ABSTRACT: Expressed sequence tag (EST) analyses were performed with the aim of identifying enzyme genes in the liver of the Bleeker’s
squid Loligo bleekeri. Of the 768 ESTs identified and sequenced, 669 were grouped into 324 clusters. Of these clusters, 123 comprising 245 ESTs
were found to be homologous to genes reported to date. Among these, 43 clusters were annotated as enzymes according to the
Enzyme Commission (EC) numbering system. Two EC groups, oxidoreductases and hydrolases, possessed a large number of ESTs.
A cluster homologous to the glutathione peroxidase, an enzyme in the oxidoreductase group, contained 16 ESTs, which accounted
for 2.4% of the total ESTs sequenced. There are three serine proteases, three cathepsins, two triacylgricerol lipases, and
two chitinases among the clusters homologous to the enzymes in the hydrolase group. Since the squid liver functions in the
digestive process, these enzymes would be involved in food digestion. Our data provide information on the various types of
enzymes expressed in the squid liver and may provide a useful basis for further characterization of these enzymes.
Fisheries Science 04/2012; 76(1):161-165. · 0.94 Impact Factor