Dragan Manojlovic

University of Belgrade, Beograd, Central Serbia, Serbia

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Publications (75)159.39 Total impact

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    ABSTRACT: We studied the effect of liming and P fertilization of extremely acid soil (accidently acidified by sulfidic mining waste) on P availability and the subsequent adaptive responses of wheat roots. The wheat plants were grown in rhizoboxes allowing precise sampling of rhizosphere and bulk soil for sequential extraction of P fractions and determination of exchangeable Al. Root exudates were collected by pieces of paper for electrophoresis and subjected to HPLC analysis. Expression of organic anions and Pi transporter genes was analyzed by a real-time quantitative PCR. The concomitant application of lime with P fertilization increased the concentrations of plant-available P fractions in both rhizosphere and bulk compartments. The applied soil amendments strongly affected plant growth, biomass partitioning and shoot P accumulation. Liming enhanced root exudation of citrate in P unfertilized plants, while the high malate efflux was maintained until both P deficiency and Al toxicity were eliminated by the amendments. We showed the importance of liming for recovering of P acquisition potential of wheat roots, which can be strongly impaired in acid soils. Our results clearly demonstrated that P-deficient roots not subjected to Al stress in the limed soil can maintain high efflux of malate and even increase efflux of citrate along with the enhanced expression of related anion transporters (TaMATE1 and TaALMT1).
    Biology and Fertility of Soils 03/2015; 51:289–298. DOI:10.1007/s00374-014-0975-y · 3.40 Impact Factor
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    ABSTRACT: The objectives of this study were to determine the concentrations of Pb, Cd, As, Cr, Cu, Co, Ni, Zn, Ba, Fe, Al and Ag in Erigeron canadensis L. growing on fly ash landfill of power plant "Kolubara", Serbia. The content of each element was determined in every part of plant separately (root, stalk and inflorescence) and correlated with the content of elements in each phase of sequential extraction of fly ash. In order to ambiguously select the factors that are able to decidedly characterize the particular part of plant, principal component analysis (PCA) and partial least squares discriminant analysis (PLS-DA) were performed. The bioconcentration factors and translocation factors for each metal were calculated in order to determine the feasibility of the use of plant E. canadensis L. for phytoremediation purpose. There were strong positive correlations between metals in every part of plant samples, and metals from pseudo total form of sequential extraction indicate that the bioavailability of elements in fly ash is similarly correlated with total form. Retained Al, Fe, Cr and Co in the root indicate its suitability for phytostabilization. This plant takes up Cd and Zn from the soil (bioconcentration factors (BCFs) greater than 1), transporting them through the stalk into the inflorescence (translocation factors (TFs) higher than 1). Regarding its dominance in vegetation cover and abundance, E. canadensis L. can be considered adequate for phytoextraction of Cd and Zn from coal ash landfills at Kolubara.
    Environmental Science and Pollution Research 03/2015; DOI:10.1007/s11356-015-4192-5 · 2.76 Impact Factor
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    ABSTRACT: The aim of this paper was the development and optimization of an electrochemical method for the degradation of two triketone herbicides, mesotrione and sulcotrione, in a two-electrode undivided electrochemical cell equipped with commercially available, non-modified, electrode materials. The electrochemical parameters studied included five different electrode systems (Zr/Pt, Pd/Pt, C/Pt, Nb/Pt and Ti/Pt), current densities (27.66, 55.33, …, 110.66 mA·cm-2) and pH values (3, 7 and 9) in 0.05 M sodium sulfate as supporting electrolyte. The electrical energy consumption and chemical oxygen demand were calculated for optimal conditions. The degradation efficiency was determined by high performance liquid chromatography equipped with a diode array detector, while the degradation products for both pesticides were identified and compared by UHPLC-mass spectrometry. The results could lead to an accurate estimate of their effect on the environment. A real water sample was used to study the influence of organic matter on pesticide degradation. Based on the results obtained the electrochemical treatment could be used for the successful removal of triketone herbicides from contaminated water.
    CLEAN - Soil Air Water 12/2014; DOI:10.1002/clen.201300951 · 1.84 Impact Factor
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    ABSTRACT: This paper reports the results of a study focused on the presence and bioavailability of arsenic in agricultural soil in the mining and industrial regions of northern Kosovo and southern Serbia, as well as uptake and bioaccumulation of arsenic in two commonly cultivated plant species (Zea mays L. and Solanum tuberosum L.). This area was one of the most important mining districts in Europe. The collected soil samples were subjected to a modified BCR three-step sequential extraction procedure in order to investigate the chemical partitioning of arsenic in the soils. The general distribution of arsenic in various fractions was: exchangeable < reducible < oxidizable fractions. Highest concentrations of total arsenic in soil were found close to industrial facilities and tailing ponds. In addition, fluvisols were significantly more enriched with arsenic than soils at a distance from the river flows. The edible parts of the plant specimen showed different As contents, suggesting that these plant species have different propensities for the uptake and bioaccumulation of arsenic from soil.
    Soil and Sediment Contamination 12/2014; DOI:10.1080/15320383.2015.997868 · 0.58 Impact Factor
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    ABSTRACT: Pharmaceutical compounds have been detected frequently in surface and ground water. Advanced Oxidation Processes (AOPs) were reported as very efficient for removal of various organic compounds. Nevertheless, due to incomplete degradation, toxic intermediates can induce more severe effects than the parent compound. Therefore, toxicity studies are necessary for the evaluation of possible uses of AOPs. In this study the effectiveness and capacity for environmental application of three different AOPs were estimated. They were applied and evaluated for removal of ibuprofen from water solutions. Therefore, two treatments were performed in a non-thermal plasma reactor with dielectric barrier discharge with and without a homogenous catalyst (Fe(2+)). The third treatment was the Fenton reaction. The degradation rate of ibuprofen was measured by HPLC-DAD and the main degradation products were identified using LC-MS TOF. Twelve degradation products were identified, and there were differences according to the various treatments applied. Toxicity effects were determined with two bioassays: Vibrio fischeri and Artemia salina. The efficiency of AOPs was demonstrated for all treatments, where after 15min degradation percentage was over 80% accompanied by opening of the aromatic ring. In the treatment with homogenous catalyst degradation reached 99%. V. fischeri toxicity test has shown greater sensitivity to ibuprofen solution after the Fenton treatment in comparison to A. salina. Copyright © 2014 Elsevier B.V. All rights reserved.
    Science of The Total Environment 11/2014; 505. DOI:10.1016/j.scitotenv.2014.11.017 · 3.16 Impact Factor
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    ABSTRACT: Bentonite clay rich in smectite clay mineral from seldom investigated locality Mečji Do (MD) in Serbia was modified. The organomodification was performed with different loadings of benzyltrimethylammonium (BTMA) cation. The characterization of clay-based samples was performed, including XRD, FTIR and chemical and textural analysis. Electrochemical investigation was performed on a glassy carbon electrode (GCE) support with thin film of homogenously deposited either Na-enriched or one of organomodified clays forming composite electrodes. The behavior of the composite electrodes in the electrooxidation of p-nitrophenol (p-NP) in acidic media was analyzed using multisweep cyclic voltammetry. Oxidation of p-NP occurred at 1.2 V vs. Ag/AgCl for all investigated electrodes. The results indicate that the incorporation of BTMA cations into smectite enhanced the electrode stability toward the electrooxidation of p-NP in comparison to bare GCE and composite electrode based on Na-enriched clay. The current density for the p-NP oxidation wave slightly decreased with the increase of BTMA loading. On the other hand the electrode stability was significantly improved with the increase of BTMA loading.
    Applied Surface Science 09/2014; 313:440-448. DOI:10.1016/j.apsusc.2014.05.228 · 2.54 Impact Factor
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    ABSTRACT: Elevated concentrations of arsenic in groundwater, which is used as a source for drinking water, is a worldwide problem. Use of TiO2 and iron doped TiO2 synthesized by a microwave-assisted hydrothermal method for As(III) and As(V) removal were examined. Synthesized sorbents were characterized with XRD and nitrogen physisorption. Synthesized sorbents have predominantly anatase structure, and no peaks for iron could be observed. Doping of iron increases the surface area of synthesized sorbents. Sorption experiments show that increase of iron in sorbents increases the sorption capacity for As(III) and As(V). Increase of pH from 3 to 11 has no influence on As(III) sorption but decreases the sorption of As(V). Batch isotherm studies were performed to determine the binding capacities of As(III) and As(V). As(III) followed the Freundlich isotherm model, while for As(V) a better fit was with the Langmuir isotherm. The results of competition of SO42– and PO43– anions on adsorption of As(III) indicated that both anions reduced substantially the efficiency of adsorption on both adsorbents while for As(V) only the presence of PO43– anion interfered with adsorption. Testing 10Fe/TiO2 sorbent with arsenic contaminated natural water showed that this material could be used for removal of arsenic to the level recommended by WHO without pretreatment.
    Industrial & Engineering Chemistry Research 07/2014; 53(27):10841–10848. DOI:10.1021/ie500849r · 2.24 Impact Factor
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    ABSTRACT: This paper describes the preparation of a new sensor based on Zn-ferrite modified glassy carbon paste electrode and its electrochemical application for the determination of trace Cd(II) ions in waste waters using differential pulse anodic stripping voltammetry (DPASV). Different Zn/Ni ferrite nanoparticles were synthesized and characterized using scanning electron microscopy (SEM) and X-ray powder diffraction (XRPD). The prepared ferrite nanoparticles were used for the preparation of Zn-ferrite-modified glassy carbon paste electrode (ZnMGCPE) for determination of Cd(II) at nanomolar levels in waste water at pH 5. The different parameters such as conditions of preparation, Zn2+/Ni2+/Fe2+ ratio and electrochemical parameters, percentage of modifier, accumulation time, pH and accumulation potential were investigated. Besides, interference measurements were also evaluated under optimized parameters. The best voltammetric response was observed for ZnFe2O4 modifier, when the percentage of modifier was 3 %, accumulation time 9 min, pH of supporting electrolyte 5 and accumulation potential −1.05 V. Thus prepared electrode displays excellent response to Cd(II) with a detection limit of 0.38 ppb, and selective detection toward Cd(II) was achieved.
    Electroanalysis 07/2014; 26(7). DOI:10.1002/elan.201400095 · 2.50 Impact Factor
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    ABSTRACT: Microwave-hydrothermal method was used for synthesis of TiO2 and TiO2 doped with zirconium. Method was fast and simple and adsorbents were used for removal of As(III) and As(V) from aqueous solutions. The adsorbents were characterized by BET surface area measurements and powder XRD. Experiments showed that TiO2 doped with 10% of Zr using microwave-hydrothermal method have greater specific surface area and total pore volume in comparison with TiO2 synthetized with the same method. Better removal with doped adsorbent was obtained for both, As(III) and As(V). Further experiments were carried out with Zr doped TiO2 sorbent in order to examine kinetic of adsorption, influence of pH and effect of common anions present in natural waters.
    Journal of Saudi Chemical Society 06/2014; DOI:10.1016/j.jscs.2014.05.009 · 1.29 Impact Factor
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    ABSTRACT: An electrochemiluminescent (ECL) swimmer driven by bipolar electrochemistry is reported for enzymatic glucose sensing. The chemo-mechanical motion is induced by localized hydrogen bubble generation. The concomitant oxidation of the luminophore and of the enzymatically-produced NADH leads to ECL emission with a direct glucose-dependent light intensity. We demonstrate herein the local sensing and reporting of glucose in a concentration gradient explored by the ECL swimmer. Such a dynamic sensing approach combines in a synergetic way the wireless propulsion with the enzymatic selectivity using ECL as a readout method at the level of moving objects.
    Chemical Communications 06/2014; 50(71). DOI:10.1039/C4CC04105D · 6.72 Impact Factor
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    ABSTRACT: The remarkable characteristics of electrogenerated chemiluminescence (ECL) as a readout method are successfully exploited in numerous microbead-based immunoassays. However there is still a lack of understanding of the extremely high sensitivity of such ECL bioassays. Here the mechanisms of the reaction of the Ru(bpy)(3)(2+) luminophore with two efficient co-reactants (TPrA or DBAE) were investigated by mapping the ECL reactivity at the level of single Ru(bpy)(3)(2+)-functionalized beads. Micrometric non-conductive beads were decorated with the ruthenium label via a sandwich immunoassay or via a peptide bond. Mapping the ECL reactivity on one bead demonstrates the generation of the excited state at a micrometric distance from the electrode by reaction of surface-confined Ru(bpy)(3)(2+) with diffusing TPrA radicals. The signature of the TPAc center dot+ lifetime is obtained from the ECL profile. Unlike the reaction with Ru(bpy)(3)(2+) in solution, DBAE generates very low ECL intensity in the bead-based format suggesting more unstable radical intermediates. The 3D imaging approach provides insights into the ECL mechanistic route operating in bioassays and on the optical effects that focus the ECL emission.
    Chemical Science 03/2014; 5(6). DOI:10.1039/C4SC00312H · 8.60 Impact Factor
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    ABSTRACT: The aim of this study is to investigate the significance of breast milk and infant formula in the prevention of oxidative stress, by electrochemical determination of the total antioxidant potential, demonstrating the relationship between the antioxidant capacity of milk and postnatal age. Human breast milk, commercial UHT milk, and infant formulas supplemented with prebiotics were used. Samples were diluted in phosphate buffer solution, and the total antioxidant activity was potentiometrically measured by the iodine/iodide redox couple with the Pt Fisher electrode as a working electrode and saturated calomel as a reference electrode. Cyclic voltammograms and differential pulse voltammograms were recorded with the glassy carbon electrode as the working electrode, an accessory platinum electrode, and an Ag/AgCl reference electrode. The potentiometric measurement indicates that human breast milk has the highest redox potential, while the commercial UHT milk has very low potential. Infant formulas also have high potential. The main advantage of electrochemical methods used to assess the total antioxidant activity of milk was that they directly monitored the electron-donating ability of the compounds and could be used for the quantitative analysis of the total antioxidants of different types of milk.
    Food Analytical Methods 02/2014; 7(2). DOI:10.1007/s12161-013-9631-7 · 1.80 Impact Factor
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    01/2014; 1(1). DOI:10.1002/celc.201300042
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    Polish Journal of Environmental Studies 01/2014; 23:2103-2109. DOI:10.15244/pjoes/28298 · 0.60 Impact Factor
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    ABSTRACT: In power plant, coal ash obtained by combustion is mixed with river water and transported to the dump. Sequential extraction was used in order to assess pollution caused by leaching of elements during ash transport through the pipeline and in the storage (cassettes). A total of 80 samples of filter ash as well as the ash from active (currently filled) and passive (previously filled) cassettes were studied. Samples were extracted with distilled water, ammonium acetate, ammonium oxalate/oxalic acid, acidic solution of hydrogen-peroxide, and a hydrochloric acid. Concentrations of the several elements (Al, As, Cd, Co, Cu, Cr, Fe, Ba, Ca, Mg, Ni, Pb, and Zn) in all extracts were determined by inductively coupled plasma atomic emission spectrometry. Pattern recognition method was carried out in order to provide better understanding of the nature of distribution of elements according to their origins. Results indicate possible leaching of As, Ca, Cd, Cu, Zn, and Pb. Among these elements As, Cd, and Pb are toxicologically the most important but they were not present in the first two phases with the exception of As. The leaching could be destructive and cause negative effects on plants, water pollution, and damage to some life forms.
    01/2014; 2014:212506. DOI:10.1155/2014/212506
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    ABSTRACT: Fe-doped TiO 2 photocatalysts with different contents of Fe (0.5, 1.6, 3.4 and 6.4 %) were synthesized by a microwave-hydrothermal method and characterized by X-ray diffraction analysis, N 2 physisorption at 77 K and UV– –Vis spectrometry. The characterization showed that the Fe ions were highly dispersed in the TiO 2 lattice. It was found that all the synthesized catalysts had a mesoporous structure and that Fe-doping increased the BET surface area. The UV–Vis study showed that the absorption spectra were shifted to longer wave-lengths (red shift) with increasing dopant concentration. The photocatalytic activity of the samples was evaluated by the decolorization of the textile dye Reactive Blue 52 (RB) in aqueous solutions under sun-like radiation in the presence of H 2 O 2 (a heterogeneous photo-Fenton process). The photocatalyst with 3.4 % Fe was found to be the most efficient in the presence of H 2 O 2 . The effect of the initial pH of the dye solution was assessed and dissolution of iron ions was studied as a function of pH value. It was concluded that decolori-zation was more favorable in acidic pH, and that at pH values >4, the release of Fe ions into the solution was negligible. Photocatalytic degradation of 4-chlorophenol (4-CP) was investigated under the optimal conditions and it was shown that the catalyst was capable of degrading colorless pollutants.
    Journal of the Serbian Chemical Society 11/2013; 79021(8):977-991. DOI:10.2298/JSC131001143N · 0.89 Impact Factor
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    ABSTRACT: Degradation of two triketone herbicides, mesotrione and sulcotrione, was studied using four different advanced oxidation processes (AOPs): ozonization, dielectric barrier discharge (DBD reactor), photocatalysis and Fenton reagent, in order to find differences in mechanism of degradation. Degradation products were identified by high performance liquid chromatography (HPLC-DAD) and UHPLC-Orbitrap-MS analyses. A simple mechanism of degradation for different AOP was proposed. Thirteen products were identified during all degradations for both pesticides. It was assumed that the oxidation mechanisms in the all four technologies were not based only on the production and use of the hydroxyl radical, but they also included other kinds of oxidation mechanisms specific for each technology. Similarity was observed between degradation mechanism of ozonation and DBD. The greatest difference in the products was found in Fenton degradation which included the opening of benzene ring. When degraded with same AOP pesticides gave at the end of treatment the same products. Global toxicity and COD value of samples was determined after all degradations. Real water sample was used to study influence of organic matter on pesticide degradation. These results could lead to accurate estimates of the overall effects of triketone herbicides on environmental ecosystems and also contributed to the development of improved removal processes.
    Journal of hazardous materials 07/2013; 260C:1092-1099. DOI:10.1016/j.jhazmat.2013.06.073 · 4.33 Impact Factor
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    Dataset: ECLfinal7
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    ABSTRACT: The concentrations of iron, lead, cadmium, copper, manganese, mercury and arsenic were measured in water, sediment, five macrophytes (Typha angustifolia, Iris pseudacorus, Polygonum amphybium, Myriophyllum spicatum and Lemna gibba) and five fish species (Sander lucioperca, Abramis brama, Carassius gibelio, Silurus glanis and Arystichtys nobilis) in the Gruža Reservoir, used for water supply and recreational fishing. The concentrations of all examined elements were higher in sediment than in water. The values of the ratio between element concentrations in the sediment and those in the water were the highest for Fe and As. Among the five plant species, the highest concentrations of Pb and Mn were observed in T. angustifolia, while the highest concentrations of Fe, Cu and Hg were in L. gibba. I. pseudacorus and P. amphybium had the highest concentrations of Cd and As, respectively. Among the fish species, C. gibelio showed the highest tendency of element accumulation (Fe, Cd, Cu), followed by S. lucioperca (Pb, Hg), A. brama (Mn) and A. nobilis (As). The average concentrations of elements in fish muscle, except for As in A. nobilis (2.635 ± 0.241 mg kg(-1) ww), were below the limits that are considered safe for human consumption in accordance with the European Commission Regulation and Official Gazette of Serbia.
    Bulletin of Environmental Contamination and Toxicology 02/2013; DOI:10.1007/s00128-013-0969-8 · 1.22 Impact Factor
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    ABSTRACT: Ellagic acid is a biologically active polyphenol found in numerous fruits and vegetables. However, not many papers dealing with the electrochemical properties and protolytic equilibria of ellagic acid have been published so far. The electro-oxidation mechanism of ellagic acid was studied in methanol aqueous media (1:1, v/v) within the pH range of 1.5–9.0, t = 25 ± 1 °C, using cyclic voltammetry on a glassy carbon electrode, and by semiempirical calculations. Results show that oxidation of ellagic acid is a pH-dependent, two-step quasireversible process. The slope of peak 1 indicates the exchange of the same number of electrons and protons within the whole studied pH range; the slope of peak 2 changes with the increase of pH, and three different regions are visible. As protolytic equilibria studies revealed that ellagic acid acts as a diprotic acid in the studied conditions (acidity constants were potentiometrically determined as pK a1 = 5.42 ± 0.01 and pK a2 = 6.76 ± 0.01), it is obvious that the electro-oxidation occurs at the hydroxyl group subjected to dissociation. The three different regions are therefore recognized as regions with different dominating species: unionized molecule (H4A), monoanion (H3A−), and dianion (H2A2−). UV/Vis spectral changes confirmed the proposed equilibria. Heat of formation and electron densities calculated at semiempirical level were used to propose the hydrogen and electron abstraction sites. According to the obtained results, a new mechanism of ellagic acid electro-oxidation is proposed.