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ABSTRACT: PurposeThis case report describes two successful pregnancies after intracytoplasmic sperm injection (ICSI) with testicular spermatozoa
that were transported under refrigeration.
MethodsTwo first-time couples consulted our clinic concerned about their primary infertility. No sperm were present in the semen
samples from either of the husbands and they were referred to the urology department (UD) of a neighbouring hospital. At the
UD, seminiferous tubules were obtained by testicular sperm extraction. The tissue samples were put in a centrifuge tube with
phosphate-buffered saline at 6°C and placed with refrigerant in a cushioned styrofoam box that was then transported to our
clinic. Immediately upon arrival at our clinic, testicular spermatozoa were extracted. On the same day, ovum pickup was performed
and mature oocytes were extracted that were then inseminated by conventional ICSI. Fertilized eggs were cultured for 2days,
and then cleaved embryos were cryopreserved. In one case after 4months and in the other case after 2months of cryopreservation,
the frozen-thawed embryos were transferred.
ResultBoth patients became pregnant and normal, healthy babies were born.
ConclusionsThese results suggest that cases of obstructive azoospermia can be treated with ICSI by refrigerated transport of the seminiferous
tubules, in cooperation with a UD, in a small single departmental obstetrics and gynecology clinic.
KeywordsIntracytoplasmic sperm injection-Obstructive azoospermia-Refrigerated transport-Seminiferous tubules-Testicular sperm extraction
Reproductive Medicine and Biology 01/2010; 9(3):173-177.