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Forensic Science International Genetics Supplement Series 01/2011;
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Forensic Science International Genetics Supplement Series 01/2011;
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ABSTRACT: Eight X-chromosome STR were investigated in two groups of individuals from two Italian regions, Umbria and Sardinia. These two regions have a very different history. Umbria, situated in the centre of Italy and without defensive natural barriers, in the course of the centuries has been object of many invasions and appropriations by foreign people. On the contrary, Sardinia, an island in the centre of the Mediterranean sea, has endured a minor number of foreign invasions. The ChrX markers were amplified in a pentaplex (DXS6789, DXS7423, DXS6807, DXS101, DXS8377) and a triplex (DXS10074, DXS10075, DXS10079) that represents a haplotype. The DNA was extracted from 200 blood samples using the QIAmp DNA Minikit (Qiagen TM). Amplification products were detected on an ABI PRISM 310 Genetic Analyzer (Applied Biosystems), using the same dye labels, run conditions, standard (GeneScan 500 Liz) and matrix file of AmpFlSTRIdentifiler. Statistical analyses for all the loci and intergroup comparisons between the two regional groups of individuals were performed.
Forensic Science International Genetics Supplement Series 01/2009;
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ABSTRACT: In the course of an investigation to identify a burns-related death victim a particular case of paternity test was introduced. In a locality near Spoleto (Umbria), an oil mill burned down causing the death of four men. One of them was identified comparing his genetic profile with those of his relatives: a daughter and a brother. The wife was not available for the analysis. Data obtained with Identifiler showed a genetic compatibility between the man and his daughter. By chance we observed that a genetic compatibility was also evident between the daughter and the other man analyzed, the uncle. To clarify this situation we implemented the panel of markers analyzed, adding PowerPlex 16, 6 miniSTRs (NC01 and NC02) and 5 STRs of X chromosome.
Forensic Science International Genetics Supplement Series 02/2008;
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ABSTRACT: Nowadays, the use of formalyn fixed tissue for forensic identification is frequently requested. This is why forensic laboratories must often study normal or tumour tissue specimens that are usually archived with this method. The somatic instability of tumour tissue on STR loci and the DNA damages caused by formaldeide are well described. These conditions can cause an incorrect allelic determination that makes a forensic identification fail. In order to evaluate the real incidence of the genetic alterations caused by somatic instability of tumour tissue, and the incidence of the DNA damages caused by formalyn, we studied 25 specimens of patients who have been operated for neoplasia. For each patient, we studied a specimen of fresh tumour tissue and a specimen of formalyn-fixed tumour tissue, and the results of these analyses were compared to a specimen of fresh normal tissue and to a specimen of formalyn-fixed normal tissue of the same patient. D 2005 Published by Elsevier B.V.
International Congress Series 01/2006;
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ABSTRACT: STRs are today one of the most effective tools for individual and populational genetic characterization. This paper presents the results of a population study of 15 STR loci included in AmpFlSTR Identifiler kit (Applied Biosystems) that has the aim of creating a local database. Blood or oral swab samples were obtained from 100 unrelated individuals, who were born in Terni (in the center of Italy) and lived there for at least two generations. DNA was extracted from blood, using QIAamp, miniKit of Qiagen and from oral swab, using Chelex method. For each locus allele frequency was calculated and Hardy–Weinberg equilibrium was evaluated. The Expected Heterozygosity (He), the Observed Heterozygosity (Ho), the Polymorphic Information Content (PIC), the Power of Discrimination (PD) and the Power of exclusion (PE) were also calculated. D 2005 Published by Elsevier B.V.
International Congress Series 01/2006; 1288(1288):340.
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ABSTRACT: ChrX markers represent an efficient supplementation of autosomal and Y-chromosomal STR analysis and ChrX haplotyping can elucidate complicated kinship situations. This is the reason why it needs to increase the population data for ChrX STR allelic frequencies and to create national or local databases. An esaplex PCR was developed to amplify DXS6789, HumARA, DXS7423, DXS6807, DXS101 and DXS8377 in some Italian samples from Perugia and Terni. This system represents a protocol for the ChrX analysis with a shorter procedure. The DNA was extracted from 100 blood samples using the QIAmp DNA Minikit produced by Qiagen. The samples were detected on an ABI PRISM 310 Genetic Analyzer (Applied Biosystems), using the same dye labels, run conditions, standard (GeneScan 500 Liz) and matrix file of AmpFlSTRIdentifiler. We performed statistical analysis for all the loci. D 2005 Published by Elsevier B.V.
International Congress Series 01/2006; 1288(1288):292.
