ABSTRACT: Study Design. The immunohistochemical profile of NF-κB/p50, NF-κB/p65, MMP-9, MMP-2 and u-PA proteins was examined in spinal cord tissues coming fromrabbits which underwent chronic cervical spinal cord compression.Objective. To study the potential role of NF-κB and extracellural matrix proteins under the chronic mechanical compression of the cervical spinal cord.Summary of Background Data. Cervical spondylotic myelopathy (CSM) is the most common cause of spinal cord dysfunction among adults over the age of 55. Neuronal loss, myelin destruction, axonal degeneration, and glial scar formation are the principal neuropathological features CSM. However, the biologic pathways which lead to these features remain unclear.Methods. In this study we used a new animal experimental model of Cervical Spondylotic Myelopathy (CSM) developed in our laboratory. Briefly, following posterior cervical laminectomy gradual and progressive compression (over 20 weeks) was achieved by introducing a piece of aromatic polyether (0.07mm thick) under the C6 lamina in 15 New Zealand rabbits. In control animals (n = 15) the aromatic polyether was implanted and then removed after 60 seconds (sham operation). The immunoreactivity of p50 and p65 subunits of NF-kB as well as that of MMP-2, MMP-9, u-PA was evaluated in paraffin embedded spinal cord sections coming from both groups. The evaluation was performed using immunohistochemistry technique and the results were analyzed using SPSS for Windows, release 12.0 (SPSSInc.,Chicago,IL).Results. Increased immunoreactivity of both NF-κB subunits, p50 and p65, as well asMMP-2, MMP-9, u-PA was demonstrated in animals with CSM in comparison with controls. Statistical analysis of the results revealed strong positive correlation between NF-κB subunits immunoreactivity and that of MMP-9, MMP-2 and u-PA..Conclusion. There is a strong correlation between the immunoexpression of NF-κB/p50, NF-κB/p65, MMP-2, MMP-9, u-PA and CSM.
Spine 06/2012; · 2.08 Impact Factor