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ABSTRACT: Process of biofilm formation by different Salmonella strains on abiotic surfaces has been studied. Differences in the structural organization were revealed by the analysis of
the fine cell structure in the planktonic and biofilm cultures. It was shown, by the methods of light and electron microscopy
and also by cytochemistry, that the two strains share similarities in structure and have individual features. Differences
in the density of the extracellular matrix and the sizes of cell aggregates were established. The stages in the processes
of growth and death of biofilms were demonstrated by the investigation of the process dynamics. The signs of aging in biofilms
were disorganization of extracellular matrix and appearance of the planktonic cells. Microscopic and cytochemical methods
used in this work were recommended for the investigation of the effects of various biocide agents on biofilms.
Key wordsbiofilm-light microscopy-cytochemistry-electron microscopy-
Salmonella typhimurium
Applied Biochemistry and Microbiology 04/2012; 46(7):706-711. · 0.56 Impact Factor
