Christoph Böttcher

Freie Universität Berlin, Berlín, Berlin, Germany

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Publications (151)782.57 Total impact

  • Mingyang Wang · Kai Ludwig · Christoph Böttcher · Michael Veit ·
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    ABSTRACT: The only spike of influenza C virus, the hemagglutinin-esterase-fusion glycoprotein HEF combines receptor binding, receptor hydrolysis and membrane fusion activities. Like other hemagglutinating glycoproteins of influenza viruses HEF is S-acylated, but only with stearic acid at a single cysteine located at the cytosol-facing end of the transmembrane region. Previous studies established the essential role of S-acylation of hemagglutinin (HA) for replication of influenza A and B virus by affecting budding and/or membrane fusion, but the function of acylation of HEF was hitherto not investigated. Using reverse genetics we rescued a virus containing non-stearoylated HEF, which was stable during serial passage and showed no competitive fitness defect, but the growth rate of the mutant virus was reduced by one log. Deacylation of HEF does neither affect the kinetics of its plasma membrane transport nor the protein composition of virus particles. Cryo-electron microscopy showed that the shape of viral particles and the hexagonal array of spikes typical for influenza C virus were not influenced by this mutation indicating that virus budding was not disturbed. However, the extent and kinetics of hemolysis were reduced in mutant virus at 37°C, but not at 33°C, the optimal temperature for virus growth, suggesting that non-acylated HEF has a defect in membrane fusion under suboptimal conditions.
    Cellular Microbiology 10/2015; DOI:10.1111/cmi.12541 · 4.92 Impact Factor
  • Hans von Berlepsch · Christoph Böttcher ·
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    ABSTRACT: The aggregation behavior of an Indocyanine Cy5 dye (2-[5-[1,1-dimethyl-3-(4-sulfobutyl)-1,3-¬dihydro-benzo[e]indol-2-ylidene]-penta-1,3-dienyl]-1,1-dimethyl-3-(4-sulfo¬butyl)-1H¬benzo[e]indolium hydroxide, inner salt, sodium salt) in aqueous solution is investigated using absorption and fluorescence spectroscopies, as well as cryogenic transmission electron microscopy (cryo-TEM). The dye concentration is varied within a broad range from ~ 1 µM to ~ 10 mM. At moderate concentrations typical H-aggregates are formed. After longer storage time the absorption spectra of these solutions change dramatically. The characteristic blue-shifted absorption band at around 600 nm becomes replaced by a three-banded absorption spectrum, which spreads over a wide wavelength range of ~ 600 up to 800 nm. However, at the highest dye concentration and in presence of ~ (10 to 30) mM NaCl the three-banded spectrum is observed directly after preparation. The spectroscopic features can be ascribed to a structural transformation of strongly to weakly coupled H-type aggregates. The transformation is promoted by an increase of the ionic strength. Cryo-TEM data reveal that the weakly coupled H*-aggregates are organized in well-ordered, extended monolayer sheets, whereas the strongly coupled H-aggregates appear to consist of particles of only a few nanometers in size.
    The Journal of Physical Chemistry B 08/2015; 119(35). DOI:10.1021/acs.jpcb.5b05576 · 3.30 Impact Factor
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    ABSTRACT: A supramolecular carbohydrate-functionalized two-dimensional (2D) surface was designed and synthesized by decorating thermally reduced graphene sheets with multivalent sugar ligands. The formation of host-guest inclusions on the carbon surface provides a versatile strategy, not only to increase the intrinsic water solubility of graphene-based materials, but more importantly to let the desired biofunctional binding groups bind to the surface. Combining the vital recognition role of carbohydrates and the unique 2D large flexible surface area of the graphene sheets, the addition of multivalent sugar ligands makes the resulting carbon material an excellent platform for selectively wrapping and agglutinating E. coli. By taking advantage of the responsive property of supramolecular interactions, the captured bacteria can then be partially released by adding a competitive guest. Compared to previously reported scaffolds, the unique thermal IR-absorption properties of graphene derivatives provide a facile method to kill the captured bacteria by IR-laser irradiation of the captured graphene-sugar-E. coli complex.
    Nano Letters 08/2015; 15(9). DOI:10.1021/acs.nanolett.5b02256 · 13.59 Impact Factor
  • Takuya Kimura · Ryuichi Shinohara · Christoph Boettcher · Teruyuki Komatsu ·
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    ABSTRACT: This report describes the synthesis, structure, and O2-binding properties of core–shell clusters composed of human haemoglobin A (HbA) in the centre and human serum albumins (HSAs) at the periphery (HbA-HSAm) as a potential O2-carrier designed as a red blood cell (RBC) substitute. Protein clusters were prepared by covalent linkage between HbA lysins and HSA cysteine-34 using heterobifunctional cross-linker, succinimidyl-4-(N-maleimidomethyl)cyclohexane-1-carboxylate. Major products (m = 2, 3, 4) were isolated using gel filtration chromatography. The low isoelectric points (pI = 5.0–5.2) of the clusters were almost identical to that of HSA, proving the wrapping of HbA by negatively charged HSA. The 3D reconstruction of HbA-HSA3 based on transmission electron microscopy images revealed a complete triangular structure. The proposed geometries enabled us to assign a possible spatial arrangement of the HbA centre and HSA periphery. The HbA-HSAm clusters showed higher O2-affinities (P50 = 8–9 Torr) than the native HbA. Clusters prepared under N2 atmosphere showed low O2-affinity (P50 = 26 Torr) resembling human RBC. Moreover, the cluster containing an αα-cross-linked HbA with a bis(3,5-dibromosalicyl)fumarate showed markedly low O2-affinity (P50 = 35 Torr). These HbA-HSAm clusters with various O2-affinities can support a new generation of RBC substitutes that is better tuned to a role in O2 transport.
    06/2015; 3(30). DOI:10.1039/C5TB00540J
  • Shiv Kumar · Katharina Achazi · Christoph Böttcher · Kai Licha · Rainer Haag · Sunil Kumar Sharma ·
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    ABSTRACT: An amphiphilic molecular transporter has been developed by immobilized Candida antarctica lipase catalyzed copolymerization of PEG diethyl ester and azidotriglycerol via its primary hydroxyl groups. The co-polymer was further grafted by the C12 alkyl chain and polyglycerol dendron moieties via the azide group of the polymer backbone by following ‘Click’ chemistry approach. The secondary hydroxyl groups on the backbone provide an additional site for acylation and fine-tune the physico-chemical properties. The aggregation behavior and transport potential of the resulting amphiphilic polymers were studied by surface tension (pendant drop method), DLS, cryo-TEM, UV and fluorescence measurements. The particle size of the micelles was found to have an average diameter of 8-12 nm. The encapsulation potential of these polymeric systems using Cyanine 3, a highly fluorescent dye, used frequently for cellular imaging, has been studied. The transport capacity was observed to be in the range 7.58 to 19.15 mmol/mol of polymer. The cytotoxic study of the polymeric materials carried by using adenocarcinoma human alveolar basal epithelial cell lines (A549) and doxorubicin as a control drug revealed that a fine balance between hydrophilic (polyglycerol moiety) and the hydrophobic (alkyl chain) forces is critical to maintain minimal cytotoxicity. The cellular uptake of Cy3 encapsulated polymers were also studied by means of confocal laser scanning fluorescence microscopy (CFM) and fluorescence activated cell sorting (FACS) measurements. Among all the polymers, [G2.0] grafted dendronized polymer 5c exhibit prominent transport potential.
    European Polymer Journal 06/2015; 39. DOI:10.1016/j.eurpolymj.2015.06.017 · 3.01 Impact Factor
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    ABSTRACT: Photoresponsive polymeric amphiphiles were developed by first synthesizing the polyester chain via Novozym 435 catalyzed step growth, condensation polymerization of poly[ethylene glycol bis(carboxymethyl) ether]diethylester and 2-azidopropan-1,3-diol followed by its grafting with 4'-butyl-4-propargyloxy(azobenzene) and [G2.0] polyglycerol dendron by using ‘Click chemistry’ approach. The resulting polymers were observed to form supramolecular micellar aggregates in the aqueous solution. The critical aggregation concentration (CAC) was determined via fluorescence measurements and using ‘Nile red’ as a probe. The nano-structures formed in the aqueous solution were characterized by dynamic light scattering (DLS) and cryo-TEM measurements. The encapsulation potential of polymeric amphipihiles for Nile red and curcumin as well as their release via trans-cis photoisomerization of the embedded azobenzene moiety was studied, by absorbance and fluorescence spectroscopy techniques. The developed polymeric micellar systems behave as efficient photoresponsive smart nanocarriers.
    RSC Advances 05/2015; 5(60). DOI:10.1039/C5RA08047A · 3.84 Impact Factor
  • Daniel Schöne · Boris Schade · Christoph Böttcher · Beate Koksch ·
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    ABSTRACT: Strategies to achieve controlled nanoparticle aggregation have gained much interest, due to the versatility of such systems and their applications in materials science and medicine. In this article we demonstrate that coiled-coil peptide-induced aggregation based on electrostatic interactions is highly sensitive to the length of the peptide as well as the number of presented charges. The quaternary structure of the peptide was found to play an important role in aggregation kinetics. Furthermore, we show that the presence of peptide fibers leads to well-defined nanoparticle assembly on the surface of these macrostructures.
    Beilstein Journal of Organic Chemistry 05/2015; 11:792-803. DOI:10.3762/bjoc.11.89 · 2.76 Impact Factor
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    Rainer Haag · Christoph Boettcher · Hans von Berlepsch · Bala N. S. Thota ·
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    ABSTRACT: Engineering nanostructures of defined size and morphology is a great challenge in the field of self-assembly. Herein we report on the formation of supramolecular nanostructures of defined morphologies with subtle structural changes for a new series of dendritic amphiphiles. Subsequently, we studied their application as nanocarriers for guest molecules.
    Chemical Communications 03/2015; 51(41). DOI:10.1039/C4CC09513H · 6.83 Impact Factor
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    ABSTRACT: The first highly water-soluble perylene-calix[4]arene-hybrid with the calixarene scaffold acting as a structure determining central platform is presented. In this tetrahedrally shaped amphiphilic architecture the hydrophilic and hydrophobic subunits are oriented ate the opposite side of the calixarene platform. The hydrophobic part contains the two perylenediimide moieties, which enable strong π-π-interactions in self-assembly processes. Two hydrophilic Newkome-type dendrons provide sufficient water solubility at slightly basic conditions. The tetrahedrally shaped amphiphile dis-plays an unprecedented aggregation behavior down to concentrations as low as 10 7 mol L 1. The intriguing self-assembly process of the compound in water as well as under changed polarity conditions, achieved by addition of THF, could be monitored by the complemented use of cryogenic transmission electron microscopy (cryo-TEM), UV/Vis- and fluores-cence spectroscopy. Molecular-dynamics (MD) and molecular modelling simulations helped in understanding the inter-play of supramolecular and optical behavior.
    Journal of the American Chemical Society 02/2015; 137(9). DOI:10.1021/ja512048t · 12.11 Impact Factor
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    ABSTRACT: Competitive binding inhibitors based on multivalent nanoparticles have shown great potential for preventing virus infections. However, general design principles of highly efficient inhibitors are lacking as the quantitative impact of factors such as virus concentration, inhibitor size, steric shielding or multivalency effects in the inhibition process is not known. Based on two complementary experimental inhibition assays we could determine and even predict size-dependent steric shielding and multivalency effects. This allowed us to adapt the Cheng-Prusoff equation for its application to multivalent systems. Our results showed that the particle and volume normalized IC50 value of an inhibitor at very low virus concentrations predominantly depends on its multivalent association constant, which itself exponentially increases with the inhibitor/virus contact area and ligand density. Compared to multivalency effects, the contribution of steric shielding to the IC50 values is only minor and its impact is only noticeable if the multivalent dissociation constant is far below the virus concentration, which means if all inhibitors are bound to the virus. The dependence of the predominant effect, either steric shielding, or multivalency, on the virus concentration has significant implications on the in vitro testing of competitive binding inhibitors and determines optimal inhibitor diameters for the efficient inhibition of viruses.
    Journal of the American Chemical Society 01/2015; 137(7). DOI:10.1021/ja5114084 · 12.11 Impact Factor
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    Changzhu Wu · Christoph Boettcher · Rainer Haag ·
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    ABSTRACT: The enormous potential of nanogel scaffolds for protein encapsulation has been widely recognized. However, constructing stable polymeric nanoscale networks in a facile, mild, and controllable fashion still remains a technical challenge. Here, we present a novel nanogel formation strategy using horseradish peroxidase (HRP) catalyzed crosslinking on phenolic derivatized dendritic polyglycerol (dPG) in the presence of H2O2 in an inverse miniemulsion. This “enzymatic nanogelation” approach was efficient to produce stable 200 nm dPG nanogel particles, and was performed under physiological conditions, thus making it particularly beneficial for encapsulating biological proteins. Purification of the nanogels was easy to handle and practical because there was no need for a post-quenching step. Interestingly, the use of dPG resulted in higher HRP laden nanogels than for linear polyethylene glycol (PEG) analogs, which illustrates the benefits of dendritic backbones in nanogels for protein encapsulation. In addition, the mild immobilization contributed to the enhanced thermal stability and reusability of HRP. The nanogel preparation could be easily optimized to achieve the best HRP activity. Furthermore, a second enzyme, Candida antarctica lipase B (CalB), was successfully encapsulated and optimized for activity in dPG nanogels by the same enzymatic methodology, which shows the perspective applications of such techniques for encapsulation of diverse proteins.
    Soft Matter 12/2014; 11(5). DOI:10.1039/C4SM01746C · 4.03 Impact Factor
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    ABSTRACT: Two core-shell nanoparticles with polyglycerol shells and sp3 carbon cores with different flexibilities (soft dendritic polyethylene and hard nanodiamond) were synthesized, their encapsulation capacities were compared, and their ability to transport into tumor cells was investigated. The nanocarrier with a soft core was superior to the hard one.
    12/2014; 3(5). DOI:10.1039/C4TB01858C
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    Ana Campo Rodrigo · Shashwat Malhotra · Christoph Boettcher · Mohsen Adeli · Rainer Haag ·
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    ABSTRACT: Here we report for the first time on a microwave assisted synthesis of polyglycerol dendron functionalized cyclodextrins (CD) with hydrophobic tails. These amphiphilic CDs consist of seven polyglycerol dendrons and fourteen alkyl chains on the primary and secondary rims of the cyclodextrin core, respectively. They self-assemble to form nanostructures in aqueous solutions and efficiently encapsulate hydrophobic aromatic guests. Size and shape of self-assemblies and also their ability to encapsulate guest molecules depend on the generation of conjugated polyglycerol dendrons.
    RSC Advances 11/2014; 4(106). DOI:10.1039/C4RA11601A · 3.84 Impact Factor
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    ABSTRACT: Supramolecular polymerization for non-wetting surface coatings is described. The self-assembly of low-molecular-weight gelators (LMWGs) with perfluorinated side chains can be utilized to rapidly construct superhydrophobic as well as liquid-infused slippery surfaces within minutes. The lubricated slippery surface exhibits impressive repellency to biological li-quids, such as human serum and blood, and very fast self-healing.
    Advanced Materials 11/2014; 26(43). DOI:10.1002/adma.201401366 · 17.49 Impact Factor
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    ABSTRACT: To gain insight into the factors that affect stability and transport efficiency under dilution conditions, dendronized and hyperbranched multifunctional amphiphilic polymers are synthesized by following the "grafting to" approach using varied amounts of propargylated alkyl chain with perfect and hyperbranched polyglycerol dendrons on the base copolymer of PEG (M¯n: 1000 g mol(-1) ) diethylester and 2-azidopropane-1,3-diol following the "bio-catalytic method" and "click approach". The dendronized and hyperbranched polymeric systems form supramolecular aggregates and exhibit an efficient transport potential for the model dye "Nile red" in the low μm range in the core-shell-type architecture provided with distinct amphiphilicity as required for encapsulation. Cytotoxicity studies show the polymeric systems to be non-toxic over a wide concentration range. The cellular internalization of Nile-red-encapsulated supramolecular micellar structures is also studied using cellular fluorescence micro-scopy and fluorescence-activated cell sorting (FACS) measurements. A comparison of the data for the dendronized polymers (PEG M¯n: 600/1000 g mol(-1) ) with the respective low-molecular-weight amphiphile reveal that these polymeric systems are excellent nanotransporters.
    Macromolecular Rapid Communications 11/2014; 36(2). DOI:10.1002/marc.201400467 · 4.94 Impact Factor
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    Hitomi Hosaka · Risa Haruki · Kana Yamada · Christoph Böttcher · Teruyuki Komatsu ·
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    ABSTRACT: A covalent core-shell structured protein cluster composed of hemoglobin (Hb) at the center and human serum albumins (HSA) at the periphery, Hb-HSAm, is an artificial O2 carrier that can function as a red blood cell substitute. Here we described the preparation of a novel Hb-HSA3 cluster with antioxidant activities and its O2 complex stable in aqueous H2O2 solution. We used an approach of incorporating a Pt nanoparticle (PtNP) into the exterior HSA unit of the cluster. A citrate reduced PtNP (1.8 nm diameter) was bound tightly within the cleft of free HSA with a binding constant (K) of 1.1×107 M-1, generating a stable HSA-PtNP complex. This platinated protein showed high catalytic activities for dismutations of superoxide radical anions (O2•-) and hydrogen peroxide (H2O2), i.e., superoxide dismutase and catalase activities. Also, Hb-HSA3 captured PtNP into the external albumin unit (K = 1.1×107 M-1), yielding an Hb-HSA3(PtNP) cluster. The association of PtNP caused no alteration of the protein surface net charge and O2 binding affinity. The peripheral HSA-PtNP shell prevents oxidation of the core Hb, which enables the formation of an extremely stable O2 complex, even in H2O2 solution.
    PLoS ONE 10/2014; 9(10):e110541. DOI:10.1371/journal.pone.0110541 · 3.23 Impact Factor
  • Hans v. Berlepsch · Kai Ludwig, · Boris Schade, · Rainer Haag, · Christoph Böttcher ·
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    ABSTRACT: The self-assembly of amphiphilic molecules into fibrous structures has been the subject of numerous studies over past decades due to various current and promising technical applications. Although very different in their head group chemistry many natural as well as synthetic amphiphilic compounds derived from carbohydrates, carbocyanine dyes, or amino acids tend to form fibrous structures by molecular self-assembly in water predominantly twisted ribbons or tubes. Often a transition between these assembly structures is observed, which is a phenomenon already theoretically approached by Wolfgang Helfrich and still focus point in current research. With the development of suitable sample preparation and electron optical imaging techniques, cryogenic transmission electron microscopy (cryo-TEM) in combination with three-dimensional (3D) reconstruction techniques has become a particular popular direct characterization technique for supramolecular assemblies in general. Here we review the recent progress in deriving precise structural information from cryo-TEM data of particularly fibrous structures preferably in three dimensions.
    Advances in Colloid and Interface Science 06/2014; 208. DOI:10.1016/j.cis.2014.01.007 · 7.78 Impact Factor
  • Lennard Wasserthal · Boris Schade · Kai Ludwig · Christoph Böttcher · Andreas Hirsch ·
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    ABSTRACT: A series of novel functionalised dumbbell-shaped bifullerenes in which two [5.0] pentakis-adducts of C60 are covalently connected by cyclic bismalonates were synthesised. These dimeric compounds, carrying various combinations of hydrophilic and hydrophobic addends, self-assemble in aqueous solution towards supramolecular architectures of different structural complexity as observed by cryogenic transmission electron microscopy (cryo-TEM). The detailed analysis of the image data revealed an unprecedented hierarchical aggregation behaviour. Whereas completely hydrophilic substituted bifullerenes formed profoundly monodisperse populations of small oligomeric elementary micelles consisting of only three or four bifullerene molecules in a supposedly bent conformation, their amphiphilic equivalents underwent a hierarchical two-step assembly process towards larger spherical and even rod-like structures. The data suggest that the hierarchical assembly process is driven by hydrophobic interactions of preformed tetrameric elementary micelles.
    Chemistry - A European Journal 05/2014; 20(20):5961-6. DOI:10.1002/chem.201400153 · 5.73 Impact Factor
  • Hans von Berlepsch · Kai Ludwig · Christoph Böttcher ·
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    ABSTRACT: The aggregation behaviour of the cationic pinacyanol chloride in aqueous solution is investigated using absorption and linear dichroism spectroscopies, optical microscopy and cryogenic transmission electron microscopy (cryo-TEM). The investigations are focused on solutions in a concentration range from 50 μM up to 1 mM. At a concentration of 0.7 mM H-aggregates are detected that are characterized by a broad absorption band centred at ∼511 nm. The aggregates possess a tubular architecture with a single-layer wall thickness of ∼2.5 nm and an outer diameter of ∼6.5 nm. Linear dichroism spectroscopy indicates that the molecules are packed with their long axis parallel to the tube axis. These H-aggregates are not stable, but transform into J-aggregates on the time scale of weeks. The kinetics of J-aggregation depends on the dye concentration and the route of sample preparation, but can also be enhanced by shear stress. J-aggregates possess a split absorption spectrum composed of two longitudinally polarized J-bands and one H-band that is polarized perpendicular to the aggregate axis. The J-aggregates are ∼9 nm wide and several micrometer long fibrils consisting of stacked pairs of ribbons with a dumbbell-shaped density cross-section. Upon aging these ribbons laterally stack face-to-face to form tape-like aggregates.
    Physical Chemistry Chemical Physics 04/2014; 16(22). DOI:10.1039/c4cp00967c · 4.49 Impact Factor
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    ABSTRACT: We have synthesized a targeted imaging agent for rheumatoid arthritis based on polysulfated gold nanorods. The CTAB layer on gold nanorods was first replaced with PEG-thiol and then with dendritic polyglycerolsulfate at elevated temperature, which resulted in significantly reduced cytotoxicity compared to polyanionic gold nanorods functionalized by non-covalent approaches. In addition to classical characterization methods, we have established a facile UV-VIS based BaCl2 agglomeration assay to confirm a quantitative removal of unbound ligand. With the help of a competitive surface plasmon resonance-based L-selectin binding assay and a leukocyte adhesion-based flow cell assay, we have demonstrated the high inflammation targeting potential of the synthesized gold nanorods in vitro. In combination with the surface plasmon resonance band of AuNRs at 780 nm, these findings permitted the imaging of inflammation in an in vivo mouse model for rheumatoid arthritis with high contrast using multispectral optoacoustic tomography. The study offers a robust method for otherwise difficult to obtain covalently functionalized polyanionic gold nanorods, which are suitable for biological applications as well as a low-cost, actively targeted, and high contrast imaging agent for the diagnosis of rheumatoid arthritis. This paves the way for further research in other inflammation associated pathologies, in particular, when photothermal therapy can be applied.
    Theranostics 03/2014; 4(6):629-41. DOI:10.7150/thno.8518 · 8.02 Impact Factor

Publication Stats

4k Citations
782.57 Total Impact Points


  • 1990-2015
    • Freie Universität Berlin
      • • Institute of Chemistry and Biochemistry
      • • Division of Organic Chemistry
      • • Division of Inorganic Chemistry
      Berlín, Berlin, Germany
  • 2004-2010
    • Friedrich-Alexander Universität Erlangen-Nürnberg
      • Department of Chemistry and Pharmacy
      Erlangen, Bavaria, Germany
  • 2009
    • Technische Universität Berlin
      • Department of Chemistry
      Berlín, Berlin, Germany
  • 2008
    • Humboldt University of Berlin
      • Faculty of Mathematics and Natural Sciences I
      Berlín, Berlin, Germany
  • 2007
    • Universiteit Utrecht
      • Department of Infectious Diseases and Immunology
      Utrecht, Utrecht, Netherlands
  • 2003-2005
    • University of Leipzig
      • Institute of Organic Chemistry
      Leipzig, Saxony, Germany
  • 2000
    • Max Planck Institute of Colloids and Interfaces
      Potsdam, Brandenburg, Germany
  • 1993
    • Technion - Israel Institute of Technology
      H̱efa, Haifa, Israel
    • Max Planck Society
      München, Bavaria, Germany
  • 1992
    • Fritz Haber Institute of the Max Planck Society
      Berlín, Berlin, Germany