ABSTRACT: ObjectiveThe aim of the study was to investigate the effect of Casticin on proliferation inhibition of human cervical cancer HeLa cells
in vitro and to unravel the associated mechanisms.
MethodsHuman cervical HeLa cells were cultured in vitro. The inhibitory effect of Casticin on the viability of human cervical cancer HeLa cells was evaluated by the MTT assay. The
colony formation ability was detected by plate colony formation assay. Distribution of cell cycle was analyzed by flow cytometry.
The protein expression levels were analyzed by Western blot.
ResultsCasticin significantly inhibited the growth of human cervical cancer HeLa cells in a dose- and time-dependent manner, and
the IC50 was 2.82 μg/mL. The colony-forming rate was reduced drastically compared with control group (P < 0.05). The cells were markedly arrested at G2/M phase after the treatment of Casticin for 48 h. Western blot showed that
the expression of p21 protein was up-regulated and protein level of Cyclin B1 was depressed by Casticin in a concentration
ConclusionCasticin could inhibit the cell growth and lead to cell arrest in human cervical cancer HeLa cells, and the down-regulation
of Cyclin B1 protein expression and activation of p21 protein might contribute to Casticin induced cell arrest in human cervical
cancer HeLa cells.
Keywordscervical cancer–Casticin–cyclin B1–p21–proliferation
The Chinese-German Journal of Clinical Oncology 04/2012; 10(1):47-50.