Mi Ran Roh

Korea University, Sŏul, Seoul, South Korea

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Publications (1)1.39 Total impact

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    ABSTRACT: We investigated the effects of puerarin (8-C-glucosyl-7,4′-dihydroxy isoflavone), an isoflavone found in Kudzu roots (Pueraria lobata), and its glycosides (enzymatically synthesised, water-soluble derivatives of puerarin) on melanogenesis in vitro. Puerarin and its glycosides reduced mushroom tyrosinase activity by 88 and 67% at 4.8mM, respectively, in a concentration-dependent manner. The puerarin glycosides were less effective than puerarin at the same concentration but showed a comparable inhibitory effect at a concentration at which puerarin is insoluble in water. In cultured B16 melanocytes, the melanin content was reduced significantly; moreover, tyrosinase activity was inhibited significantly by both puerarin and its glycosides when added at a concentration of 480μM. DNA microarray and RT-PCR analyses showed significant downregulation of the expression of microphthalmia-associated transcription factor (MITF) and its target genes. The protein expression of MITF and tyrosinase was also downregulated significantly by 40 and 50%, respectively. Our findings suggest that puerarin and its glycosides cause hypopigmentation via dual mechanisms: by inhibiting tyrosinase activity directly and by altering the expression of melanogenesis-related genes, such as MITF and tyrosinase. Therefore, puerarin and its glycosides may have potential for the development of functional cosmetics causing hypopigmentation. KeywordsPuerarin-Melanogenesis-Hypopigmentation-Tyrosinase-Melanin-B16 murine melanocytes-Microarray analysis
    European Food Research and Technology 01/2010; 231(1):75-83. · 1.39 Impact Factor

Publication Stats

3 Citations
1.39 Total Impact Points

Institutions

  • 2010
    • Korea University
      • Department of Food Bioscience and Technology
      Sŏul, Seoul, South Korea