differentiated, and the frequencies ranged from 0.0775 to 0.2825. The observed and expected genotypes values provide a complete fit to the Hardy–Weinberg equilibrium. The power of discrimination is 0.9321 and chance of paternity exclusion is 60.9% in the Qatari population.
    Forensic Science International 11/1997; 90(1):11-15. DOI:10.1016/S0379-0738(97)00132-1 · 2.12 Impact Factor
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    ABSTRACT: DNA typing of the D1S80 locus was carried out using capillary polyacrylamide gel zone electrophoresis (CZE). The D1S80 allelic ladder was isolated within 40 min in polyacrylamide gel columns of 50 μm inner diameter and 30 cm length. The PCR products and ladder marker in the injection port were electrophoresed and detected by UV absorption (254 nm). The typing was determined according to migration of DNA fragments on the electropherograms, which permits simultaneous analysis of both the test sample polymerase chain reaction products and the ladder marker. The two-step sample injection method applied in this study allows us to use minute amounts of the ladder maker. This procedure provides a reliable, economical and rapid method for D1S80 typing.
    Forensic Science International 06/1997; 87(3):193-199. DOI:10.1016/S0379-0738(97)00048-0 · 2.12 Impact Factor
  • I M Sebetan, S Oshida, I Yuasa, J Tie
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    ABSTRACT: Isoelectric focusing was used to investigate the genetic variants of the human plasma orosomucoid ORM1 and ORM2 gene loci in samples of Egyptians, Sudanese, and Qataris. The study populations were classified into 28 ORM phenotypes determined by 10 ORM1 and 9 ORM2 alleles that included 2 new alleles, designated ORM1*B13 and ORM2*H21. Family studies of these new alleles are in accordance with codominant autosomal inheritance. A new interpretation for two previously reported alleles, ORM1*C6 and ORM2*H17, is also presented.
    Human Biology 03/1997; 69(1):121-9. · 1.52 Impact Factor
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    I M Sebetan, S Oshida, J Tie
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    ABSTRACT: An improved high resolution single method for orosomucoid ORM1 and ORM2 phenotyping using isoelectric focusing (IEF) in wide-scales ultrathin layer polyacrylamide gels of pH range 4.2 to 4.9 is presented. The method is reliable, simple, and provides an alternative for the three currently required ones for typing this genetic system.
    Journal of Forensic Sciences 02/1997; 42(1):115-7. · 1.31 Impact Factor
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    ABSTRACT: Allele frequency distributions for the D1S80 (MCT118) and HLA DQ loci were determined in a Chinese population sample using the polymerase chain reaction (PCR). A total of 25 alleles and 100 phenotypes were observed for D 1 S80. The frequency of allele 18 was higher than allele 24 only in this Chinese population when compared to other reported populations. A total of 6 alleles and 21 possible phenotypes were observed for HLA DQ. The power of discrimination was 0.97 and 0.93 for D1S80 and HLA DQ, respectively.
    Deutsche Zeitschrift für die Gesamte Gerichtliche Medizin 04/1995; 108(3):170-171. DOI:10.1007/BF01844833 · 2.60 Impact Factor
  • J Tie, S Oshida, S Chiba, S Tsukamoto, I M Sebetan
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    ABSTRACT: Allele frequency distributions for the D1S80 (MCT118) and HLA DQ alpha loci were determined in a Chinese population sample using the polymerase chain reaction (PCR). A total of 25 alleles and 100 phenotypes were observed for D1S80. The frequency of allele 18 was higher than allele 24 only in this Chinese population when compared to other reported populations. A total of 6 alleles and 21 possible phenotypes were observed for HLA DQ alpha. The power of discrimination was 0.97 and 0.93 for D1S80 and HLA DQ alpha, respectively.
    Deutsche Zeitschrift für die Gesamte Gerichtliche Medizin 02/1995; 108(3):170-1. · 2.60 Impact Factor
  • I M Sebetan, K Sagisaka
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    ABSTRACT: Genetic polymorphisms of orosomucoid ORM1 and ORM2 in a Japanese population from northern Japan were investigated using isoelectric focusing (IEF) in ultrathin layer polyacrylamide gels containing Triton X-100 and immunofixation. Nine ORM1 phenotypes which are determined by four common and one rare alleles were observed. Two of the identified alleles at this locus were considered to be new. The ORM2 pattern was classified into 14 phenotypes as products of one common and two variant alleles. The estimated allele frequencies were ORM1*1 = 0.668, ORM1*2 = 0.170, ORM1*2.1 = 0.136, ORM1*5.2 = 0.022 and ORM1*7 = 0.004; ORM2*1 = 0.972, ORM2*3 = 0.006 and ORM2*6 = 0.022.
    Zeitschrift fur Rechtsmedizin 02/1989; 102(1):5-9. DOI:10.1007/BF00201256
  • I M Sebetan, Y Aoki, M Funayama
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    ABSTRACT: Plasminogen (PLG) phenotyping has been performed on 450 unrelated individuals from northern Japan, using wide-scale ultrathin layer polyacrylamide gel isoelectric focusing combined with immunoblotting. One common phenotype and six rare ones were observed. The rare phenotypes included the recently detected allele PLG*M6 in a new combination with PLG*M5 allele. The estimated allele frequencies for PLG*A, PLG*A3, PLG*M2, PLG*M5, PLG*M6, PLG*B, and PLG*B2 were 0.961, 0.009, 0.001, 0.016, 0.001, 0.003, and 0.009, respectively.
    Zeitschrift fur Rechtsmedizin 02/1989; 103(1):43-5. DOI:10.1007/BF01255845
  • I M Sebetan
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    ABSTRACT: A new method for separating genetic variants of the A subunit of human coagulation factor XIII using ultrathin layer polyacrylamide gel isoelectric focusing in 1 M urea followed by immunoblotting is described. The pattern obtained by this method differs from that reported previously: Three sets of unrelated band patterns are observed and can be explained by the existence of two additional gene loci, designated FXIIIA2 and FXIIIA3, besides the previously reported FXIIIA locus, now renamed FXIIIA1. The FXIIIA2 locus is polymorphic and shows three commonly occurring phenotypes, FXIIIA2 1, FXIIIA2 2-I, and FXIIIA2 2. These are determined by two common alleles, FXIIIA2*1 and FXIIIA2*2, with respective frequencies of 0.7965 and 0.2035 in the Japanese population. The studied population conforms to a Hardy-Weinberg equilibrium, and family data confirmed autosomal codominant transmission. The FXIIIA3 locus is monomorphic.
    Human Genetics 01/1989; 81(1):95-7. · 4.52 Impact Factor
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    Ismail M. Sebetan, Kaoru Sagisaka
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    ABSTRACT: Summary Genetic polymorphisms of human serum orosomucoid ORM1 and ORM2 gene loci were investigated in a Libyan population sample from Tripoli, n=110, using isoelectric focusing in ultrathin layer polyacrylamide gel containing either Triton X-100 orN-(2-acetamido)-2-aminoethanesulfonic acid (ACES) with subsequent immunofixation. In ORM1 locus, six common phenotypes ORM1 1-1, ORM1 2-1, ORM1 2-2, ORM1 2.1-1, ORM1 2.1-2 and ORM1 3-1 were identified, with allele frequencies as follows:ORM1*1=0.6500,ORM1*2=0.3091,ORM1*2.1=0.0318 andORM1*3=0.0091. In ORM2 locus, one common phenotype ORM2 1 and four new heterozygote types represent products of three new variant alleles tentatively designatedORM2* Tripoli 1, ORM2* Tripoli 2 andORM2*Tripoli 3 were observed. In addition, one subject in which no ORM2 was detectable, the phenotype ORM2 0-0 is suggested. The ORM2 allele frequencies wereORM2*1=0.9816,ORM2*Tripoli 1=0.0092,ORM2*Tripoli 2=0.0046 andORM2*Tripoli 3=0.0046.
    The Japanese Journal of Human Genetics 01/1989; 33(4):439-43. DOI:10.1007/BF01897784
  • Ismail M. Sebetan
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    ABSTRACT: A new method for separating genetic variants of the A subunit of human coagulation factor XIII using ultrathin layer polyacrylamide gel isoelectric focusing in 1M urea followed by immunoblotting is described. The pattern obtained by this method differs from that reported previously: Three sets of unrelated band patterns are observed and can be explained by the existence of two additional gene loci, designated FXIIIA2 and FXIIIA3, besides the previously reported FXIIIA locus, now renamed FXIIIA1. The FXIIIA2 locus is polymorphic and shows three commonly occurring phenotypes, FXIIIA2 1, FXIIIA2 2-I, and FXIIIA2 2. These are determined by two common alleles, FXIIIA2*1 and FXIIIA2*2, with respective frequencies of 0.7965 and 0.2035 in the Japanese population. The studied population conforms to a Hardy-Weinberg equilibrium, and family data confirmed autosomal codominant transmission. The FXIIIA3 locus is monomorphic.
    Human Genetics 11/1988; 81(1):95-97. DOI:10.1007/BF00283739 · 4.52 Impact Factor
  • I M Sebetan
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    ABSTRACT: An improved method for the separation of the genetic variants of the human serum alpha 2 HS-glycoprotein using isoelectric focusing in ultrathin-layer polyacrylamide gel containing carrier ampholyte pH 4 to 6.5 gradient and neuraminidase pretreated samples is described. The pattern obtained is simple, easily interpretable, and reproducible.
    Journal of Forensic Sciences 08/1988; 33(4):1031-4. · 1.31 Impact Factor
  • Ismail M. Sebetan, Kaoru Sagisaka
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    ABSTRACT: The polymorphism of the human vitamin D binding protein (Gc system) was investigated in a total of 149 sera from unrelated healthy Egyptians residing in Tanta City, Gharbiya Governorate, Nile Delta of Egypt, using isoelectric focusing (IEF) in thin-layer polyacrylamide gel followed by immunoblotting. The estimated gene frequencies were Gc1s = 0.540, Gc1f = 0.242 and Gc2 = 0.218.
    Forensic Science International 04/1988; 37(1):33-6. DOI:10.1016/0379-0738(88)90105-3 · 2.12 Impact Factor
  • I M Sebetan, M M Heshmat
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    ABSTRACT: The genetically determined polymorphism of alpha 2 HS-glycoprotein was analyzed by immunoblotting ultrathin-layer polyacrylamide gel isoelectric focusing in the pH range 4-6.5 and neuraminidase pretreated sera. In a Libyan population sample from Tripoli (n = 110) three common phenotypes, alpha 2 HSG 1-1, 2-1, and 2-2, were observed. The allele frequencies were alpha 2 HSG1 = 0.8364 and alpha 2 HSG2 = 0.1636. The theoretical exclusion rate in cases of disputed paternity is 11.8%.
    Zeitschrift fur Rechtsmedizin 02/1988; 101(3):205-7. DOI:10.1007/BF00201115
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    ABSTRACT: An improved method for detecting diatom in blood is reported. Blood of cadavers was obtained by cardiac puncture at inquest or from the left atrium directly at autopsy. The blood was hemolyzed by sodium dodecyl sulfate (SDS) and filtered through membrane filter (47 mm in diameter and 5 microns in pore size). When the blood was putrefied, two or three pieces of membranes filters were needed because of choking membrane pore. The membrane filters were digested with fuming nitric acid and diluted with distilled water. The diluted solution was filtered through membrane filter (25 mm in diameter) again. After drying the membrane filter was immersed in oil. Diatoms on the membrane filter were clearly observed microscopically.
    Forensic Science International 08/1987; 34(3):175-82. DOI:10.1016/0379-0738(87)90071-5 · 2.12 Impact Factor
  • Ismail M. Sebetan, Masato Funayama, Kaoru Sagisaka
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    ABSTRACT: The polymorphism of the human red cell phosphoglucomutase 1 (PGM1) in samples from Egyptians (n = 134) was investigated using isoelectric focusing in thin-layer polyacrylamide gel. In the studied population samples nine common phenotypes were observed, and the calculated frequencies for the genes PGM1+1, PGM1-1, PGM2+1 and PGM2-1 were 0.6381, 0.0821, 0.2201 and 0.0597, respectively. The observed and expected phenotypes provide a good fit to Hardy-Weinberg equilibrium. The four alleles system will increase the probability of excluding a man falsely accused of paternity to 30% as compared with 16% if the two alleles system is used.
    Forensic Science International 06/1986; 31(1):55-9. DOI:10.1016/0379-0738(86)90072-1 · 2.12 Impact Factor