David Edwards,
Stephen Wilcox,
Roberto A Barrero,
Delphine Fleury,
Colin R Cavanagh,
Kerrie L Forrest,
Matthew J Hayden,
Paula Moolhuijzen,
Gabriel Keeble-Gagnère,
Matthew I Bellgard,
Michał T Lorenc,
Catherine A Shang,
Ute Baumann, Jennifer M Taylor,
Matthew K Morell,
Peter Langridge,
Rudi Appels,
Anna Fitzgerald
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ABSTRACT: The large and complex genome of wheat makes genetic and genomic analysis in this important species both expensive and resource intensive. The application of next-generation sequencing technologies is particularly resource intensive, with at least 17 Gbp of sequence data required to obtain minimal (1×) coverage of the genome. A similar volume of data would represent almost 40× coverage of the rice genome. Progress can be made through the establishment of consortia to produce shared genomic resources. Australian wheat genome researchers, working with Bioplatforms Australia, have collaborated in a national initiative to establish a genetic diversity dataset representing Australian wheat germplasm based on whole genome next-generation sequencing data. Here, we describe the establishment and validation of this resource which can provide a model for broader international initiatives for the analysis of large and complex genomes.
Plant Biotechnology Journal 06/2012; 10(6):703-8. · 5.44 Impact Factor
