Arlette Tais

University of Freiburg, Freiburg, Baden-Württemberg, Germany

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Publications (4)15.62 Total impact

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    ABSTRACT: Ribosome-stalling is an important incidence enabling the cellular quality control machinery to detect aberrant mRNA. Yeast Hbs1•Dom34 and Ski7 are homologs of the canonical release factor eRF3•eRF1, which recognize stalled ribosomes, promote ribosome-release, and induce the decay of aberrant mRNA. Polyadenylated nonstop mRNA encodes for aberrant proteins containing C-terminal polylysine segments which cause ribosome-stalling due to electrostatic interaction with the ribosomal exit tunnel. Here we describe a novel mechanism, termed premature translation termination, which releases C-terminally truncated translation products from ribosomes stalled on polylysine segments. Premature termination during polylysine synthesis was abolished when ribosome-stalling was prevented due to the absence of the ribosomal protein Asc1. In contrast, premature termination was enhanced, when the general rate of translation elongation was slowed down. The unconventional termination event was independent of Hbs1•Dom34 and Ski7, but was dependent on eRF3. Moreover, premature termination during polylysine synthesis was strongly increased in the absence of the ribosome-bound chaperones RAC and Ssb. Based on the data we suggest a model in which eRF3•eRF1 can catalyze the release of nascent polypeptides even though the ribosomal A-site contains a sense codon when the rate of translation is abnormally slow.
    Molecular and Cellular Biology 08/2014; 34(21). DOI:10.1128/MCB.00799-14 · 5.04 Impact Factor
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    ABSTRACT: Ribosome-bound protein biogenesis factors (RPBs) are essential for the early steps of protein biogenesis. A hallmark of RPBs is their ability to bind to the platform surrounding the exit of the ribosomal polypeptide tunnel. Here we describe the interplay between yeast RPBs, which affect the targeting of proteins to the ER membrane.
    BioSpektrum 09/2012; 18(5). DOI:10.1007/s12268-012-0214-8
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    ABSTRACT: Nascent polypeptide-associated complex (NAC) was initially found to bind to any segment of the nascent chain except signal sequences. In this way, NAC is believed to prevent mistargeting due to binding of signal recognition particle (SRP) to signalless ribosome nascent chain complexes (RNCs). Here we revisit the interplay between NAC and SRP. NAC does not affect SRP function with respect to signalless RNCs; however, NAC does affect SRP function with respect to RNCs targeted to the endoplasmic reticulum (ER). First, early recruitment of SRP to RNCs containing a signal sequence within the ribosomal tunnel is NAC dependent. Second, NAC is able to directly and tightly bind to nascent signal sequences. Third, SRP initially displaces NAC from RNCs; however, when the signal sequence emerges further, trimeric NAC·RNC·SRP complexes form. Fourth, upon docking to the ER membrane NAC remains bound to RNCs, allowing NAC to shield cytosolically exposed nascent chain domains not only before but also during cotranslational translocation. The combined data indicate a functional interplay between NAC and SRP on ER-targeted RNCs, which is based on the ability of the two complexes to bind simultaneously to distinct segments of a single nascent chain.
    Molecular biology of the cell 06/2012; 23(16):3027-40. DOI:10.1091/mbc.E12-02-0112 · 5.98 Impact Factor
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    ABSTRACT: Legionella is a pathogenic Gram-negative bacterium that can multiply inside of eukaryotic cells. It translocates numerous bacterial effector proteins into target cells to transform host phagocytes into a niche for replication. One effector of Legionella pneumophila is the glucosyltransferase Lgt1, which modifies serine 53 in mammalian elongation factor 1A (eEF1A), resulting in inhibition of protein synthesis and cell death. Here, we demonstrate that similar to mammalian cells, Lgt1 was severely toxic when produced in yeast and effectively inhibited in vitro protein synthesis. Saccharomyces cerevisiae strains, which were deleted of endogenous eEF1A but harbored a mutant eEF1A not glucosylated by Lgt1, were resistant toward the bacterial effector. In contrast, deletion of Hbs1, which is also an in vitro substrate of the glucosyltransferase, did not influence the toxic effects of Lgt1. Serial mutagenesis in yeast showed that Phe(54), Tyr(56) and Trp(58), located immediately downstream of serine 53 of eEF1A, are essential for the function of the elongation factor. Replacement of serine 53 by glutamic acid, mimicking phosphorylation, produced a non-functional eEF1A, which failed to support growth of S. cerevisiae. Our data indicate that Lgt1-induced lethal effect in yeast depends solely on eEF1A. The region of eEF1A encompassing serine 53 plays a critical role in functioning of the elongation factor.
    Journal of Biological Chemistry 06/2012; 287(31):26029-37. DOI:10.1074/jbc.M112.372672 · 4.60 Impact Factor