Publications (1)0 Total impact
ABSTRACT: The hepatitis C virus NS3 protein contains an N-terminal serine protease and a C-terminal helicase that unwinds RNA or DNA
duplexes. The HCV NS3 protein is the target for several antiviral drugs in clinical trials, which inhibit the protease function.
A method is reported to simultaneously monitor the helicase and protease function of the NS3 protein in a single reaction
using fluorescence spectroscopy and a single chain recombinant protein where NS3 is fused to its protease activator NS4A.
The method monitors both activities together in real time and is amenable to high-throughput screening. This new procedure
could be used to identify compounds that inhibit both the helicase and protease activity of NS3.
Key wordsHelicase–protease–ATPase–high-throughput screening–antiviral agents
03/2010: pages 223-233;