Hye Jin Kim

Catholic University of Korea, Sŏul, Seoul, South Korea

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Publications (225)548.37 Total impact

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    ABSTRACT: Objectives: The clinical significance of aquaporin-1 (AQP1), aquaporin-3 (AQP3), and aquaporin-5 (AQP5) expression was analyzed in a large number of patients with colon cancer. Methods: AQP1, AQP3, and AQP5 expression was investigated based on the immunohistochemistry of tissue microarray specimens from 486 colon cancer patients who underwent curative surgery. Scores were given to the staining intensity and percentage of positive cells, and the staining score was defined as the sum of these scores then used to categorize the AQP expression as negative, weakly AQP-positive, or strongly AQP-positive. Results: A total of 298 (61.3%) patients were identified as strongly AQP1-positive (staining score ≥6), while 38 (7.8%) were strongly AQP3-positive and 145 (29.8%) were strongly AQP5-positive. The overexpression of AQP1, AQP3, and AQP5 was significantly correlated with lymph node metastasis in a multivariate logistic analysis (AQP1, p = 0.026; AQP3, p = 0.023; AQP5, p = 0.003). While the multivariate survival analysis, which included age, histology, TNM stage, and CEA level showed that the expression of AQP1, AQP3, and AQP5 had no effect on the overall survival and disease-free survival. Conclusions: The current study found a significant correlation between AQP1, AQP3, and AQP5 expression and lymph node metastasis in patients with surgically resected colon cancer. © 2015 S. Karger AG, Basel.
    Oncology 02/2015; DOI:10.1159/000369073 · 2.61 Impact Factor
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    ABSTRACT: Rapid and efficient identification of the geographical origin of Angelica gigas roots (dang-gui) was performed using DART–TOF–MS (direct analysis in real time–time of flight–mass spectrometry) based metabolomics. As an ambient desorption/ionization technique, DART–TOF–MS can provide soft ionization and rapid analysis of samples with little sample preparation so it has been advantageously applied to high-throughput metabolomics analysis. In order to develop an efficient tool for discriminating, particularly geographical origin of raw herbal medicine, we employed DART–TOF–MS fingerprinting on dang-gui from Korean and Chinese markets. Principal component analysis of DART–TOF–MS fingerprints gave distinctive clustering information among two species of A. gigas and A. sinensis so that we used only A. gigas species for the sequential experiment. Orthogonal projections to latent structures-discriminant analysis of A. gigas samples revealed the separation between samples cultivated in two countries. Major discriminating components were elucidated as decursin/decursinol angelate, unidentified molecular ion of m/z 247 (protonated ions of molecular formula of C14H14O4) and another molecular ion of m/z 432. DART–TOF–MS based chemical fingerprinting with the multivariate analysis of dang-gui was shown to be efficient and accurate way to identify its geographical origin, between Korea and China.
    Metabolomics 02/2015; 11(1). DOI:10.1007/s11306-014-0671-9 · 3.97 Impact Factor
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    ABSTRACT: Opioid-based intravenous patient-controlled analgesia (IV-PCA) is a popular method of postoperative analgesia, but many patients suffer from PCA-related complications. We hypothesized that PCA was not essential in patients undergoing major abdominal surgery by minimal invasive approach. Between February 2013 and August 2013, 297 patients undergoing laparoscopic surgery for colorectal cancer were included in this retrospective comparative study. The PCA group received conventional opioid-based PCA postoperatively, and the non-PCA group received intravenous anti-inflammatory drugs (Tramadol) as necessary. Patients reported their postoperative pain using a subjective visual analogue scale (VAS). The PCA-related adverse effects and frequency of rescue analgesia were evaluated, and the recovery rates were measured. Patients in the PCA group experienced less postoperative pain on days 4 and 5 after surgery than those in the non-PCA group (mean [SD] VAS: day 4, 6.2 [0.3] vs. 7.0 [0.3], P = 0.010; and day 5, 5.1 [0.2] vs. 5.5 [0.2], P = 0.030, respectively). Fewer patients in the non-PCA group required additional parenteral analgesia (41 of 93 patients vs. 53 of 75 patients, respectively), and none in the non-PCA group required rescue PCA postoperatively. The incidence of postoperative nausea and vomiting was significantly higher in the non-PCA group than in the PCA group (P < 0.001). The mean (range) length of hospital stay was shorter in the non-PCA group (7.9 [6-10] days vs. 8.7 [7-16] days, respectively, P = 0.03). Our Results suggest that IV-PCA may not be necessary in selected patients those who underwent minimal invasive surgery for colorectal cancer.
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    ABSTRACT: Tumor metastasis involves circulating and tumor-initiating capacities of metastatic cancer cells. Epithelial-mesenchymal transition is related to self-renewal capacity and circulating tumor cell (CTC) characteristics for tumor metastasis. Although tumor metastasis as a life-threatening complicated process occurs through circulation of tumor cells, mechanistic aspects of self-renewal and circulating capacities have been largely unknown. Hepatic TM4SF5 promotes EMT for malignant growth and migration, so that it was rationalized TM4SF5 as a hepatocellular carcinoma (HCC) biomarker might be important for metastatic potentials throughout metastasis. Here, self-renewal capacity by TM4SF5 was mechanistically explored using hepatocarcinoma cells with or without TM4SF5 expression, and explored whether they became CTCs using mouse liver-orthotopic model systems. We found that TM4SF5-dependent sphere growth correlated with CD24-, ALDH activity, and a physical association between CD44 and TM4SF5. The interaction between TM4SF5 and CD44 was through their extracellular domains with N-glycosylation modifications. The TM4SF5/CD44 interaction activated c-Src/STAT3/Twist1/Bmi1 signaling for spheroid formation, while disturbing the interaction, expression, or activity of any component in this signaling pathway inhibited the spheroid formation. In serial xenografts using 200 ∼ 5000 cells per injection, TM4SF5-positive tumors exhibited subpopulations with locally-increased CD44 expressions, supporting for tumor cell differentiation. TM4SF5-positive, but not TM4SF5- or CD44-knocked-down, cells were identified circulating in blood 4 to 6 weeks after orthotopic liver-injection using an in vivo laser scanning endomicroscopy. Anti-TM4SF5 reagent blocked their metastasis to distal intestinal organs. Conclusion: Altogether, our results evidence that TM4SF5 promotes self-renewal and CTC properties supported by TM4SF5+/CD44+(TM4SF5-bound)/ALDH+/CD24- markers, during HCC metastasis. This article is protected by copyright. All rights reserved.
    Hepatology 01/2015; DOI:10.1002/hep.27721 · 11.19 Impact Factor
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    ABSTRACT: Angiogenesis, the process of new blood vessel formation, has been a major target for cancer therapy. Antiangiogenic herbal medicines are useful in the treatment of cancer. In this study, we found that a water extract of Cinnamomum cassia (CCWE) was a potent inhibitor of angiogenesis. In cultured human umbilical vein endothelial cells, CCWE suppressed vascular endothelial growth factor (VEGF)-induced proliferation, migration, invasion, tube formation, and intracellular signaling events such as phosphorylation of ERK, p38 and VEGFR2, and activation of matrix metalloproteinase. Furthermore, CCWE inhibited VEGF-induced vessel sprouting of rat aorta ex vivo. These findings might be of particular interest for drug development because VEGF signaling is a potential target for treatment of angiogenesis-associated diseases.
    Bioscience Biotechnology and Biochemistry 01/2015; DOI:10.1080/09168451.2014.993917 · 1.27 Impact Factor
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    ABSTRACT: Nerve-preserving surgery has been provided for patients with rectal cancer; however, sexual dysfunction remains a common complication of rectal cancer surgery. This study explored the efficacy of udenafil to treat erectile dysfunction in male patients who underwent total mesorectal excision (TME) for rectal cancer. We conducted a randomized, double-blind, placebo-controlled clinical trial involving 80 male patients who had decreased International Index of Erectile Function-5 (IIEF-5) scores after TME for rectal cancer. Patients received placebo (50 mg) or udenafil (50 mg) for 12 weeks. The primary outcome variable was the change in IIEF-5 scores. The secondary outcome variables were Sexual Encounter Profile (SEP) questions 2 (Q2) and 3 (Q3), and the Global Assessment Question (GAQ). Baseline IIEF-5 scores, SEP Q2 and Q3 responses, and spontaneous erection rates were consistent in both groups. At the end of treatment, the change in IIEF-5 scores from the baseline was significantly higher in the udenafil group than it was in the placebo group (mean IIEF-5 score, 4.8 ± 4.0 vs 2.0 ± 1.7; P < .05). Responses to SEP Q2, SEP Q3, and GAQ were significantly higher in the udenafil group than they were in the placebo group (SEP Q2, P = .025; SEP Q3, P = .044; GAQ, P < .001). Treatment-related adverse events (n = 4) were all mild in severity. Oral udenafil was deemed safe and effective for the treatment of erectile dysfunction in patients who underwent TME for rectal cancer. Copyright © 2015 Elsevier Inc. All rights reserved.
    Surgery 01/2015; 157(1):64-71. DOI:10.1016/j.surg.2014.07.007 · 3.11 Impact Factor
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    Gyu Jin Heo, Hye Jin Kim, Jeong Im Hong
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    ABSTRACT: In the present study, we aimed to compare the results from nutritional risk screening based on nursing records with those using the Catholic Medical Center Nutritional Risk Screening (CMCNRS) tool. A cross-sectional study was performed involving 91 patients aged ≥ 18 years from an intensive care unit. We collected general characteristics of the patients and nutrition screening was conducted for each patient by using computerized hospital program for the nursing records as well as the CMCNRS conducted by clinical dietitians. The subjects were aged 64.0 ± 17.5 years, and 52 (57.1%) patients had a NPO (nothing by mouth) status. Neurological disease was the most common diagnosis (25.3%). Compared with the CMCNRS results from the clinical dietitians, the results for the nursing records had a sensitivity of 40.5% (95% CI 32.0-40.5) and a specificity of 100.0% (95% CI 92.8-100.0). The agreement was fair between the CMCNRS results obtained by clinical dietitians and the nursing records (k = 0.423). Analysis of the errors from the screening using the nursing records revealed significant differences for all subjective indicators (p < 0.001), compared with the CMCNRS by the clinical dietitians. Thus, after assessing the methods used for nutrition screening and the differences in the search results regarding malnourished status, we noted that the nursing records had a lower sensitivity than the screening by the CMCNRS.
    01/2015; 4(1):56. DOI:10.7762/cnr.2015.4.1.56
  • 12/2014; 20(4):209-220. DOI:10.15616/BSL.2014.20.4.209
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    ABSTRACT: Charcot–Marie–Tooth disease (CMT) is a group of clinically and genetically heterogeneous peripheral neuropathies. We identified two axonal CMT type 2F (CMT2F) families presented with distally predominant weakness in upper and lower extremities with sensory involvement. This study identified a c.404C>T (p.Ser135Phe) mutation in HSPB1 gene as the underlying cause of the both families by applying of whole exome sequencing. The p.Ser135Phe mutation was completely cosegregated with the affected members in the both families, and it was not found in 300 healthy controls. This mutation has been previously reported as the causes of CMT2F or hereditary motor neuropathy 2B (dHMN2B). The mutation was located in the highly conserved alpha-crystallin domain, and several in silico analyses also predicted that the mutation is likely to be pathogenic. HSPB1 encodes heat shock protein 27 (HSP27) which belongs to the superfamily of small stress induced proteins. These results suggest that the HSPB1 mutation is underlying cause of CMT2F phenotype shown in the present families. We believe that this study will be useful for the molecular diagnosis of peripheral neuropathies.
    Genes & genomics 12/2014; DOI:10.1007/s13258-014-0259-9 · 0.57 Impact Factor
  • 11/2014; 24(11):1180-1186. DOI:10.5352/JLS.2014.24.11.1180
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    ABSTRACT: The objective of current study is to assess the relationship between characteristics of patients with acute ischemic stroke and clinical recurrences to identify predictors for the prognosis by modeling and simulation. Primary endpoint was clinical recurrence of ischemic stroke, and secondary endpoint was occurrence of any of the following clinical recurrence of ischemic stroke, transient ischemic attack, acute coronary syndrome, or vascular deaths. Time to event models were developed by NONMEM® using prospectively collected clinical data from 270 patients over 5 years, where 7.0% and 9.3% of them experienced lesion recurrence on MRI at 1 month (LR1M) and clinical recurrence, respectively. Exponential models best described the data. LR1M and diabetes mellitus history were significant predictors for primary endpoint. Times to recurrence for patients with LRIM(+) and diabetes mellitus (+) were predicted to be 0.095 and 0.317 of those for patients with LRIM (−) and diabetes mellitus (−), respectively. LR1M was only predictor for secondary endpoint with predicted time to recurrence in patients with LR1M(+) compared to 0.141 of LR1M(−). Quantitative prediction of clinical recurrence using MRI could improve personalized therapy by identifying patients at risk of recurrence, and could enable efficient clinical trials by stratifying the patients.
    The Journal of Clinical Pharmacology 11/2014; DOI:10.1002/jcph.427 · 2.47 Impact Factor
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    ABSTRACT: Purpose To prospectively compare diagnostic performance of diffusion-weighted ( DW diffusion weighted ) imaging, gadoxetic acid-enhanced magnetic resonance (MR) imaging, both techniques combined (combined MR imaging), and computed tomography (CT) for detecting colorectal hepatic metastases and evaluate incremental value of MR for patients with potentially curable colorectal hepatic metastases detected with CT. Materials and Methods In this institutional review board-approved prospective study, with informed consent, 51 patients (39 men, 12 women; mean age, 62 years) with potentially resectable hepatic metastases detected with CT underwent liver MR, including DW diffusion weighted imaging and gadoxetic acid-enhanced MR. Two independent readers reviewed DW diffusion weighted , gadoxetic acid-enhanced, combined MR, and CT image sets to detect hepatic metastases. The figure-of-merit ( FOM figure of merit ) value representing overall diagnostic performance, sensitivity, and positive predictive value ( PPV positive predictive value ) for each image set were analyzed by using free-response receiver operating characteristic analysis and generalized estimating equations. Results There were 104 hepatic metastases in 47 patients. The pooled FOM figure of merit values, sensitivities, and PPV positive predictive value s of combined MR ( FOM figure of merit value, 0.93; sensitivity, 98%; and PPV positive predictive value , 88%) and gadoxetic acid-enhanced MR ( FOM figure of merit value, 0.92; sensitivity, 95%; and PPV positive predictive value , 90%) were significantly higher than those of CT ( FOM figure of merit value, 0.82; sensitivity, 85%; and PPV positive predictive value , 73%) (P < .006). The pooled FOM figure of merit value and sensitivity of combined MR ( FOM figure of merit value, 0.92; sensitivity, 95%) was also significantly higher than that of DW diffusion weighted imaging ( FOM figure of merit value, 0.82; sensitivity, 79%) for metastases (≤1-cm diameter) (P ≤ .003). DW diffusion weighted imaging showed significantly higher pooled sensitivity (79%) and PPV positive predictive value (60%) than CT (sensitivity, 50%; PPV positive predictive value , 33%) for the metastases (≤1-cm diameter) (P ≤ .004). In 47 patients with hepatic metastases, combined MR depicted more metastases than CT in 10 and 14 patients, respectively, according to both readers. Conclusion Gadoxetic acid-enhanced MR and combined MR are more accurate than CT in detecting colorectal hepatic metastases, have an incremental value when added to CT alone for detecting additional metastases, and can be routinely performed in patients with potentially curable hepatic metastases detected with CT. © RSNA, 2014.
    Radiology 10/2014; DOI:10.1148/radiol.14140390 · 6.21 Impact Factor
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    ABSTRACT: BACKGROUND: Little data are available about the learning curve for robotic rectal resection. OBJECTIVE: The purpose of this work was to provide a multidimensional analysis of the learning process in patients undergoing robotic total mesorectal excision for rectal cancer. DESIGN: This was a retrospective review of a prospectively collected database designed to evaluate the results of robotic rectal resection. SETTINGS: The study was conducted at a tertiary-care hospital. PATIENTS: From December 2007 to August 2012, 167 patients who underwent robotic total mesorectal excision for rectal cancer were included. MAIN OUTCOME MEASURES: A single hybrid variable including operative time, conversion, perioperative morbidity, and circumferential margin was generated to measure the success of the procedure. A moving average method for operative time and a risk-adjusted cumulative sum analysis were used to derive the learning curve. RESULTS: Overall conversion was noted in 2 cases (1.2%). The cumulative sum plot of a single hybrid variable representing the success of each operation demonstrated that the composite event was more frequent at the beginning of the series and began to decrease after 32 cases. The moving average for robotic console time decreased steadily and showed 2 plateaus; the first plateau was noted after 33 cases, and the second plateau was noted after 72 cases. The learning process was divided into 3 phases based on 2 cutoff points. The robotic console time decreased significantly with each phase (p < 0.001). Complicated rectal cancer was more frequent in the later phases; however, the incidence of postoperative complications remained constant throughout the series (p = 0.82). LIMITATIONS: This study is limited by a single surgeon's experience. CONCLUSIONS: The learning process for robotic total mesorectal excision has a greater effect on the first 32 cases. These results help form a basis for performance monitoring of robotic total mesorectal excision.
    Diseases of the Colon & Rectum 09/2014; 57(9):1066-1074. DOI:10.1097/DCR.0000000000000174 · 3.20 Impact Factor
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    ABSTRACT: Thrombocytosis is considered an adverse prognostic factor in various malignancies. However, the clinical significance of thrombocytosis in rectal cancer patients is unknown. We investigated the predictive value of thrombocytosis for pathologic tumor response to preoperative chemoradiotherapy (CRT) and oncologic outcomes in patients with rectal cancer.
    Annals of Surgical Oncology 08/2014; 22(2). DOI:10.1245/s10434-014-3988-8 · 3.94 Impact Factor
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    Hye Jin Kim, Se Ri Park, Young Pyo Jang
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    ABSTRACT: Direct analysis in real time (DART) TOF/MS has been used for mass information of various non-polar phytochemicals in raw material with no sample preparation. However, low ionisation efficiency for polar compounds including glycosides limits its extensive use in the field of phytochemical analysis. In order to develop a direct analysis method for polar glycosides using in situ derivatisation, which improves ionisation efficiency of hydrophilic glycosides. Anemarrhena Rhizoma was used as a model plant targeting on Timosaponin AIII utilising a Dip-It module. Permethylation was applied to the powdered raw material with tetramethylammonium hydroxide in front of a DART ion source. Also, DART TOF/MS combined with permethylation was applied to timosaponin AIII standard solution to obtain the limit of detection (LOD). In situ methylation of timosaponin AIII and Anemarrhena Rhizoma raw material were successfully used to ionise the glycoside. The LOD was found to be in the range of 2.4-4.8 ng for permethylated timosaponin AIII and this level is four times higher than the range of the underivatisation analysis. Direct analysis of permethylated timosaponin from Anemarrhena Rhizoma was also successfully performed. A simple and quick derivatisation method with tetramethylammonium hydroxide was developed for the direct identification of a hydrophilic saponin from the plant tissue. Better ionisation efficiency conferred by in situ permethylation enabled ionisation of whole molecules of timosaponin AIII from the plant tissue. This simple analytical method will provide a solution to reduce tedious sample preparation steps, not only for non-polar but also hydrophilic natural products directly from the tissue. Copyright © 2013 John Wiley & Sons, Ltd.
    Phytochemical Analysis 07/2014; 25(4). DOI:10.1002/pca.2488 · 2.48 Impact Factor
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    ABSTRACT: Vancomycin-resistant enterococci (VRE) are one of the leading causes of nosocomial infection at intensive care unit (ICU). Rapid and sensitive detection of VRE infection is in high demand for timely and suitable antibiotic treatment. Here, we optimized a distinct DNA-based diagnostic technique, loop-mediated isothermal amplification (LAMP) for rapid detection of the presence of vanA gene, a critical component of the gene cluster required for vancomycin resistance. Amplification efficiency was optimal at 62 ˚C and with 2mM MgSO4. The detection limit of the DNA template was 80pg and LAMP amplicons were detected within 40min; thereby suggesting a potential applicability of LAMP as a sensitive and urgent diagnostic method. Furthermore, positive LAMP reaction was directly detected with the naked-eye by monitoring the formation of a white precipitate or the color change induced by hydroxyl naphtol blue (HNB) dye. Finally, 56 clinical isolates were successfully tested for the presence of vanA gene by LAMP, which was determined to be more sensitive than PCR. Together, our results clearly demonstrate the usefulness of LAMP for the diagnosis of VRE infection.
    Journal of Microbiological Methods 06/2014; 104. DOI:10.1016/j.mimet.2014.05.021 · 2.10 Impact Factor
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    ABSTRACT: Sarpogrelate HCl (SGL) has been used clinically as an anti-platelet drug for the prevention of thrombus, proliferation of vascular smooth muscle cells and platelet aggregation. This study was to investigate the bioavailability of sustained-release solid dispersion (SR-SD) formulation of SGL to sustain the drug release for up to 24 h. The SR-SD formulations with various drug-to-polymer ratios were prepared by hot-melt coating method. Waxy material carriers such as Compritol 888 ATO and stearyl alcohol were added to SGL and different amounts of HPMC K 15 (HPMC) were mixed. Dissolution profile and bioavailability were compared to SGL powder. Compritol 888 ATO showed the controlling effect of the initial release rate of drug from the formulation and the controlling effect was increased for 24 h by addition of HPMC. As the amount of HPMC increased, the drug release rate from SR-SD decreased because HPMC formed gel layer in aqueous media. Pharmacokinetic study showed that the AUC and Tmax of SGL in SR-SD formulation increased as compared to the SGL powder. These data suggest that the SR-SD formulation effectively controls the drug release rate for 24 h, hoping to be useful for the development of once-a-day formulation of SGL.
    Archives of Pharmacal Research 06/2014; 38(1). DOI:10.1007/s12272-014-0415-4 · 1.75 Impact Factor
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    ABSTRACT: ObjectiveIn search of an ideal method of assisted hatching (AH), we compared the effects of conventional micropipette-AH and laser-AH on the blastocyst formation rate (BFR) and blastocyst cell numbers.MethodsFour- to five-week-old ICR female mice were paired with male mice after superovulation using Pregnant mare's serum gonadotropin (PMSG) and hCG. The two-cell embryos were flushed from the oviducts of female mice. The retrieved two-cell embryos underwent one of five AH procedures: single mechanical assisted hatching (sMAH); cross mechanical assisted hatching (cMAH); single laser assisted hatching (sLAH); quarter laser assisted hatching (qLAH); and quarter laser zona thinning assisted hatching (qLZT-AH). After 72 hours incubation, double immunofluorescence staining was performed.ResultsFollowing a 72 hours incubation, a higher hatching BFR was observed in the control, sMAH, cMAH, and sLAH groups, compared to those in the qLAH and qLZT-AH groups (p<0.05). The hatched BFR was significantly higher in the qLAH and qLZT-AH groups than in the others (p<0.05 for each group). The inner cell mass (ICM) was higher in the control and sMAH group (p<0.05). The trophectoderm cell number was higher in the cMAH and qLAH groups (p<0.05).ConclusionOur results showed that the hatched BFR was higher in groups exposed the the qLAH and qLZT-AH methods compared to groups exposed to other AH methods. In the qLAH group, although the total cell number was significantly higher than in controls, the ICM ratio was significantly lower in than controls.
    06/2014; 41(2):68-74. DOI:10.5653/cerm.2014.41.2.68
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    ABSTRACT: Assuming an association between cancer and metabolism, oncogene-directed metabolic reprogramming in cancer has revealed new target strategies. For example, the LKB1-AMPK-mTOR signaling pathway genes are already known to alter the cell metabolism and to play a critical role in the malignant behavior of cancer. Accordingly, based on the assumption that genetic variations in the LKB1-AMPK-mTOR signaling pathway can change the intracellular signal in terms of metabolic reprogramming, the present study analyzed 18 single nucleotide polymorphisms (SNPs) of the STK11, PRKAA1, TSC1/2, and mTOR genes and their impact on the survival of patients with colorectal cancer. Seven hundred seventy-two patients with surgically resected colorectal adenocarcinoma were enrolled in the present study. Eighteen SNPs were selected from an in silico analysis based on previous evidence of association. The SNP genotyping was performed using a SEQUENOM MassARRAY. Among the 18 polymorphisms, three SNPs (STK11 rs741765, PRKAA1 rs461404, and TSC1 rs13295634) were significantly associated with disease-free survival (DFS) or overall survival (OS). In a multivariate analysis, the GG genotype of STK11, TT genotype of PRKAA1, and TG or GG genotype of TSC1 were identified as independent prognostic factors for a worse DFS (hazard ratio = 1.398, 1.408, and 1.388; p = 0.030, 0.013, and 0.002, respectively) and OS (hazard ratio = 1.431, 1.680, and 1.394; p = 0.038, 0.001, and 0.009, respectively). The present results suggest that genetic variants of the STK11, PRKAA1, and TSC1 genes could be used as prognostic biomarkers for patients with surgically resected colorectal cancer.
    Annals of Surgical Oncology 04/2014; DOI:10.1245/s10434-014-3729-z · 3.94 Impact Factor
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    ABSTRACT: The plant immune system needs to be tightly controlled both positively and negatively to maintain normal plant growth and health. We previously identified SUPPRESSOR OF rps4-RLD1 (SRFR1) as a negative regulator specifically of effector-triggered immunity. SRFR1 is localized in both a cytoplasmic microsomal compartment and in the nucleus. Its TPR domain has sequence similarity to TPR domains of transcriptional repressors in other organisms, suggesting that SRFR1 may negatively regulate effector-triggered immunity via transcriptional control. We show here that excluding SRFR1 from the nucleus prevented complementation of the srfr1 phenotype. To identify transcription factors that may interact with SRFR1, we screened by yeast two-hybrid assay an Arabidopsis transcription factor prey library and isolated six class I members of the TEOSINTE BRANCHED1/CYCLOIDEA/PCF (TCP) transcription factor family. Specific interactions were verified in planta. While single or double T-DNA mutant tcp8, tcp14 or tcp15 lines were not more susceptible to bacteria expressing AvrRps4, the triple tcp8 tcp14 tcp15 mutant displayed decreased effector-triggered immunity mediated by the resistance genes RPS4, RPS6, RPM1 and RPS2. In addition, expression of PATHOGENESIS-RELATED PROTEIN2 was attenuated in srfr1-4 tcp8-1 tcp14-5 tcp15-3 plants compared to srfr1-4. TCP transcription factors to date have been implicated largely in developmental processes. Our data indicate that one function of a subset of TCP proteins is to regulate defense gene expression in antagonism to SRFR1, and suggest a mechanism for an intimate connection between plant development and immunity. This article is protected by copyright. All rights reserved.
    The Plant Journal 04/2014; DOI:10.1111/tpj.12527 · 6.82 Impact Factor

Publication Stats

2k Citations
548.37 Total Impact Points


  • 2011–2015
    • Catholic University of Korea
      • • Department of Food & Nutrition
      • • College of Medicine
      Sŏul, Seoul, South Korea
    • National Cancer Center Korea
      • Division of Cancer Biology
      Kōyō, Gyeonggi Province, South Korea
  • 2010–2015
    • Kyungpook National University Hospital
      • Department of Hemato-Oncology
      Sŏul, Seoul, South Korea
  • 2009–2015
    • Kyung Hee University
      • College of Pharmacy
      Sŏul, Seoul, South Korea
  • 2007–2015
    • Yonsei University
      • • Division of Biological Science and Technology
      • • Department of Biochemistry
      • • Department of Biotechnology
      Sŏul, Seoul, South Korea
    • International Vaccine Institute
      Sŏul, Seoul, South Korea
  • 2004–2015
    • Seoul National University
      • • Department of Pharmacy
      • • Dental Research Institute
      • • College of Veterinary Medicine
      Sŏul, Seoul, South Korea
  • 2003–2015
    • Kyungpook National University
      • • School of Medicine
      • • Department of Microbiology
      Daikyū, Daegu, South Korea
    • Dong-A University
      • Department of Cardiology
      Tsau-liang-hai, Busan, South Korea
  • 2014
    • Kongju National University
      Gongju, Chungcheongnam-do, South Korea
    • Chungnam National University Hospital
      Sŏul, Seoul, South Korea
  • 2011–2014
    • Sookmyung Women's University
      • College of Pharmacy
      Sŏul, Seoul, South Korea
  • 2007–2014
    • Asan Medical Center
      • Department of Radiology
      Sŏul, Seoul, South Korea
  • 2004–2014
    • Ulsan University Hospital
      Urusan, Ulsan, South Korea
  • 2003–2014
    • Sungkyunkwan University
      • • Department of Pediatrics
      • • Institute of Basic Science
      Sŏul, Seoul, South Korea
  • 2013
    • Korea Basic Science Institute KBSI
      • Busan Center
      Sŏul, Seoul, South Korea
    • Samsung Medical Center
      • Department of Pediatrics
      Sŏul, Seoul, South Korea
  • 2011–2013
    • Gyeongsang National University
      • • Division of Applied Life Science
      • • Institute of Agriculture and Life Science
      Shinshū, Gyeongsangnam-do, South Korea
  • 2012
    • Hankuk University of Foreign Studies
      • Department of Environmental Science
      Sŏul, Seoul, South Korea
    • Korea University of Science and Technology
      Sŏul, Seoul, South Korea
  • 2008–2011
    • Seoul National University Hospital
      • Department of Internal Medicine
      Sŏul, Seoul, South Korea
    • CHA University
      • College of Medicine
      Sŏul, Seoul, South Korea
  • 2004–2011
    • Chungnam National University
      • • Department of Microbiology
      • • School of Bioscience and Biotechnology
      • • College of Pharmacy
      Sŏngnam, Gyeonggi Province, South Korea
  • 2009–2010
    • University of North Carolina at Charlotte
      • Department of Mechanical Engineering and Engineering Science
      Charlotte, North Carolina, United States
  • 2005–2009
    • Ewha Womans University
      • • College of Health Sciences
      • • College of Pharmacy
      Sŏul, Seoul, South Korea
    • Seoul Veterans Hospital
      Sŏul, Seoul, South Korea
    • Dong-A College
      Tsau-liang-hai, Busan, South Korea
  • 2007–2008
    • University of Ulsan
      • College of Medicine
      Urusan, Ulsan, South Korea
  • 2006
    • Korea Advanced Institute of Science and Technology
      • Department of Biological Sciences
      Sŏul, Seoul, South Korea
  • 1999–2002
    • MEDIPOST Biomedical Research Institute
      Sŏul, Seoul, South Korea
  • 2001
    • Dong-Pusan College
      Tsau-liang-hai, Busan, South Korea
    • Korea Food and Drug Administration
      Seishō-gun, Gyeongsangbuk-do, South Korea