Frederico Marianetti Soriani

Publications (1)2.81 Total impact

• Source

  Available from: Vicente de Paulo Martins

  Article: Silencing of mitochondrial alternative oxidase gene of Aspergillus fumigatus enhances reactive oxygen species production and killing of the fungus by macrophages

  Taisa Magnani, Frederico Marianetti Soriani, Vicente de Paulo Martins, Anna Carolina de Freitas Policarpo, Carlos Artério Sorgi, Lúcia Helena Faccioli, Carlos Curti, Sérgio Akira Uyemura
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  ABSTRACT: We previously demonstrated that conidia from Aspergillus fumigatus incubated with menadione and paraquat increases activity and expression of cyanide-insensitive alternative oxidase (AOX). Here, we employed the RNA silencing technique in A. fumigatus using the vector pALB1/aoxAf in order to down-regulate the aox gene. Positive transformants for aox gene silencing of A. fumigatus were more susceptible both to an imposed in vitro oxidative stress condition and to macrophages killing, suggesting that AOX is required for the A. fumigatus pathogenicity, mainly for the survival of the fungus conidia during host infection and resistance to reactive oxygen species generated by macrophages.
  Journal of Bioenergetics 04/2012; 40(6):631-636. · 2.81 Impact Factor