Hui Li

Fujian Normal University, Fujiang, Heilongjiang Sheng, China

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Publications (125)91.95 Total impact

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    ABSTRACT: A method combining morphological granulometry with Mie theory to determine optical scattering in biological tissues was proposed. Otsu's method was applied to binarize phase-contrast images. Binary morphological granulometry was used to estimate size density distribution of the tissue samples based on the binary phase-contrast images. Our results showed that the optical parameters associated with light scattering in tissue could be quantitatively determined by combining size density distribution with Mie theory. It was suggested that this unique method could be used to characterize biological tissues for disease diagnosis.
    Journal of X-Ray Science and Technology 01/2015; 23(1):111-8. DOI:10.3233/XST-140474 · 1.46 Impact Factor
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    ABSTRACT: Temperature and vibration robustness of a reflecting all-fiber current sensor (AFCS) using a common single-mode fiber (SMF) are analyzed theoretically and experimentally. The effect of the birefringence condition of the sensing fiber on the temperature and vibration sensitivity of the AFCS is shown via theoretical models. The temperature and vibration sensitivity of the AFCS using an orthoconjugate retroreflector are almost entirely independent of the birefringence condition of the fiber. Thus, this configuration is preferable for a reflecting AFCS using an SMF. The experimentally observed temperature and vibration sensitivity of AFCSs using different fibers and configurations support this conclusion.
    Journal of Lightwave Technology 11/2014; 32(22):3709-3715. DOI:10.1109/JLT.2014.2357687 · 2.86 Impact Factor
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    ABSTRACT: Dermatofibrosarcoma protuberans (DFSP) is a skin cancer usually mistaken as other benign tumors. Abnormal DFSP resection results in tumor recurrence. Quantitative characterization of collagen alteration on the skin tumor is essential for developing a diagnostic technique. In this study, second harmonic generation (SHG) microscopy was performed to obtain images of the human DFSP skin and normal skin. Subsequently, structure and texture analysis methods were applied to determine the differences in skin texture characteristics between the two skin types, and the link between collagen alteration and tumor was established. Results suggest that combining SHG microscopy and texture analysis methods is a feasible and effective method to describe the characteristics of skin tumor like DFSP. SCANNING 9999:XX–XX, 2014. © 2014 Wiley Periodicals, Inc.
    Scanning 11/2014; 37(1). DOI:10.1002/sca.21172 · 1.44 Impact Factor
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    ABSTRACT: Measurement of light distribution in biological tissue contributes to selecting strategy and optimizing dose for biomedical application. In this letter, a photoacoustic method combined with Monte Carlo simulation was used to estimate the three-dimensional light distribution in biological tissue. The light distribution was produced by a cylindrical diffuser which interposed into tissues. The light profiles obtained by the method were compared to those detected by photo diodes. The experimental results demonstrate the feasibility of this method. The approach can play a significant role for photo-dosimetry in biomedical phototherapy.
    Chinese Optics Letters 05/2014; 12(5). DOI:10.3788/COL201412.051702 · 1.07 Impact Factor
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    ABSTRACT: Second-degree scalding is a common dermatological injury. Inappropriate treatment methods in clinical practice always produce scarring, and can lead to skin cancer and other complications in the longer term. In this study optical coherence tomography (OCT) combined with a skin detector was used to monitor the response of second-degree scalded skin tissue irradiated by a CO2 laser. The process of treatment of second-degree scalding was systematically studied from the perspective of tissue optics. The OCT signal intensity was stronger within the whole recovery period in the experimental group undergoing CO2 laser treatment, and the attenuation coefficient (μt) returned to its original value in a shorter time. The results help us to understand tissue injury in a second-degree scald and may help improve the standard treatment.
    Laser Physics 04/2014; 24(5):055603. DOI:10.1088/1054-660X/24/5/055603 · 1.03 Impact Factor
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    ABSTRACT: In this study, we presented a method combining optical coherence tomography with wavelet differentiation method to quantify the elastic properties of tissue. Based on the optimized kernel size for 2D normalized cross-correlation, the wavelet differentiation method was used to estimate the tissue strains. The influences of the dilation parameter of wavelet on calculations of axial strain had been investigated. Experimental results suggest that the dilation parameter of 8 was selected in strain calculation for best quality of axial strain images. The method based on wavelet differentiation shows great potential for optical coherence tomography elastography. In addition, elastic properties images of thyroid with suspected cysts were depicted to distinguish benign lesions qualitatively. Thus, elastic properties imaging based on optical coherence elastography shows great promise for the detailed characterization of lesions and preliminary diagnosis of human thyroid diseases.
    Proceedings of SPIE - The International Society for Optical Engineering 01/2014; DOI:10.1117/12.2038102 · 0.20 Impact Factor
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    ABSTRACT: Action-potential-encoded optical second harmonic generation (SHG) has been recently proposed for use in detecting the axonal damage in patients with demyelinating diseases. In this study, the characterization of signal conduction along axons of two different levels of demyelination was studied via a modified Hodgkin–Huxley model, because some types of demyelinating disease, i.e., primary progressive and secondary progressive multiple sclerosis, are difficult to be distinguished by magnetic resonance imaging (MRI), we focused on the differences in signal conduction between two different demyelinated axons, such as the first-level demyelination and the second-level demyelination. The spatio-temporal distribution of action potentials along demyelinated axons and conduction properties including the refractory period and frequency encoding in these two patterns were investigated. The results showed that demyelination could induce the decrease both in the amplitude of action potentials and the ability of frequency coding. Furthermore, the signal conduction velocity in the second-level demyelination was about 21% slower than that in the first-level demyelination. The refractory period in the second-level demyelination was about 32% longer than the first-level. Thus, detecting the signal conduction in demyelinated axons by action-potential-encoded optical SHG could greatly improve the assessment of demyelinating disorders to classify the patients. This technique also offers a potential fast and noninvasive optical approach for monitoring membrane potential.
    Journal of Innovative Optical Health Sciences 01/2014; 07(01). DOI:10.1142/S1793545813300036 · 0.93 Impact Factor
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    ABSTRACT: In this study, the change of tumors' chemical composition in the temperature range of 20~70°C is quantified for photothermal tumor therapy by photoacoustic spectroscopy (PAS) with the wavelengths of 700~1000 nm. Based on the measured photoacoustic signals, two absorption peaks at the wavelengths of 750nm and 950nm are identified. It is also observed that high temperature (>55°C) is able to induce the physical and chemical degeneration of tumors. According to the in vitro tests, a new chemical species, met-hemoglobin, which is absent in normal blood, is generated at high temperature with enhanced near-infrared absorption.
    Bio-medical materials and engineering 01/2014; 24(6):3411-8. DOI:10.3233/BME-141165 · 0.85 Impact Factor
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    ABSTRACT: Photothermal therapy relies on the principle of converting light energy into heat causing localized lesion destruction. For safe and effective treatment, it is necessary to monitor temperature diffusion in the boundaries of the irradiated region, to minimize damage to surrounding normal tissues. This paper gives a pilot study of the feasibility of photoacoustic imaging for monitoring temperature changes during photothermal therapy. The results showed that our system of photoacoustic imaging (PAI) can play the role of biosensor, for the photoacoustics signal amplitude depend on temperature of tissue-mimicking phantoms. Whats more, photoacoustic signal can determinate the boundary of photoabsorder-enhance tissue during therapeutic procedure.
    09/2013; 760-762:872-875. DOI:10.4028/
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    ABSTRACT: Nowadays CO2 laser has been regarded as the effective treatment methods of different kinds of cosmetology and dermatologic surgery. The second-degree scald is of frequent occurrence in people, but many methods on treat the second-degree scald are not good enough. Therefore, the process of second degree scald skin irradiated by the CO2 laser was monitored in the study. The second-degree scald of mice models were divided into two groups in order to contrastively observe the effect of CO2 laser on treating scald. Skin detector associating with the histopathologic examination were used to observe the changes of skin texture and pathologic morphology structure. Meanwhile the healing time was recorded about the two groups. The texture of skin surface irradiated CO2 laser was more glossy and distinct after healing, and the healing time was much faster as well. It was good agreement between skin detector images and histopathological architecture. The result suggests the CO2 laser can significantly cure the the second-degree scald.
    09/2013; 760-762:101-104. DOI:10.4028/
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    ABSTRACT: We present the general coherence theory for laser beams passing through a moving diffuser. The temporal coherence of laser beams passing through a moving diffuser depends on two characteristic temporal scales: the laser coherence time and the mean time it takes the diffuser to move past a phase correlation area. In most applications, the former is much shorter than the latter. Our theoretical analysis shows the spatial coherence area of light scattered from a moving diffuser decreases while the coherence time remains unchanged. The conclusion has been confirmed by experiments using a Michelson interferometer and it is not in accordance with the original coherence theory in which both the temporal and spatial coherence of light scattered by a moving diffuser decrease. We also developed a method based on the theory of eigenvalues to calculate the speckle contrast on a screen illuminated by light transmitted through a moving diffuser.
    Optics Express 06/2013; 21(11):13032-9. DOI:10.1364/OE.21.013032 · 3.53 Impact Factor
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    ABSTRACT: Diseased tissue has different optical properties and dynamic behaviors from normal tissue, and this can be exploited for diagnostics and therapeutics. We present a model for simultaneously estimating the optical scattering coefficient and Brownian diffusion coefficient of superficial biological tissue. This theoretical model predicts that the U-quadratic distribution approximates the Lorentzian power spectrum due to the Brownian motion, when the frequency width of integration is much less than the power spectrum linewidth. Furthermore, it shows that the logarithmic intensity of all calculated wavelet frequencies is linearly dependent on the scattering coefficient and the standard deviation of the Doppler frequency shift is linearly dependent on the Brownian diffusion coefficient in the region of the single scattering length. An optical coherence tomography system was used to measure the optical scattering coefficient and the Brownian diffusion coefficient of phantoms. The results of our theoretical model are consistent with the experimental results. Thus, the method has the potential for diagnosis of diseases.
    Laser Physics 05/2013; 23(6):065603. DOI:10.1088/1054-660X/23/6/065603 · 1.03 Impact Factor
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    ABSTRACT: Quantitative characterization of skin collagen on photo-thermal response and its regeneration process is an important but difficult task. In this study, morphology and spectrum characteristics of collagen during photo-thermal response and its light-induced remodeling process were obtained by second-harmonic generation microscope in vivo. The texture feature of collagen orientation index and fractal dimension was extracted by image processing. The aim of this study is to detect the information hidden in skin texture during the process of photo-thermal response and its regeneration. The quantitative relations between injured collagen and texture feature were established for further analysis of the injured characteristics. Our results show that it is feasible to determine the main impacts of phototherapy on the skin. It is important to understand the process of collagen remodeling after photo-thermal injuries from texture feature.
    Lasers in Medical Science 03/2013; 29(1). DOI:10.1007/s10103-013-1296-3 · 2.42 Impact Factor
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    ABSTRACT: We combine morphological granulometry with Mie theory in order to analyze phase contrast images of biomedical tissue for cancer diagnosis. This method correlates microscopic phase distributions of the tissue image and macroscopic optical scattering properties of the tissue. Our results show that the particle size density distribution can be used to quantitatively identify morphological changes of cancerous stomach tissues. Our method can distinguish normal tissue from cancerous tissues, using the significant differences in scattering coefficient, reduced scattering coefficient and phase function. Therefore, this method can provide not only quantitative information for the diagnosis of cancer, but also accurate optical scattering parameters for photothermal therapy for cancer.
    Laser Physics 03/2013; 23(4):045605. DOI:10.1088/1054-660X/23/4/045605 · 1.03 Impact Factor
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    ABSTRACT: In this study, we proposed photoacoustic imaging to monitor the temperature increase during photothermal therapy, which was carried out by utilizing a continuous wave laser and photoabsorber-enhanced black ink with a absorption peak in the near-infrared optical range. A focusing photoacoustic imaging is interfaced with a nanosecond pulsed laser to image tissue-mimicking phantoms before and after laser irradiation. The results demonstrated that changes in the photoacoustic signal could reflect temperature changes in tissue. More importantly, photoacoustic signal could be used to determine the temperature at the boundary of photoabsorber-enhance tissue during photothermal irradiation. Thus, the photoacoustic imaging could potentially become an effective tool to guide photoabsorber-enhanced photothermal therapy.
    Proceedings of SPIE - The International Society for Optical Engineering 02/2013; DOI:10.1117/12.2001756 · 0.20 Impact Factor
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    ABSTRACT: We present a method for the noninvasive measurement of blood glucose levels, which are determined by the ultrasound-modulated optical technique. The method is based on the optical scattering coefficient. A sensitivity analysis of the ultrasound-modulated light signals in a scattering medium is conducted. Glucose concentrations in intralipid and hemoglobin solutions are measured using the modulation depth of ultrasound-modulated scattered light. The effects of incident light intensity and sample temperature on the ultrasound-modulated signals are also estimated. Preliminary experimental results suggest that the proposed method is a promising technique for noninvasive blood glucose measurement.
    Chinese Optics Letters 02/2013; 11(2). · 1.07 Impact Factor
  • Li Li, Wengming Xie, Hui Li
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    ABSTRACT: The thyroid is one of the main endocrine glands of human body, which plays a crucial role in the body's metabolism. Thyroid cancer mortality ranks only second to ovarian cancer in endocrine cancer. Routine diagnostic methods of thyroid diseases in present clinic exist misdiagnosis and missed diagnosis to varying degrees. Those lead to miss the best period of cancer treatment--early. Photoacoustic spectroscopy technology is a new tool, which provides an effective and noninvasive way for biomedical materials research, being highly sensitive and without sample pretreatment. In this paper, we use photoacoustic spectroscopy technology (PAST) to detect the absorption spectrum between normal and malignant thyroid tissues. The result shows that the photoacoustic spectroscopy technology (PAST) could differentiate malignant thyroid tissue from normal thyroid tissue very well. This technique combined with routine diagnostic methods has the potential to increase the diagnostic accuracy in clinical thyroid cancer diagnosis.
    Proceedings of SPIE - The International Society for Optical Engineering 12/2012; DOI:10.1117/12.999903 · 0.20 Impact Factor
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    ABSTRACT: In order to study scattering properties of normal and cancerous tissues from human stomach, we collect images for human gastric specimens by using phase-contrast microscope. The images were processed by the way of mathematics morphology. The equivalent particle size distribution of tissues can be obtained. Combining with Mie scattering theory, the scattering properties of tissues can be calculated. Assume scattering of light in biological tissue can be seen as separate scattering events by different particles, total scattering properties can be equivalent to as scattering sum of particles with different diameters. The results suggest that scattering coefficient of the cancerous tissue is significantly higher than that of normal tissue. The scattering phase function is different especially in the backscattering area. Those are significant clinical benefits to diagnosis cancerous tissue
    Proceedings of SPIE - The International Society for Optical Engineering 12/2012; DOI:10.1117/12.981956 · 0.20 Impact Factor
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    ABSTRACT: The incidence rate of the prostatic hyperplasia is increasing in near decade, early detection is important for preventing the prostatic cancer (PCa). In this study, the images of prostate and cavernous nerves were carried out using intrinsic fluorescence and scattering properties of the tissues without any exogenous dye or contrast agent based on nonlinear optical microscope. The texture feature and optical property of the interfibrillar substance in prostate tissue were extracted and analyzed for charactering the prostate structure. It will be the feature parameter to differentiate the normal, the inflammation or cancer of prostate tissue in clinical with the application of miniature endoscope nonlinear optical microscope in vivo.
    Proceedings of SPIE - The International Society for Optical Engineering 12/2012; DOI:10.1117/12.999526 · 0.20 Impact Factor
  • Youwu He, Hui Li
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    ABSTRACT: The effect of temperature on the optical properties of biological tissue has been studied by using milk as an example. Optical properties of milk were measured by using optical coherence tomography(OCT) technology in the temperature range of 20-35°C, and we get the logarithm of the OCT signal, then the rules of change of optical properties with temperature were analyzed. The results show that obvious increase can be observed with increasing temperature in the increase attenuation coefficient. It is a novel method to study the effect of temperature on optical properties of biological tissue.
    Proceedings of SPIE - The International Society for Optical Engineering 12/2012; DOI:10.1117/12.999794 · 0.20 Impact Factor

Publication Stats

282 Citations
91.95 Total Impact Points


  • 2005–2015
    • Fujian Normal University
      Fujiang, Heilongjiang Sheng, China
  • 2009–2011
    • Zhejiang University
      • Department of Environmental Science
      Hang-hsien, Zhejiang Sheng, China
  • 2010
    • South China Normal University
      • Institute of Laser Life Science
      Shengcheng, Guangdong, China
  • 2008
    • Zhejiang Medical University
      Hang-hsien, Zhejiang Sheng, China
  • 2007
    • Jimei University
      Amoy, Fujian, China
  • 1999–2000
    • Fujian Teachers University
      Min-hou, Fujian, China