ABSTRACT: Background: A standard protocol for treating peri-implantitis is not yet established. Methods: 150 titanium discs with smooth or rough surfaces contaminated with microbial biofilm were subcutaneously implanted in rats after undergoing one of the treatments: low-intensity laser (LIL), antimicrobial photodynamic therapy (aPDT), and toluidine blue O (TBO). Sterile and contaminated discs served as negative (NC) and positive (C) controls, respectively. After 7, 28, and 84 days tissue inflammation was microscopically evaluated by measuring the density of collagen fibers (degree of fibrosis) and concentration of PMNs. Results: surface texture did not affect the degree of inflammation, but the area of reactive tissue was significantly greater for rough implants (2.6 ± 3.7 x 10(6) μm(2)) than for smooth ones (1.9 ± 2.6 x 10(6) μm(2)) (p = 0.0377). Group C presented the lowest and NC presented the highest degree of fibrosis with significance only after 7 days. These groups had the highest and lowest scores, respectively, for degree of inflammation. The C group showed the largest area of reactive tissue (9.11 ± 2.10 x 10(6) μm(2)), but it was not significantly larger than the LIL (p = 0.3031) and TBO (p = 0.1333) groups. The aPDT showed the smallest area (4.34 ± 1.49 x 10(6) μm(2)) of reactive tissue among the treatment groups. After 28 days, the groups resembled the NC in all the studied parameters. Conclusions: aPDT behaved better than the other methods after 7 days, but over longer periods all methods produced outcomes equivalent to sterile implants.
Journal of Periodontology 06/2012; · 2.60 Impact Factor