Manfred Nimtz

Publications (1)3.94 Total impact

• Article: Profiling ubiquitin linkage specificities of deubiquitinating enzymes with branched ubiquitin isopeptide probes.

  Alexander Iphöfer, Anne Kummer, Manfred Nimtz, Antje Ritter, Tatjana Arnold, Ronald Frank, Joop van den Heuvel, Benedikt M Kessler, Lothar Jänsch, Raimo Franke
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  ABSTRACT: Branching out: Novel branched ubiquitin isopeptide activity-based probes (UIPPs) were engineered to profile ubiquitin (Ub) linkage specificity of deubiquitinating enzymes (DUBs) in cell extracts. Using chemical proteomics, a preference towards K48-linked Ub was shown for USP5, USP7, UCH L-3, and UCH L-5, whereas USP19 and USP38 preferred K63-linked Ub.
  ChemBioChem 06/2012; 13(10):1416-20. · 3.94 Impact Factor